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AB254125

Anti-160 kD Neurofilament Medium antibody [3A11] - Neuronal Marker

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(1 Publication)

Mouse Monoclonal NFM antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat Nefm.

View Alternative Names

Nef3, Nfm, Nefm, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [3A11] - Neuronal Marker (AB254125)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [3A11] - Neuronal Marker (AB254125)

Cultured rat neurons stained for 160 kD Neurofilament Medium (green, in mature neurons) using ab254125 at 1/100 dilution in ICC/IF.

Western blot - Anti-160 kD Neurofilament Medium antibody [3A11] - Neuronal Marker (AB254125)
  • WB

Supplier Data

Western blot - Anti-160 kD Neurofilament Medium antibody [3A11] - Neuronal Marker (AB254125)

Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody [3A11] - Neuronal Marker (ab254125) at 1/1000 dilution

All lanes:

Rat cortex lysate

Predicted band size: 102 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A11

Isotype

IgG1

Carrier free

No

Reacts with

Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Rat Nefm. The exact immunogen used to generate this antibody is proprietary information.

P12839

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Unpurified, concentrated tissue culture supernatant.
Storage buffer
Preservative: 0.065% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 160 kD Neurofilament Medium also known as NEFM NF-M or NEF-M belongs to the family of neurofilament proteins. This protein is an important structural component of neurons particularly in the axonal cytoskeleton. Neurofilament Medium has a molecular mass of around 160 kilodaltons (kD). It shows high levels of expression in neuronal tissues contributing to the maintenance of axonal caliber. Commonly research recognizes it alongside other neurofilament proteins emphasizing its role in providing structural integrity to neurons.
Biological function summary

Neurofilament Medium participates in the formation of a stable network of neurofilaments within neurons. Acting as a part of the intermediate filament protein family it forms a complex with other neurofilaments such as NF-L and NF-H. This complex supports neuron structure and plays an important role in axonal transport. The heteropolymeric nature of neurofilaments contributes to their mechanical stability facilitating essential neuronal functions by aligning neurofilaments along the axon.

Pathways

Neurofilament Medium integrates into the cytoskeletal arrangement pathways that govern axonal transport and stability. It plays a significant role in the neuronal transport pathway associating with other proteins such as dynein and kinesin responsible for the motor functions along axons. Also it relates to the MAP kinase pathway where phosphorylation events modulate its assembly and disassembly dynamics in response to cellular signals.

Neurofilament Medium closely relates to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Aberrant phosphorylation or aggregation of NEFM can disrupt neuronal function leading to pathology. In ALS the abnormal accumulation of neurofilaments correlates with motor neuron degeneration. In Alzheimer's its interaction with tau protein poses significant interest as altered states of either could exacerbate neurofibrillary tangles worsening cognitive decline.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information Nefm
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9529 PubMed39532843

2024

Nuclear localization of MTHFD2 is required for correct mitosis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Pardo-Lorente,Anestis Gkanogiannis,Luca Cozzuto,Antoni Gañez Zapater,Lorena Espinar,Ritobrata Ghose,Jacqueline Severino,Laura García-López,Rabia Gül Aydin,Laura Martin,Maria Victoria Neguembor,Evangelia Darai,Maria Pia Cosma,Laura Batlle-Morera,Julia Ponomarenko,Sara Sdelci
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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