Goat Polyclonal 1433S antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SFN.
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- EMBO molecular medicine 13:e128712021Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristoph H Mayr,Lukas M Simon,Gabriela Leuschner,Meshal Ansari,Janine Schniering,Philipp E Geyer,Ilias Angelidis,Maximilian Strunz,Pawandeep Singh,Nikolaus Kneidinger,Frank Reichenberger,Edith Silbernagel,Stephan Böhm,Heiko Adler,Michael Lindner,Britta Maurer,Anne Hilgendorff,Antje Prasse,Jürgen Behr,Matthias Mann,Oliver Eickelberg,Fabian J Theis,Herbert B SchillerPubMed 33650774
- Nature cell biology 22:934-9462020Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshihiko Kobayashi et. al.PubMed 32661339
- Molecular biology of the cell 25:4195-2042014Rap1-dependent pathways coordinate cytokinesis in Dictyostelium.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SFN. The exact immunogen used to generate this antibody is proprietary information. Database link P31947
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Sheep | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.03000-0.10000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Rat | Dilution info 0.03000-0.10000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Human | Dilution info 0.03000-0.10000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Dog, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed:37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed:9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed:24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed:29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed:18382127).
Alternative names
HME1, SFN, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin
Recommended products
Goat Polyclonal 1433S antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SFN.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SFN. The exact immunogen used to generate this antibody is proprietary information. Database link P31947
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.
- Biological function summary
14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.
- Pathways
14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.
- Associated diseases and disorders
Disruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)
Primary incubation was 1 hour. Detected using chemiluminescence.
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/mL
All lanes: Human skin tissue lysate in RIPA buffer at 35 µg
Predicted band size: 27 kDa
Observed band size: 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (ab77187)
Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma/SFN with ab77187 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)
Primary incubation was 1 hour. Detected using chemiluminescence.
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/mL
Lane 1: A431 cell lysate in RIPA buffer at 35 µg
Lane 2: U2OS cell lysate in RIPA buffer at 35 µg
Predicted band size: 27 kDa
Observed band size: 27 kDa
Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)
Primary incubation was 1 hour. Detected using chemiluminescence.
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/mL
Lane 1: Mouse skin tissue lysate in RIPA buffer at 35 µg
Lane 2: Rat skin tissue lysate in RIPA buffer at 35 µg
Predicted band size: 27 kDa
Observed band size: 27 kDa
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