Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (AB248055), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-theta-tau-antibody-epr5123-bsa-and-azide-free-ab248055--western-blot-img115487.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (AB248055)")
![Flow Cytometry (Intracellular) - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (AB248055), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-theta-tau-antibody-epr5123-bsa-and-azide-free-ab248055--flow-cytometry-intracellular-img37492.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (AB248055)")
![OI-RD Scanning - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (AB248055), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-theta-tau-antibody-epr5123-bsa-and-azide-free-ab248055--oi-rd-scanning-img84298.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (AB248055)")
![Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (AB248055), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-theta-tau-antibody-epr5123-bsa-and-azide-free-ab248055--western-blot-img415951.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (AB248055)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Predicted | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Additional Targets
MAPT
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5123
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 6.09 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248055 is the carrier-free version of Anti-14-3-3 Theta + Tau antibody [EPR5123] ab124909.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
14-3-3 Theta also known as 14-3-3 protein theta is a member of the 14-3-3 protein family which consists of seven distinct isoforms in humans. It acts as an adaptor or scaffold aiding in the regulation of signal transduction pathways by binding to phosphoserine-containing proteins. The protein typically has a molecular mass of approximately 28-32 kDa. It is abundantly expressed in various tissues with a high concentration in the brain where it interacts with a number of neuronal proteins.
- Biological function summary
14-3-3 Theta plays a significant role in cell cycle regulation apoptosis and brain development. It often forms part of larger protein complexes acting as a bridge to facilitate interactions between different proteins. The protein aids in maintaining cellular homeostasis and mediates various signal transduction processes by changing the conformation of its binding partners which influences their activity.
- Pathways
14-3-3 Theta is actively involved in the MAPK/ERK and PI3K/AKT pathways which are critical for cell proliferation differentiation and survival. Through these pathways the protein interacts with a variety of partners including thr-308-phosphorylated Akt and Raf kinases. 14-3-3 Theta's ability to bind different phosphorylated targets enables it to influence diverse cellular processes within these pathways.
- Associated diseases and disorders
14-3-3 Theta has been linked to neurodegenerative diseases such as Alzheimer's and Parkinson's. Notably its interaction with tau protein plays an important role in Alzheimer's disease as abnormal phosphorylation and aggregation of tau is a characteristic of the disorder. Furthermore disruptions in 14-3-3 Theta function have connections to cancer by interacting with signaling proteins like the tumor suppressor p53 leading to altered cell growth and survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-theta-tau-antibody-epr5123-bsa-and-azide-free-ab248055--western-blot-img115487.jpg "Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055)")
Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055)
This data was developed using Anti-14-3-3 Theta + Tau antibody [EPR5123] ab124909, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] (Anti-14-3-3 Theta + Tau antibody [EPR5123] ab124909) at 1/10000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 3: A549 cell lysate at 10 µg
Lane 4: A431 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
![Flow Cytometry (Intracellular) - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-theta-tau-antibody-epr5123-bsa-and-azide-free-ab248055--flow-cytometry-intracellular-img37492.jpg "Flow Cytometry (Intracellular) - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055)")
Flow Cytometry (Intracellular) - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055)
This data was developed using Anti-14-3-3 Theta + Tau antibody [EPR5123] ab124909, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeablized A549 cells using Anti-14-3-3 Theta + Tau antibody [EPR5123] ab124909 at 1/500 dilution (red) or a rabbit IgG negative control (green).
![OI-RD Scanning - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-theta-tau-antibody-epr5123-bsa-and-azide-free-ab248055--oi-rd-scanning-img84298.jpg "OI-RD Scanning - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055)")
OI-RD Scanning - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055), expandable thumbnail](https://content.abcam.com/products/images/14-3-3-theta-tau-antibody-epr5123-bsa-and-azide-free-ab248055--western-blot-img415951.png "Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055)")
Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] - BSA and Azide free (ab248055)
This data was developed using Anti-14-3-3 Theta + Tau antibody [EPR5123] ab124909, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] (Anti-14-3-3 Theta + Tau antibody [EPR5123] ab124909) at 1/1000 dilution
Lane 1: 14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 2: 14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 3: 14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged at 10 EU/ng
Lane 4: 14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 5: 14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 6: 14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
Lane 7: 14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3s
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