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AB217848

Anti-15-PGDH antibody

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(1 Publication)

Rabbit Polyclonal 15-PGDH antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HPGD aa 50-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PGDH1, SDR36C1, HPGD, 15-hydroxyprostaglandin dehydrogenase [NAD(+)], 15-PGDH, Eicosanoid/docosanoid dehydrogenase [NAD(+)], Prostaglandin dehydrogenase 1, Short chain dehydrogenase/reductase family 36C member 1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody (AB217848)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody (AB217848)

Immunohistochemical analysis of paraffin embedded, formalin fixed mouse placenta tissue labeling 15-PGDH using ab217848 at 1/200 dilution, followed by a conjugated secondary for 90 minutes and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human HPGD aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P15428

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Alternative Products

Primary Antibodies

AB187161

Anti-15-PGDH antibody [EPR14332-19]

RabMAb|Recombinant

Western blot - Anti-15-PGDH antibody [EPR14332-19] (AB187161)

  • 0
  • 0
Primary Antibodies

AB232625

Anti-15-PGDH antibody [EPR14332-19] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-15-PGDH antibody [EPR14332-19] - BSA and Azide free (AB232625)

  • 0
  • 0
Primary Antibodies

AB243942

Anti-15-PGDH antibody [EPR14332] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody [EPR14332] - BSA and Azide free (AB243942)

  • 0
  • 0
Primary Antibodies

AB187160

Anti-15-PGDH antibody [EPR14332]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody [EPR14332] (AB187160)

  • 3
  • 2

Complementary Products

Assay Kits

AB273327

15-PGDH Activity Assay Kit (Fluorometric)

Functional Studies - 15-PGDH Activity Assay Kit (Fluorometric) (AB273327)

  • 0
  • 0
Proteins & Peptides

AB99298

Recombinant Human 15-PGDH protein

SDS-PAGE - Recombinant Human 15-PGDH protein (AB99298)

  • 0
  • 0
Primary Antibodies

AB179800

Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (AB179800)

  • 4
  • 3
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB110330

Anti-PDHA1 antibody [9H9AF5]

KO Validated

Western blot - Anti-PDHA1 antibody [9H9AF5] (AB110330)

  • 5
  • 8
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

15-PGDH also known as 15-hydroxyprostaglandin dehydrogenase PGDH or 15-PGDH protein is an enzyme with a molecular mass of approximately 29 kDa. This enzyme catalyzes the oxidation of the 15-hydroxyl group in prostaglandins specifically converting Prostaglandin E2 (PGE2) into its inactive form. Expression occurs in various tissues including the lung placenta and colonic epithelium. Its activity levels can vary depending on the tissue context and physiological conditions marking its importance in local regulation.
Biological function summary

15-PGDH regulates prostaglandin levels which are lipid compounds playing roles in inflammation and homeostasis. This enzyme does not associate with protein complexes but serves a standalone function in metabolic pathways. It maintains a balance between prostaglandin synthesis and degradation ensuring that inflammatory responses do not exceed what is necessary. The enzyme's activity directly reduces available prostaglandins which in turn dampens related signaling pathways.

Pathways

Prostaglandins and this enzyme are part of arachidonic acid metabolism and the Cyclooxygenase (COX) pathway. These pathways play key roles in inflammation and tissue homeostasis. 15-PGDH interacts with other enzymes like COX-2 as it acts downstream to mitigate PGE2 levels generated by COX enzymes. This diminishes the signaling of other elements such as the AMPA receptors involved in neuronal plasticity and pain perception showcasing the enzyme's regulatory scope within biological systems.

15-PGDH plays a role in colon cancer and pulmonary inflammation. Low expression or activity of this enzyme is associated with increased PGE2 levels contributing to cancer progression and chronic inflammation. This dysregulation may involve proteins like COX-2 where the imbalance between synthesis and degradation leads to elevated prostaglandin signaling. Understanding 15-PGDH functions offers insights into therapeutic potentials making inhibitors and potentiators of interest for treatment of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites (PubMed : 10837478, PubMed : 16757471, PubMed : 16828555, PubMed : 21916491, PubMed : 25586183, PubMed : 8086429). Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression (PubMed : 15574495, PubMed : 25586183). Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation (PubMed : 16757471, PubMed : 22844113).
See full target information HPGD
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 34:106-124.e10 PubMed34986329

2022

A purine metabolic checkpoint that prevents autoimmunity and autoinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Svetlana Saveljeva,Gavin W Sewell,Katharina Ramshorn,M Zaeem Cader,James A West,Simon Clare,Lea-Maxie Haag,Rodrigo Pereira de Almeida Rodrigues,Lukas W Unger,Ana Belén Iglesias-Romero,Lorraine M Holland,Christophe Bourges,Muhammad N Md-Ibrahim,James O Jones,Richard S Blumberg,James C Lee,Nicole C Kaneider,Trevor D Lawley,Allan Bradley,Gordon Dougan,Arthur Kaser
View all publications
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Product promise

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