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AB124909

Anti-14-3-3 Theta + Tau antibody [EPR5123]

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(1 Publication)

Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

4 Images
Flow Cytometry (Intracellular) - Anti-14-3-3 Theta + Tau antibody [EPR5123] (AB124909)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-14-3-3 Theta + Tau antibody [EPR5123] (AB124909)

Intracellular flow cytometric analysis of permeablized A549 cells using ab124909 at 1/500 dilution (red) or a rabbit IgG negative control (green).

Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] (AB124909)
  • WB

Unknown

Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] (AB124909)

All lanes:

Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] (AB124909)
  • WB

Supplier Data

Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] (AB124909)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909) at 1/1000 dilution

Lane 1:

14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 2:

14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 3:

14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged at 10 EU/ng

Lane 4:

14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 5:

14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 6:

14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 7:

14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 3s

OI-RD Scanning - Anti-14-3-3 Theta + Tau antibody [EPR5123] (AB124909)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-14-3-3 Theta + Tau antibody [EPR5123] (AB124909)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5123

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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  • 5
  • 2
Primary Antibodies

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Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (AB108387)

  • 2
  • 2
Primary Antibodies

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Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody [EPR27044-19] - BSA and Azide free (AB317439)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500 - 1/1000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 Theta also known as 14-3-3 protein theta is a member of the 14-3-3 protein family which consists of seven distinct isoforms in humans. It acts as an adaptor or scaffold aiding in the regulation of signal transduction pathways by binding to phosphoserine-containing proteins. The protein typically has a molecular mass of approximately 28-32 kDa. It is abundantly expressed in various tissues with a high concentration in the brain where it interacts with a number of neuronal proteins.
Biological function summary

14-3-3 Theta plays a significant role in cell cycle regulation apoptosis and brain development. It often forms part of larger protein complexes acting as a bridge to facilitate interactions between different proteins. The protein aids in maintaining cellular homeostasis and mediates various signal transduction processes by changing the conformation of its binding partners which influences their activity.

Pathways

14-3-3 Theta is actively involved in the MAPK/ERK and PI3K/AKT pathways which are critical for cell proliferation differentiation and survival. Through these pathways the protein interacts with a variety of partners including thr-308-phosphorylated Akt and Raf kinases. 14-3-3 Theta's ability to bind different phosphorylated targets enables it to influence diverse cellular processes within these pathways.

14-3-3 Theta has been linked to neurodegenerative diseases such as Alzheimer's and Parkinson's. Notably its interaction with tau protein plays an important role in Alzheimer's disease as abnormal phosphorylation and aggregation of tau is a characteristic of the disorder. Furthermore disruptions in 14-3-3 Theta function have connections to cancer by interacting with signaling proteins like the tumor suppressor p53 leading to altered cell growth and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 128:992-1000 PubMed25588844

2015

Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils.

Applications

WB

Species

Human

Kun Gao,Wenwen Tang,Yuan Li,Pingzhao Zhang,Dejie Wang,Long Yu,Chenji Wang,Dianqing Wu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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