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AB252649

Anti-14-3-3 Theta + Tau antibody [EPR20720-227] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal 14-3-3 Theta + Tau antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ

1 Images
Sandwich ELISA - Anti-14-3-3 Theta + Tau antibody [EPR20720-227] - BSA and Azide free (Detector) (AB252649)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-14-3-3 Theta + Tau antibody [EPR20720-227] - BSA and Azide free (Detector) (AB252649)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab246543).

  • Biotin

    Biotin Anti-14-3-3 Theta + Tau antibody [EPR20720-227] (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20720-227

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab252649 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human 14-3-3 theta + tau. The recommended pair for sandwich ELISA is:
Capture: ab252590, Human 14-3-3 tau Capture Antibody (unconjugated)
Detector: ab252649, Human 14-3-3 tau Detector Antibody (unconjugated)
The reference range value is 23.4 - 1500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 Theta also known as 14-3-3 protein theta is a member of the 14-3-3 protein family which consists of seven distinct isoforms in humans. It acts as an adaptor or scaffold aiding in the regulation of signal transduction pathways by binding to phosphoserine-containing proteins. The protein typically has a molecular mass of approximately 28-32 kDa. It is abundantly expressed in various tissues with a high concentration in the brain where it interacts with a number of neuronal proteins.
Biological function summary

14-3-3 Theta plays a significant role in cell cycle regulation apoptosis and brain development. It often forms part of larger protein complexes acting as a bridge to facilitate interactions between different proteins. The protein aids in maintaining cellular homeostasis and mediates various signal transduction processes by changing the conformation of its binding partners which influences their activity.

Pathways

14-3-3 Theta is actively involved in the MAPK/ERK and PI3K/AKT pathways which are critical for cell proliferation differentiation and survival. Through these pathways the protein interacts with a variety of partners including thr-308-phosphorylated Akt and Raf kinases. 14-3-3 Theta's ability to bind different phosphorylated targets enables it to influence diverse cellular processes within these pathways.

14-3-3 Theta has been linked to neurodegenerative diseases such as Alzheimer's and Parkinson's. Notably its interaction with tau protein plays an important role in Alzheimer's disease as abnormal phosphorylation and aggregation of tau is a characteristic of the disorder. Furthermore disruptions in 14-3-3 Theta function have connections to cancer by interacting with signaling proteins like the tumor suppressor p53 leading to altered cell growth and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
See full target information 14-3-3 protein theta
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com