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AB183075

Anti-14-3-3 theta antibody

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(2 Publications)

Rabbit Polyclonal 14-3-3 Theta + Tau antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human 14-3-3 protein theta aa 100-200.

View Alternative Names

14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 theta antibody (AB183075)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 theta antibody (AB183075)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 14-3-3 theta with ab183075 at a 1/20 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification : ×200)

Western blot - Anti-14-3-3 theta antibody (AB183075)
  • WB

Supplier Data

Western blot - Anti-14-3-3 theta antibody (AB183075)

All lanes:

Western blot - Anti-14-3-3 theta antibody (ab183075) at 1/300 dilution

Lane 1:

mouse brain tissue lysate at 40 µg

Lane 2:

Human A549 cell lysate at 40 µg

Lane 3:

Human lymphoma tissue lysate at 40 µg

Lane 4:

mouse NIH 3T3 cell lysate at 40 µg

Lane 5:

Human colon cancer tissue lysate at 40 µg

Secondary

All lanes:

Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

Predicted band size: 28 kDa

true

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human 14-3-3 protein theta aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

P27348

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Complementary Products

Assay Kits

AB314823

Human 14-3-3 theta ELISA Kit

Sandwich ELISA - Human 14-3-3 theta ELISA Kit (AB314823)

  • 0
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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Reagents, Controls & Accessories

AB214286

Protein A Magnetic Beads

  • 0
  • 0
Primary Antibodies

AB211033

Anti-SIRT2 antibody [EPR20411-105]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody [EPR20411-105] (AB211033)

  • 4
  • 8
Primary Antibodies

AB178677

Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 theta also known as YWHAQ is a member of the 14-3-3 protein family with a mass of approximately 28 kDa. These proteins are known for binding phosphoserine-containing proteins. In the case of 14-3-3 theta it is involved in the regulation of a broad range of proteins by altering activity cellular localization and stability. This protein is widely expressed in many tissues but has high expression levels in the brain indicating its involvement in neural functions.
Biological function summary

14-3-3 theta plays key roles in various signaling pathways and cellular processes. It participates as a scaffold or adapter molecule in the formation of protein complexes aiding in signal transduction. Its ability to bind to numerous client proteins allows it to regulate biological processes such as apoptosis cell cycle progression and cellular stress responses. By facilitating these processes 14-3-3 theta maintains cellular homeostasis and proper cellular responses to environmental cues.

Pathways

14-3-3 theta integrally functions within the MAPK and PI3K/Akt signaling pathways. These pathways involve the regulation of cell growth survival and metabolism. In the MAPK pathway 14-3-3 theta collaborates with proteins like Raf and MEK assisting in the transduction of growth signals. In the PI3K/Akt pathway it interacts with Akt influencing cell survival and proliferation by modulating downstream targets.

Dysregulation of 14-3-3 theta has associations with neurodegenerative disorders like Alzheimer's disease and psychiatric disorders such as schizophrenia. In Alzheimer's disease interactions with tau protein are noteworthy which may affect tau phosphorylation and subsequent aggregation. In schizophrenia alterations in signaling pathways where 14-3-3 theta is an active participant might cause disruptions in neural signaling contributing to the disorder's pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
See full target information 14-3-3 protein theta
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed38399439

2024

Arnicolide C Suppresses Tumor Progression by Targeting 14-3-3θ in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengrui Liu,Xiaodan Lyu,Jiaxu Chen,Benteng Zhang,Siman Xie,Yan Yuan,Li Sun,Shengtao Yuan,Hong Yu,Jian Ding,Mei Yang

Animals : an open access journal from MDPI 11: PubMed34573602

2021

Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ilona Kowalczyk-Zieba,Joanna Staszkiewicz-Chodor,Dorota Boruszewska,Krzysztof Lukaszuk,Joanna Jaworska,Izabela Woclawek-Potocka
View all publications
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Product promise

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