Rabbit Recombinant Monoclonal 1433S antibody. Carrier free. Suitable for WB, IHC-P and reacts with Synthetic peptide - Human, Human, Synthetic peptide samples.
Images
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (AB251175), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-bsa-and-azide-free-ab251175--western-blot-img66132.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (AB251175)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (AB251175), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-bsa-and-azide-free-ab251175--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38861.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (AB251175)")
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (AB251175), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-bsa-and-azide-free-ab251175--western-blot-img14069.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (AB251175)")
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (AB251175), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-bsa-and-azide-free-ab251175--western-blot-img415964.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (AB251175)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Synthetic peptide | Not recommended | Tested |
| Synthetic peptide - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Synthetic peptide | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed:37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed:9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed:24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed:29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed:18382127).
Alternative names
HME1, SFN, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin
Recommended products
Rabbit Recombinant Monoclonal 1433S antibody. Carrier free. Suitable for WB, IHC-P and reacts with Synthetic peptide - Human, Human, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5873(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251175 is the carrier-free version of Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] ab193667.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.
- Biological function summary
14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.
- Pathways
14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.
- Associated diseases and disorders
Disruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-bsa-and-azide-free-ab251175--western-blot-img66132.jpg "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)")
Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
This data was developed using Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] ab193667, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] ab193667) at 1/1000 dilution
Lane 1: Human skin lysate at 20 µg
Lane 2: A431 cell lysate at 20 µg
Lane 3: HACAT cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-bsa-and-azide-free-ab251175--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38861.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
This data was developed using Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] ab193667, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 14-3-3 sigma/SFN with Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] ab193667 at 1/600 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-bsa-and-azide-free-ab251175--western-blot-img14069.jpg "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)")
Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
This data was developed using Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] ab193667, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human full length 14-3-3 sigma/SFN recombinant protein is a house-made one with His-Tag.
All lanes: Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] ab193667) at 1/1000 dilution
All lanes: Human 14-3-3 sigma/SFN recombinant protein with His tag at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa
![Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-sigma-sfn-antibody-epr58732-bsa-and-azide-free-ab251175--western-blot-img415964.png "Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)")
Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free (ab251175)
This data was developed using Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] ab193667, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] ab193667) at 1/1000 dilution
Lane 1: 14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 2: 14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 3: 14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 4: 14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 5: 14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 6: 14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 7: 14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa
Exposure time: 60s
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