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AB262708

Anti-14-3-3 zeta antibody

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(1 Publication)

Rabbit Polyclonal 1433Z antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 14-3-3 protein zeta/delta.

View Alternative Names

14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

1 Images
Western blot - Anti-14-3-3 zeta antibody (AB262708)
  • WB

Supplier Data

Western blot - Anti-14-3-3 zeta antibody (AB262708)

All lanes:

Western blot - Anti-14-3-3 zeta antibody (ab262708) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 2:

JC (mouse mammary gland adenocarcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human 14-3-3 protein zeta/delta. The exact immunogen used to generate this antibody is proprietary information.

P63104

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 zeta protein also known as 14-3-3 protein zeta/delta and 1433 protein belongs to the 14-3-3 protein family. These proteins have a molecular weight of around 28-33 kDa. 14-3-3 zeta proteins are expressed across various human tissues including the brain heart and muscles. Mechanically the 14-3-3 zeta protein interacts with different phosphorylated serine and threonine residues on its target proteins. This binding modulates the target proteins' conformation and activity influencing their function in cellular signaling and regulation.
Biological function summary

The 14-3-3 zeta protein plays an important role in several cellular processes. It acts as an adapter in signal transduction pathways and cell cycle control. Additionally the 14-3-3 zeta protein forms complexes with other proteins to regulate apoptosis and stress responses. By binding to various partners this protein influences the distribution and localization of proteins within the cell affecting diverse functions such as metabolism and cell growth.

Pathways

The 14-3-3 zeta protein participates actively in two primary pathways: the MAPK/ERK and PI3K/AKT pathways. Within the MAPK/ERK pathway it influences cellular differentiation and proliferation interacting closely with proteins like Raf and MEK. In the PI3K/AKT pathway 14-3-3 zeta affects cell survival working alongside proteins such as AKT and Bad. These interactions make 14-3-3 zeta a significant player in the regulation of cellular processes.

14-3-3 zeta protein has been connected with neurodegenerative diseases and cancer. Altered expression or function of this protein has been observed in conditions like Alzheimer's disease where it interacts with tau protein and in various cancers where 14-3-3 zeta impacts tumor progression by modulating the function of proteins like p53 and Rb. Understanding these interactions helps in exploring therapeutic strategies for disease intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 14578935, PubMed : 15071501, PubMed : 15644438, PubMed : 16376338, PubMed : 16959763, PubMed : 31024343, PubMed : 9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed : 35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed : 35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed : 16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).
See full target information 14-3-3 protein zeta/delta
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed36672654

2023

Impact of Hypermannosylation on the Structure and Functionality of the ER and the Golgi Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Franzka,Svenja Caren Schüler,Takfarinas Kentache,Robert Storm,Andrea Bock,Istvan Katona,Joachim Weis,Katrin Buder,Christoph Kaether,Christian A Hübner
View all publications
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