Chicken Polyclonal NFM antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nefm.
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- eLife 10:2021Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrent A Wilkerson,Heather L Zebroski,Connor R Finkbeiner,Alex D Chitsazan,Kylie E Beach,Nilasha Sen,Renee C Zhang,Olivia Bermingham-McDonoghPubMed 34003106
- Brain sciences 10:2020Flumazenil-Insensitive Benzodiazepine Effects in Recombinant αβ and Neuronal GABA Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing-Jing Lian et. al.PubMed 32150806
- Cellular and molecular gastroenterology and hepatology 9:465-4832019Mu and Delta Opioid Receptors Are Coexpressed and Functionally Interact in the Enteric Nervous System of the Mouse Colon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJesse J DiCello,Simona E Carbone,Ayame Saito,Pradeep Rajasekhar,Rhian A Ceredig,Vi Pham,Celine Valant,Arthur Christopoulos,Nicholas A Veldhuis,Meritxell Canals,Dominique Massotte,Daniel P PoolePubMed 31759144
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Nefm. The exact immunogen used to generate this antibody is proprietary information. Database link P12839
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Mouse | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes Use 2% paraformaldehyde-fixed cells. |
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Target data
Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Alternative names
Nef3, Nfm, Nefm, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
Recommended products
Chicken Polyclonal NFM antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nefm.
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Nefm. The exact immunogen used to generate this antibody is proprietary information. Database link P12839
- Purity
- IgY fraction
- Concentration
- Purification notes
Immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in buffer and then filter-sterilized.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 160 kD Neurofilament Medium also known as NEFM NF-M or NEF-M belongs to the family of neurofilament proteins. This protein is an important structural component of neurons particularly in the axonal cytoskeleton. Neurofilament Medium has a molecular mass of around 160 kilodaltons (kD). It shows high levels of expression in neuronal tissues contributing to the maintenance of axonal caliber. Commonly research recognizes it alongside other neurofilament proteins emphasizing its role in providing structural integrity to neurons.
- Biological function summary
Neurofilament Medium participates in the formation of a stable network of neurofilaments within neurons. Acting as a part of the intermediate filament protein family it forms a complex with other neurofilaments such as NF-L and NF-H. This complex supports neuron structure and plays an important role in axonal transport. The heteropolymeric nature of neurofilaments contributes to their mechanical stability facilitating essential neuronal functions by aligning neurofilaments along the axon.
- Pathways
Neurofilament Medium integrates into the cytoskeletal arrangement pathways that govern axonal transport and stability. It plays a significant role in the neuronal transport pathway associating with other proteins such as dynein and kinesin responsible for the motor functions along axons. Also it relates to the MAP kinase pathway where phosphorylation events modulate its assembly and disassembly dynamics in response to cellular signals.
- Associated diseases and disorders
Neurofilament Medium closely relates to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Aberrant phosphorylation or aggregation of NEFM can disrupt neuronal function leading to pathology. In ALS the abnormal accumulation of neurofilaments correlates with motor neuron degeneration. In Alzheimer's its interaction with tau protein poses significant interest as altered states of either could exacerbate neurofibrillary tangles worsening cognitive decline.
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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We do not recommend this combination. It is not covered by our product promise.
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1 product image
Picture courtesy of Dr. Gerry Shaw, University of FloridaImmunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody (ab134458)
Immunofluorescent analysis of dissociated culture of mouse cortical neurons labelling 160 kD Neurofilament Medium with ab134458 at 1/2000 dilution (green staining). Neurites are stained in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence at 1/5000 dilution. Blue nuclei demonstrate DAPI DNA staining.
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