JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB151504

Anti-14-3-3 sigma/SFN antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal 1433S antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SFN aa 150 to C-terminus.

View Alternative Names

HME1, SFN, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB151504)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB151504)

Immunohistochemical analysis of paraffin-embedded Human 59T xenograft tissue, labeling 14-3-3 sigma/SFN with ab151504 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 sigma/SFN antibody (AB151504)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 sigma/SFN antibody (AB151504)

Immunofluorescent analysis of A431 cells (fixed in ice-cold Methanol for 5 min), labeling 14-3-3 sigma/SFN with ab151504 at 1/500 dilution. The right image shows cells co-stained with Hoechst 33343.

Western blot - Anti-14-3-3 sigma/SFN antibody (AB151504)
  • WB

Supplier Data

Western blot - Anti-14-3-3 sigma/SFN antibody (AB151504)

12% SDS PAGE

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Predicted band size: 27 kDa

false

Western blot - Anti-14-3-3 sigma/SFN antibody (AB151504)
  • WB

Unknown

Western blot - Anti-14-3-3 sigma/SFN antibody (AB151504)

12% SDS PAGE

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution

All lanes:

PC12 whole cell lysate at 30 µg

Predicted band size: 27 kDa

false

Western blot - Anti-14-3-3 sigma/SFN antibody (AB151504)
  • WB

Unknown

Western blot - Anti-14-3-3 sigma/SFN antibody (AB151504)

12% SDS PAGE

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human SFN aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P31947

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Or perform heat mediated retrieval with Tris-EDTA buffer pH 8</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.
Biological function summary

14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.

Pathways

14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.

Disruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed : 37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed : 9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed : 24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed : 29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed : 18382127).
See full target information SFN
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Chemical Society 146:24788-24799 PubMed39196545

2024

Targeted Protein Localization by Covalent 14-3-3 Recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Shao,Tuong Nghi Duong,Inji Park,Lauren M Orr,Daniel K Nomura

Medical science monitor : international medical jo 23:3861-3868 PubMed28794404

2017

Role of Epithelial Cell Transforming Sequence 2 (ECT2) in Predicting Prognosis of Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiguo Chen,Jiatian Liu,Yihang Zhang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB268067

Anti-14-3-3 sigma/SFN antibody [CPTC-SFN-2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [CPTC-SFN-2] (AB268067)

  • 0
  • 0
Primary Antibodies

AB86380

Anti-14-3-3 sigma/SFN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB86380)

  • 0
  • 0
Primary Antibodies

AB77187

Anti-14-3-3 sigma/SFN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB77187)

  • 0
  • 0
Primary Antibodies

AB193667

Anti-14-3-3 sigma/SFN antibody [EPR5873(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

  • 0
  • 0

Complementary Products

Assay Kits

AB245711

Human SFN ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SFN ELISA Kit (AB245711)

  • 0
  • 0
Primary Antibodies

AB108349

Anti-CDKN2A/p16INK4a antibody [EPR1473] - C-terminal

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [EPR1473] - C-terminal (AB108349)

  • 4
  • 7
Primary Antibodies

AB124762

Anti-p63 antibody [EPR5701]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] (AB124762)

  • 4
  • 12
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB188224

Anti-p21 antibody [EPR18021]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p21 antibody [EPR18021] (AB188224)

  • 5
  • 11

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com