AB187161

Anti-15-PGDH antibody [EPR14332-19]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal 15-PGDH antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

View Alternative Names

PGDH1, SDR36C1, HPGD, 15-hydroxyprostaglandin dehydrogenase [NAD(+)], 15-PGDH, Eicosanoid/docosanoid dehydrogenase [NAD(+)], Prostaglandin dehydrogenase 1, Short chain dehydrogenase/reductase family 36C member 1

2 Images

Supplier Data

Western blot - Anti-15-PGDH antibody [EPR14332-19] (AB187161)

All lanes:

Western blot - Anti-15-PGDH antibody [EPR14332-19] (ab187161) at 1/1000 dilution

Lane 1:

Mouse small intestine lysate at 10 µg

Lane 2:

Rat small intestine lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 25 kDa

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Supplier Data

Western blot - Anti-15-PGDH antibody [EPR14332-19] (AB187161)

Blocking buffer and dilution buffer concentration : 5% NFDM/TBST. This antibody showed low sensitivity in inhouse WB tests, so we suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results.

All lanes:

Western blot - Anti-15-PGDH antibody [EPR14332-19] (ab187161) at 1/1000 dilution

Lane 1:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14332-19

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody showed low sensitivity in inhouse WB tests, so we suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

15-PGDH also known as 15-hydroxyprostaglandin dehydrogenase PGDH or 15-PGDH protein is an enzyme with a molecular mass of approximately 29 kDa. This enzyme catalyzes the oxidation of the 15-hydroxyl group in prostaglandins specifically converting Prostaglandin E2 (PGE2) into its inactive form. Expression occurs in various tissues including the lung placenta and colonic epithelium. Its activity levels can vary depending on the tissue context and physiological conditions marking its importance in local regulation.

Biological function summary

15-PGDH regulates prostaglandin levels which are lipid compounds playing roles in inflammation and homeostasis. This enzyme does not associate with protein complexes but serves a standalone function in metabolic pathways. It maintains a balance between prostaglandin synthesis and degradation ensuring that inflammatory responses do not exceed what is necessary. The enzyme's activity directly reduces available prostaglandins which in turn dampens related signaling pathways.

Pathways

Prostaglandins and this enzyme are part of arachidonic acid metabolism and the Cyclooxygenase (COX) pathway. These pathways play key roles in inflammation and tissue homeostasis. 15-PGDH interacts with other enzymes like COX-2 as it acts downstream to mitigate PGE2 levels generated by COX enzymes. This diminishes the signaling of other elements such as the AMPA receptors involved in neuronal plasticity and pain perception showcasing the enzyme's regulatory scope within biological systems.

15-PGDH plays a role in colon cancer and pulmonary inflammation. Low expression or activity of this enzyme is associated with increased PGE2 levels contributing to cancer progression and chronic inflammation. This dysregulation may involve proteins like COX-2 where the imbalance between synthesis and degradation leads to elevated prostaglandin signaling. Understanding 15-PGDH functions offers insights into therapeutic potentials making inhibitors and potentiators of interest for treatment of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites (PubMed : 10837478, PubMed : 16757471, PubMed : 16828555, PubMed : 21916491, PubMed : 25586183, PubMed : 8086429). Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression (PubMed : 15574495, PubMed : 25586183). Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation (PubMed : 16757471, PubMed : 22844113).

See full target information HPGD

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 14:3149-3160 PubMed40530162

2025

Ubiquitin-specific peptidase 20 affects immune cell infiltration by regulating prostaglandin E2 on intraperitoneal metastasis model of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Hua,Xiukun Ma,Xinyu Zhao,Xiaomeng Wei,Xiaojing Mu,Botao Wang,Xiangfei Yuan

Diabetes, metabolic syndrome and obesity : targets and therapy 16:3223-3234 PubMed37867629

2023

Ginsenoside Rb1 Promotes Hepatic Glycogen Synthesis to Ameliorate T2DM Through 15-PGDH/PGE/EP4 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjie Liang,Wenjing Zhan,Lexun Wang,Weijian Bei,Weixuan Wang

JHEP reports : innovation in hepatology 5:100892 PubMed37942226

2023

Downregulation of 15-PGDH enhances MASH-HCC development via fatty acid-induced T-cell exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Xichen Hu,Tadahito Yasuda,Noriko Yasuda-Yosihara,Atsuko Yonemura,Terumasa Umemoto,Yutaka Nakachi,Kohei Yamashita,Takashi Semba,Kota Arima,Tomoyuki Uchihara,Akiho Nishimura,Luke Bu,Lingfeng Fu,Feng Wei,Jun Zhang,Yilin Tong,Huaitao Wang,Kazuya Iwamoto,Takaichi Fukuda,Hayato Nakagawa,Koji Taniguchi,Yuji Miyamoto,Hideo Baba,Takatsugu Ishimoto

Cancer discovery 12:1984-2005 PubMed35674408

2022

Stress Granules Determine the Development of Obesity-Associated Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Fonteneau,Alexandra Redding,Hannah Hoag-Lee,Edward S Sim,Stefan Heinrich,Matthias M Gaida,Elda Grabocka

JCI insight 6: PubMed33600377

2021

15-PGDH inhibition activates the splenic niche to promote hematopoietic regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Julianne Np Smith,Dawn M Dawson,Kelsey F Christo,Alvin P Jogasuria,Mark J Cameron,Monika I Antczak,Joseph M Ready,Stanton L Gerson,Sanford D Markowitz,Amar B Desai

Cancer science 109:462-470 PubMed29224225

2018

Downregulation of 15-hydroxyprostaglandin dehydrogenase by interleukin-1β from activated macrophages leads to poor prognosis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kota Arima,Yoshihiro Komohara,Luke Bu,Masayo Tsukamoto,Rumi Itoyama,Keisuke Miyake,Tomoyuki Uchihara,Yoko Ogata,Shigeki Nakagawa,Hirohisa Okabe,Katsunori Imai,Daisuke Hashimoto,Akira Chikamoto,Yo-Ichi Yamashita,Hideo Baba,Takatsugu Ishimoto

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-15-PGDH antibody [EPR14332-19]

Western blot - Anti-15-PGDH antibody [EPR14332-19] (AB187161)

Western blot - Anti-15-PGDH antibody [EPR14332-19] (AB187161)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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