Rabbit Recombinant Monoclonal 1433S antibody. Suitable for WB, IHC-P and reacts with Synthetic peptide - Human, Human, Synthetic peptide samples. Cited in 2 publications.
Images
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-ab193667--western-blot-img66132.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-ab193667--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38861.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)")
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-ab193667--western-blot-img14069.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)")
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-ab193667--western-blot-img415964.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)")
Publications
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- International journal of genomics 2023:97316752023Stratifin Promotes Hepatocellular Carcinoma Progression by Modulating the Wnt/-Catenin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan-Ping Ye,Hong-Xin Yu,Wei-Jie Lu,Jun-Fu Wang,Tai-Yuan Li,Jun Shi,Xiao-Ye ChengPubMed 37587914
- Journal of cellular and molecular medicine 25:3524-35362021ARID1B/SUB1-activated lncRNA HOXA-AS2 drives the malignant behaviour of hepatoblastoma through regulation of HOXA3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGongbao Liu,Baihui Liu,Xiangqi Liu,Lulu Xie,Jiajun He,Jingjing Zhang,Rui Dong,Duan Ma,Kuiran Dong,Mujie YePubMed 33683826
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Synthetic peptide | Not recommended | Tested |
| Synthetic peptide - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/1000 - 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/600 | Notes - |
Species Human | Dilution info 1/600 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed:37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed:9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed:24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed:29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed:18382127).
Alternative names
HME1, SFN, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin
Recommended products
Rabbit Recombinant Monoclonal 1433S antibody. Suitable for WB, IHC-P and reacts with Synthetic peptide - Human, Human, Synthetic peptide samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5873(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.
- Biological function summary
14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.
- Pathways
14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.
- Associated diseases and disorders
Disruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-ab193667--western-blot-img66132.jpg "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)")
Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution
Lane 1: Human skin lysate at 20 µg
Lane 2: A431 cell lysate at 20 µg
Lane 3: HACAT cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-ab193667--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38861.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 14-3-3 sigma/SFN with ab193667 at 1/600 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-ab193667--western-blot-img14069.jpg "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)")
Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
Human full length 14-3-3 sigma/SFN recombinant protein is a house-made one with His-Tag.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution
All lanes: Human 14-3-3 sigma/SFN recombinant protein with His tag at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-ab193667--western-blot-img415964.png "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)")
Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667)
Blocking and dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution
Lane 1: 14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged. at 10 ng
Lane 2: 14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 3: 14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 4: 14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 5: 14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 6: 14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 7: 14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa
Exposure time: 60s
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