AB230919

Anti-15 Lipoxygenase 1 antibody [7H6]

Name: Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919) | Abcam
Brand: Abcam Limited
SKU: AB230919
Price: 605 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal 15 Lipoxygenase 1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein, Transfected cell line samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ALOX15.

View Alternative Names

LOG15, ALOX15, Polyunsaturated fatty acid lipoxygenase ALOX15, 12/15-lipoxygenase, Arachidonate 15-lipoxygenase, Arachidonate omega-6 lipoxygenase, Hepoxilin A3 synthase Alox15, Linoleate 13S-lipoxygenase, 12-LOX, 15-LOX, 15-LOX-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

Formalin-fixed, paraffin-embedded human colon tissue stained for 15 Lipoxygenase 1 using ab230919 at 10 μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

Formalin-fixed, paraffin-embedded human lung tissue stained for 15 Lipoxygenase 1 using ab230919 at 10 μg/ml in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

Intracellular flow cytometric analysis of overexpression plasmid-transfected HEK-293T (human breast adenocarcinoma cell line) cells labeling 15 Lipoxygenase 1 with ab230919 at 1/100 dilution (red) compared withan empty vector control-transfected HEK-293T cell line (blue).

Immunocytochemistry/ Immunofluorescence - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

pCMV6-ENTRY 15 Lipoxygenase 1-transiently transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for 15 Lipoxygenase 1 (green) using ab230919 at 1/100 dilution in ICC/IF.

Supplier Data

Western blot - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

All lanes:

Western blot - Anti-15 Lipoxygenase 1 antibody [7H6] (ab230919) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY 15 Lipoxygenase 1-transfected HEK-293T whole cell lysate at 5 µg

Predicted band size: 75 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7H6

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ALOX15.

P16050

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

15-Lipoxygenase 1 also known as 15-LOX-1 is an enzyme with significant mechanical roles in the human body. Its main function is to catalyze the oxidation of fatty acids especially converting arachidonic acid to 15-hydroperoxyeicosatetraenoic acid (15-HPETE). The protein has other names like arachidonate 15-lipoxygenase or 15-LO. The molecular weight of 15-LOX-1 is approximately 75 kDa. It is mainly expressed in cells of the reticulocyte and tracheobronchial epithelium as well as in macrophages and eosinophils.

Biological function summary

15-Lipoxygenase 1 plays a role in the immune and inflammatory responses. It is involved in lipid mediator biosynthesis producing reactive lipids that influence cell signaling and modulate reactions to inflammation. 15-LOX-1 acts as part of a complex with other enzymes within the lipoxygenase family which includes 5-lipoxygenase and 12-lipoxygenase. This enzyme can also be important for cell maturity and has roles in cell differentiation and maturation particularly in inflamed tissues.

Pathways

Several significant processes involve 15-Lipoxygenase 1 especially its part in the arachidonic acid metabolism and the linoleic acid pathway. In these pathways 15-LOX-1 transforms substances into various lipid signaling molecules like lipoxins and resolvins. These molecules have connections with other proteins like cyclooxygenase-2 (COX-2) and 5-LO which participate in the synthesis of other eicosanoids. The interplay of these pathways and proteins helps in regulating inflammation and resolving it ensuring a balanced biological response to stimuli.

15-Lipoxygenase 1 has associations with conditions including asthma and atherosclerosis. In asthma the enzyme contributes to the inflammatory changes observed in affected airways. Its involvement in this respiratory disorder links it with proteins such as LTC4 synthase that generate pro-inflammatory leukotrienes. In atherosclerosis 15-LOX-1 interacts with oxidized low-density lipoprotein (LDL) in arterial walls which contributes to plaque formation and the associated chronic inflammation. These roles reveal the enzyme's significance in both chronic inflammation and cardiovascular disease settings.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators (PubMed : 17052953, PubMed : 1944593, PubMed : 24282679, PubMed : 25293588, PubMed : 32404334, PubMed : 8334154). It inserts peroxyl groups at C12 or C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing both 12-hydroperoxyeicosatetraenoate/12-HPETE and 15-hydroperoxyeicosatetraenoate/15-HPETE (PubMed : 17052953, PubMed : 1944593, PubMed : 24282679, PubMed : 8334154). It may then act on 12-HPETE to produce hepoxilins, which may show pro-inflammatory properties (By similarity). Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (PubMed : 8334154). May participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs)like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively down-regulate the immune response and have anti-aggregation properties with platelets (PubMed : 32404334). Can convert epoxy fatty acids to hydroperoxy-epoxides derivatives followed by an intramolecular nucleophilic substitution leading to the formation of monocyclic endoperoxides (PubMed : 25293588). Plays an important role during the maintenance of self-tolerance by peroxidizing membrane-bound phosphatidylethanolamine which can then signal the sorting process for clearance of apoptotic cells during inflammation and prevent an autoimmune response. In addition to its role in the immune and inflammatory responses, this enzyme may play a role in epithelial wound healing in the cornea through production of lipoxin A4 (LXA(4)) and docosahexaenoic acid-derived neuroprotectin D1 (NPD1; 10R,17S-HDHA), both lipid autacoids exhibit anti-inflammatory and neuroprotective properties. Furthermore, it may regulate actin polymerization which is crucial for several biological processes such as the phagocytosis of apoptotic cells. It is also implicated in the generation of endogenous ligands for peroxisome proliferator activated receptor (PPAR-gamma), hence modulating macrophage development and function. It may also exert a negative effect on skeletal development by regulating bone mass through this pathway. As well as participates in ER stress and downstream inflammation in adipocytes, pancreatic islets, and liver (By similarity). Finally, it is also involved in the cellular response to IL13/interleukin-13 (PubMed : 21831839).

See full target information ALOX15

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38664011

2024

Calcium-Dependent Regulation of Neuronal Excitability Is Rescued in Fragile X Syndrome by a Tat-Conjugated N-Terminal Fragment of FMRP.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqin Zhan,Hadhimulya Asmara,Paul Pfaffinger,Ray W Turner

View all publications

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Product promise

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Anti-15 Lipoxygenase 1 antibody [7H6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

Flow Cytometry (Intracellular) - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

Immunocytochemistry/ Immunofluorescence - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

Western blot - Anti-15 Lipoxygenase 1 antibody [7H6] (AB230919)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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