AB193667

Anti-14-3-3 sigma/SFN antibody [EPR5873(2)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Recombinant Monoclonal 1433S antibody. Suitable for WB, IHC-P and reacts with Synthetic peptide - Human, Human, Synthetic peptide samples. Cited in 5 publications.

View Alternative Names

HME1, SFN, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 14-3-3 sigma/SFN with ab193667 at 1/600 dilution (2μg/ml) followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Human full length 14-3-3 sigma/SFN recombinant protein is a house-made one with His-Tag.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution

All lanes:

Human 14-3-3 sigma/SFN recombinant protein with His tag at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution

Lane 1:

Human skin lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

HACAT cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution

Lane 1:

14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 2:

14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 3:

14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 4:

14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 5:

14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 6:

14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 7:

14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 27 kDa

Observed band size: 28 kDa

false

Exposure time: 60s

Carrier free

Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5873(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.

Biological function summary

14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.

Pathways

14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.

Disruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed : 37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed : 9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed : 24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed : 29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed : 18382127).

See full target information SFN

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 40:140-151 PubMed39415627

2024

H3K27 Acetylation-Activated GLDC Accelerated the Advancement of Oral Squamous Cell Carcinoma by Suppressing the p53 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Xu,Qingfeng Xu,Haibing Yang

The Journal of cell biology 223: PubMed38625077

2024

Determinants of minor satellite RNA function in chromosome segregation in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Li Chen,Alisha N Jones,Amy Crawford,Michael Sattler,Andreas Ettinger,Maria-Elena Torres-Padilla

International journal of genomics 2023:9731675 PubMed37587914

2023

Stratifin Promotes Hepatocellular Carcinoma Progression by Modulating the Wnt/-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shan-Ping Ye,Hong-Xin Yu,Wei-Jie Lu,Jun-Fu Wang,Tai-Yuan Li,Jun Shi,Xiao-Ye Cheng

Pharmaceuticals (Basel, Switzerland) 15: PubMed35890172

2022

ZLM-7 Blocks Breast Cancer Progression by Inhibiting MDM2 via Upregulation of 14-3-3 Sigma.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wen,Zi-Zheng Zou,Tiao Luo,Xuan Li,Su-You Liu,Ji-Jia Li,Zhi-Yong Luo

Journal of cellular and molecular medicine 25:3524-3536 PubMed33683826

2021

ARID1B/SUB1-activated lncRNA HOXA-AS2 drives the malignant behaviour of hepatoblastoma through regulation of HOXA3.

Applications

Unspecified application

Species

Unspecified reactive species

Gongbao Liu,Baihui Liu,Xiangqi Liu,Lulu Xie,Jiajun He,Jingjing Zhang,Rui Dong,Duan Ma,Kuiran Dong,Mujie Ye

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 sigma/SFN antibody [EPR5873(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise