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AB113488

Anti-15-LOX-2 antibody

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(1 Publication)

Rabbit Polyclonal 15-LOX-2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ALOX15B aa 1-300.

View Alternative Names

Polyunsaturated fatty acid lipoxygenase ALOX15B, 15-lipoxygenase 2, Arachidonate 15-lipoxygenase B, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, 15-LOX-2, 15-LOX-B, ALOX15B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-LOX-2 antibody (AB113488)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-LOX-2 antibody (AB113488)

ab113488 at 1/100 dilution staining 15-LOX-2 in Formalin-fixed, Paraffin-embedded Human fetal lung tissue by Immunohistochemistry.

Western blot - Anti-15-LOX-2 antibody (AB113488)
  • WB

Unknown

Western blot - Anti-15-LOX-2 antibody (AB113488)

All lanes:

Western blot - Anti-15-LOX-2 antibody (ab113488) at 1/500 dilution

All lanes:

IB8 cell lysate

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ALOX15B aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O15296

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in 200 µL of water
Purification technique
Affinity purification Immunogen
Purification notes
ab113488 is purified by a protein affinity column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

15-LOX-2 also known as 15-lipoxygenase-2 is an enzyme that catalyzes the conversion of arachidonic acid to 15-hydroperoxyicosatetraenoic acid (15-HPETE). Its molecular mass is roughly 76 kDa. 15-LOX-2 is expressed in various tissues with high presence in the prostate lungs and skin. This enzyme is also detected in certain epithelial tissues suggesting its involvement in cellular processes related to skin and prostate health.
Biological function summary

15-LOX-2 plays roles in lipid metabolism by catalyzing specific biochemical reactions. It helps in regulating inflammation through its metabolites. While not part of a larger enzyme complex 15-LOX-2 works alongside other lipoxygenases to manage lipid signaling molecules. Its function further influences cellular differentiation and apoptosis offering an important connection to tissue homeostasis and cellular turnover rates.

Pathways

15-LOX-2 contributes to the arachidonic acid metabolic pathway and eicosanoid signaling pathways. It interacts with cyclooxygenases and other lipoxygenases within these pathways. This interaction affects the balance of pro-inflammatory and anti-inflammatory mediators. The enzyme's activity relates specifically to eicosanoids like leukotrienes and prostaglandins which influence immune responses and vascular functions.

15-LOX-2 shows involvement in prostate cancer and atherosclerosis. Its regulation of inflammatory mediators suggests a role in inflammation-associated pathologies. In prostate cancer altered expression of 15-LOX-2 links to tumor progression interacting with enzymes like COX-2. In atherosclerosis dysregulation of its activity may lead to abnormal lipid metabolism and vessel inflammation demonstrating its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform A. Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Probable) (PubMed : 10542053, PubMed : 10625675, PubMed : 12704195, PubMed : 17493578, PubMed : 18311922, PubMed : 24282679, PubMed : 24497644, PubMed : 32404334, PubMed : 9177185). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (Probable) (PubMed : 10625675, PubMed : 11956198, PubMed : 12704195, PubMed : 17493578, PubMed : 24282679, PubMed : 24497644, PubMed : 9177185). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable) (PubMed : 10542053, PubMed : 27435673). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist (PubMed : 11956198, PubMed : 17493578, PubMed : 18311922). Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates (PubMed : 27145229). Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively down-regulate the immune response and have anti-aggregation properties with platelets (PubMed : 32404334). In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids (PubMed : 27435673). Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation (PubMed : 11839751, PubMed : 12704195). May also regulate cytokine secretion by macrophages and therefore play a role in the immune response (PubMed : 18067895). May also regulate macrophage differentiation into proatherogenic foam cells (PubMed : 22912809).. Isoform B. Does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE.
See full target information ALOX15B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35887187

2022

A Regulatory Axis between Epithelial Splicing Regulatory Proteins and Estrogen Receptor α Modulates the Alternative Transcriptome of Luminal Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jamal Elhasnaoui,Giulio Ferrero,Valentina Miano,Lorenzo Franchitti,Isabella Tarulli,Lucia Coscujuela Tarrero,Santina Cutrupi,Michele De Bortoli
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Product promise

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