Anti-14-3-3 zeta antibody [RP23040200]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody [RP23040200] (AB313480)

Immunofluorscent analysis of HeLa cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous 14-3-3 delta/zeta with ab313480 at 2µg/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of 14-3-3 delta/zeta protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of 14-3-3 delta/zeta. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-14-3-3 zeta Antibody [RP23040200] (AB313480)

All lanes:

Western blot - Anti-14-3-3 zeta Antibody [RP23040200] (ab313480) at 1 µg/mL

Lane 1:

SH-SY5Y cell lysate at 30 µg

Lane 2:

MCF7 cell lysate at 30 µg

Lane 3:

A431 cell lysate at 30 µg

Lane 4:

A549 cell lysate at 30 µg

Lane 5:

HeLa cell lysate at 30 µg

Lane 6:

COS7 cell lysate at 30 µg

Lane 7:

NIH/3T3 cell lysate at 30 µg

Lane 8:

PC12 cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Observed band size: 28 kDa

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