Rabbit Polyclonal 1433S antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human 14-3-3 protein sigma aa 150 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Or perform heat mediated retrieval with Tris-EDTA buffer pH 8 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed:37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed:9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed:24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed:29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed:18382127).
HME1, HME1, SFN, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin
Rabbit Polyclonal 1433S antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human 14-3-3 protein sigma aa 150 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.
14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.
14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.
Disruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.
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12% SDS PAGE
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution
Lane 1: Neuro2A whole cell lysate at 30 µg
Lane 2: GL261 whole cell lysate at 30 µg
Predicted band size: 27 kDa
12% SDS PAGE
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution
All lanes: PC12 whole cell lysate at 30 µg
Predicted band size: 27 kDa
12% SDS PAGE
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody (ab151504) at 1/3000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Lane 4: HepG2 whole cell lysate at 30 µg
Predicted band size: 27 kDa
Immunofluorescent analysis of A431 cells (fixed in ice-cold Methanol for 5 min), labeling 14-3-3 sigma/SFN with ab151504 at 1/500 dilution. The right image shows cells co-stained with Hoechst 33343.
Immunohistochemical analysis of paraffin-embedded Human 59T xenograft tissue, labeling 14-3-3 sigma/SFN with ab151504 at 1/100 dilution.
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