AB155037

Anti-14-3-3 zeta antibody

Name: Anti-14-3-3 zeta antibody (ab155037) | Abcam
Brand: Abcam Limited
SKU: AB155037

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(4 Publications)

Rabbit Polyclonal 1433Z antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 14-3-3 protein zeta/delta aa 1 to C-terminus.

View Alternative Names

14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (AB155037)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (AB155037)

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft tissue labeling 14-3-3 zeta with ab155037 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (AB155037)

Immunohistochemical analysis of paraffin embedded mouse colon tissue stained for 14 3 3 zeta protein at cytosol with ab155037 at a 1/500 dilution.

Unknown

Western blot - Anti-14-3-3 zeta antibody (AB155037)

12% SDS PAGE

All lanes:

Western blot - Anti-14-3-3 zeta antibody (ab155037) at 1/2000 dilution

Lane 1:

Hep G2 whole cell lysate at 30 µg

Lane 2:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-14-3-3 zeta antibody (AB155037)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-14-3-3 zeta antibody (ab155037) at 1/2000 dilution

Lane 1:

A431 whole cell lysates at 30 µg

Lane 2:

HeLa whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 28 kDa

false

Unknown

Western blot - Anti-14-3-3 zeta antibody (AB155037)

12% SDS PAGE

All lanes:

Western blot - Anti-14-3-3 zeta antibody (ab155037) at 1/1000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 28 kDa,34 kDa

false

Supplier Data

Western blot - Anti-14-3-3 zeta antibody (AB155037)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-14-3-3 zeta antibody (ab155037) at 1/1000 dilution

Lane 1:

Neuro2a whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human 14-3-3 protein zeta/delta aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P63104

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 zeta protein also known as 14-3-3 protein zeta/delta and 1433 protein belongs to the 14-3-3 protein family. These proteins have a molecular weight of around 28-33 kDa. 14-3-3 zeta proteins are expressed across various human tissues including the brain heart and muscles. Mechanically the 14-3-3 zeta protein interacts with different phosphorylated serine and threonine residues on its target proteins. This binding modulates the target proteins' conformation and activity influencing their function in cellular signaling and regulation.

Biological function summary

The 14-3-3 zeta protein plays an important role in several cellular processes. It acts as an adapter in signal transduction pathways and cell cycle control. Additionally the 14-3-3 zeta protein forms complexes with other proteins to regulate apoptosis and stress responses. By binding to various partners this protein influences the distribution and localization of proteins within the cell affecting diverse functions such as metabolism and cell growth.

Pathways

The 14-3-3 zeta protein participates actively in two primary pathways: the MAPK/ERK and PI3K/AKT pathways. Within the MAPK/ERK pathway it influences cellular differentiation and proliferation interacting closely with proteins like Raf and MEK. In the PI3K/AKT pathway 14-3-3 zeta affects cell survival working alongside proteins such as AKT and Bad. These interactions make 14-3-3 zeta a significant player in the regulation of cellular processes.

14-3-3 zeta protein has been connected with neurodegenerative diseases and cancer. Altered expression or function of this protein has been observed in conditions like Alzheimer's disease where it interacts with tau protein and in various cancers where 14-3-3 zeta impacts tumor progression by modulating the function of proteins like p53 and Rb. Understanding these interactions helps in exploring therapeutic strategies for disease intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 14578935, PubMed : 15071501, PubMed : 15644438, PubMed : 16376338, PubMed : 16959763, PubMed : 31024343, PubMed : 9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed : 35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed : 35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed : 16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).

See full target information 14-3-3 protein zeta/delta

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 43:1845-1852 PubMed32236580

2020

NLK interacts with 14‑3‑3ζ to restore the expression of E‑cadherin.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Qingfeng Lin,Tingting Ni,Jiyi Zhao,Feng Lin,Xiangdong Lu,Ye Lv,Shujuan Ren,Zhili Liu,Ting Zhang,Shuyan He,Dong Shen,Weidong Mao

Scientific reports 10:166 PubMed31932597

2020

Comparative Proteomic Analysis in Scar-Free Skin Regeneration in Acomys cahirinus and Scarring Mus musculus.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Hae Yoon,Kun Cho,Timothy J Garrett,Paul Finch,Malcolm Maden

Oxidative medicine and cellular longevity 2018:2025914 PubMed30057671

2018

The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes .

Applications

Unspecified application

Species

Unspecified reactive species

Pan Liu,Xiao Chen,Haizhe Zhou,Liqun Wang,Zaijun Zhang,Xiaohu Ren,Feiqi Zhu,Yi Guo,Xinfeng Huang,Jianjun Liu,Peter S Spencer,Xifei Yang

Frontiers in aging neuroscience 10:72 PubMed29615894

2018

TRPC1 Deletion Causes Striatal Neuronal Cell Apoptosis and Proteomic Alterations in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dian Wang,Haitao Yu,Benhong Xu,Hua Xu,Zaijun Zhang,Xiaohu Ren,Jianhui Yuan,Jianjun Liu,Yi Guo,Peter S Spencer,Xifei Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-14-3-3 zeta antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (AB155037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (AB155037)

Western blot - Anti-14-3-3 zeta antibody (AB155037)

Western blot - Anti-14-3-3 zeta antibody (AB155037)

Western blot - Anti-14-3-3 zeta antibody (AB155037)

Western blot - Anti-14-3-3 zeta antibody (AB155037)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

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Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise