AB30554

Anti-14-3-3 (phospho S58) antibody

Name: Anti-14-3-3 (phospho S58) antibody (ab30554) | Abcam
Brand: Abcam Limited
SKU: AB30554
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal 1433B phospho S58 antibody. Suitable for WB, IHC-Fr and reacts with Mouse, Rat, Zebrafish samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human YWHAB phospho S58.

View Alternative Names

14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB

3 Images

Immunohistochemistry (Frozen sections) - Anti-14-3-3 (phospho S58) antibody (AB30554)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-14-3-3 (phospho S58) antibody (AB30554)

ab30554 staining 14-3-3 (phospho S58) in Mouse hippocampal tissue by Immunohistochemistry (Frozen sections). ab30554 is green, red staining is GFAP and nuclei are stained blue with DAPI.

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Western blot - Anti-14-3-3 (phospho S58) antibody (AB30554)

All lanes:

Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554) at 1/1000 dilution

Lane 1:

Rat brain stem lysate

Lane 2:

Rat brain stem lysate with the phosphopeptide used as the antigen

Predicted band size: 28 kDa

Observed band size: 29 kDa

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Western blot - Anti-14-3-3 (phospho S58) antibody (AB30554)

All lanes:

Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554) at 1 µg/mL

Lane 2:

Zebrafish brain homogenate (20ug)

Lane 3:

Mouse brain homogenate (20ug)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Zebrafish

Applications

WB, IHC-Fr

applications

Immunogen

Synthetic Peptide within Human YWHAB phospho S58. The exact immunogen used to generate this antibody is proprietary information.

P31946

Specificity

Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

The N-terminal sequence used as antigen has high homology across a wide variety of species for all of the 14-3-3 proteins. We have not specifically tested it for reactivity against each protein in any given species, we do expect it to recognize most all of them.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Prepared from rabbit serum by affinity purification via sequentialchromatography on phospho- and dephosphopeptide affinity columns.

Storage buffer

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 proteins are a family of regulatory molecules with a molecular mass around 28 to 32 kDa. These proteins exist in seven isoforms in humans termed beta gamma epsilon zeta eta theta and sigma. 14-3-3 proteins are ubiquitously expressed in eukaryotic cells including neurons liver cells and immune cells. These proteins participate in modulation of many cellular processes by binding to phosphorylated serine and threonine residues on various target proteins. This binding alters the conformation of target proteins influencing their activity subcellular localization or stability.

Biological function summary

14-3-3 proteins are involved in signal transduction cell cycle control and apoptosis regulation. They participate in protein interactions and form dimers that serve as scaffolds assembling complexes and linking signaling pathways. The proteins associate with kinases such as Raf-1 thereby contributing to signaling pathways that include MAPK/ERK. At the cellular level 14-3-3 proteins govern key mechanisms like the G1/S phase transition and stress response which are critical for maintaining cellular homeostasis.

Pathways

14-3-3 proteins play critical roles in the PI3K/AKT and MAPK/ERK pathways. They interact with several key proteins within these pathways including AKT and Raf-1 mediating cellular responses to growth factors and stress. In the PI3K/AKT pathway 14-3-3 proteins help regulate the progression of the cell cycle and promote cell survival linking extracellular signals to the machinery that governs cell fate. These interactions underline the role of 14-3-3 proteins in integrating and regulating complex cell signaling networks.

14-3-3 proteins are associated with neurodegenerative diseases like Alzheimer's and certain types of cancer. In Alzheimer's disease 14-3-3 proteins interact with tau protein contributing to the pathological aggregation of tau associated with neuronal damage. Similarly in various cancers their interaction with cell cycle regulators like p53 can influence tumor progression by affecting cell division and apoptosis. These connections highlight the involvement of 14-3-3 proteins in disease mechanisms making them potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.

See full target information YWHAB phospho S58

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Brain research 1778:147781 PubMed35016853

2022

The Roc domain of LRRK2 as a hub for protein-protein interactions: a focus on PAK6 and its impact on RAB phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Susanna Cogo,Franz Y Ho,Elena Tosoni,James E Tomkins,Isabella Tessari,Lucia Iannotta,Thomas J Montine,Claudia Manzoni,Patrick A Lewis,Luigi Bubacco,Marie-Christine Chartier Harlin,Jean-Marc Taymans,Arjan Kortholt,Jeremy Nichols,Laura Cendron,Laura Civiero,Elisa Greggio

Frontiers in molecular neuroscience 10:417 PubMed29311810

2017

PAK6 Phosphorylates 14-3-3γ to Regulate Steady State Phosphorylation of LRRK2.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Civiero,Susanna Cogo,Anneleen Kiekens,Claudia Morganti,Isabella Tessari,Evy Lobbestael,Veerle Baekelandt,Jean-Marc Taymans,Marie-Christine Chartier-Harlin,Cinzia Franchin,Giorgio Arrigoni,Patrick A Lewis,Giovanni Piccoli,Luigi Bubacco,Mark R Cookson,Paolo Pinton,Elisa Greggio

Journal of cell science 124:57-67 PubMed21118956

2010

A novel Chk1/2-Lats2-14-3-3 signaling pathway regulates P-body formation in response to UV damage.

Applications

ICC/IF, WB

Species

Human, Human

Nobuhiro Okada,Norikazu Yabuta,Hirokazu Suzuki,Yael Aylon,Moshe Oren,Hiroshi Nojima

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 (phospho S58) antibody

Immunohistochemistry (Frozen sections) - Anti-14-3-3 (phospho S58) antibody (AB30554)

Western blot - Anti-14-3-3 (phospho S58) antibody (AB30554)

Western blot - Anti-14-3-3 (phospho S58) antibody (AB30554)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise