AB134458

Anti-160 kD Neurofilament Medium antibody

Name: Anti-160 kD Neurofilament Medium antibody (ab134458) | Abcam
Brand: Abcam Limited
SKU: AB134458

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(6 Publications)

Chicken Polyclonal NFM antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nefm.

View Alternative Names

Nef3, Nfm, Nefm, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody (AB134458)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody (AB134458)

Immunofluorescent analysis of dissociated culture of mouse cortical neurons labelling 160 kD Neurofilament Medium with ab134458 at 1/2000 dilution (green staining). Neurites are stained in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence at 1/5000 dilution. Blue nuclei demonstrate DAPI DNA staining.

Picture courtesy of Dr. Gerry Shaw, University of Florida

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Rat Nefm. The exact immunogen used to generate this antibody is proprietary information.

P12839

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/2000", "ICCIF-species-notes": "<p>Use 2% paraformaldehyde-fixed cells.</p>" } } }

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Product details

Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

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Properties and storage information

Form

Liquid

Purity

IgY fraction

Purification notes

Immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in buffer and then filter-sterilized.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 160 kD Neurofilament Medium also known as NEFM NF-M or NEF-M belongs to the family of neurofilament proteins. This protein is an important structural component of neurons particularly in the axonal cytoskeleton. Neurofilament Medium has a molecular mass of around 160 kilodaltons (kD). It shows high levels of expression in neuronal tissues contributing to the maintenance of axonal caliber. Commonly research recognizes it alongside other neurofilament proteins emphasizing its role in providing structural integrity to neurons.

Biological function summary

Neurofilament Medium participates in the formation of a stable network of neurofilaments within neurons. Acting as a part of the intermediate filament protein family it forms a complex with other neurofilaments such as NF-L and NF-H. This complex supports neuron structure and plays an important role in axonal transport. The heteropolymeric nature of neurofilaments contributes to their mechanical stability facilitating essential neuronal functions by aligning neurofilaments along the axon.

Pathways

Neurofilament Medium integrates into the cytoskeletal arrangement pathways that govern axonal transport and stability. It plays a significant role in the neuronal transport pathway associating with other proteins such as dynein and kinesin responsible for the motor functions along axons. Also it relates to the MAP kinase pathway where phosphorylation events modulate its assembly and disassembly dynamics in response to cellular signals.

Neurofilament Medium closely relates to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Aberrant phosphorylation or aggregation of NEFM can disrupt neuronal function leading to pathology. In ALS the abnormal accumulation of neurofilaments correlates with motor neuron degeneration. In Alzheimer's its interaction with tau protein poses significant interest as altered states of either could exacerbate neurofibrillary tangles worsening cognitive decline.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information Nefm

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1290128 PubMed38384299

2024

Neuroprotective effects of a novel peptide through the Rho-integrin-Tie2 and PI3K/Akt pathways in experimental autoimmune encephalomyelitis model.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhou,Han Qu,Xiao-Xiao Fu,Miao-Miao Xu,Qiang Li,Yuan Jiang,Shu Han

eLife 13: PubMed38192199

2024

A translational MRI approach to validate acute axonal damage detection as an early event in multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Cerdán Cerdá,Nicola Toschi,Constantina A Treaba,Valeria Barletta,Elena Herranz,Ambica Mehndiratta,Jose A Gomez-Sanchez,Caterina Mainero,Silvia De Santis

The EMBO journal 41:e108780 PubMed35815410

2022

Schwann cell precursors represent a neural crest-like state with biased multipotency.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Eleni Kastriti,Louis Faure,Dorothea Von Ahsen,Thibault Gerald Bouderlique,Johan Boström,Tatiana Solovieva,Cameron Jackson,Marianne Bronner,Dies Meijer,Saida Hadjab,Francois Lallemend,Alek Erickson,Marketa Kaucka,Viacheslav Dyachuk,Thomas Perlmann,Laura Lahti,Jan Krivanek,Jean-Francois Brunet,Kaj Fried,Igor Adameyko

eLife 10: PubMed34003106

2021

Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris.

Applications

Unspecified application

Species

Unspecified reactive species

Brent A Wilkerson,Heather L Zebroski,Connor R Finkbeiner,Alex D Chitsazan,Kylie E Beach,Nilasha Sen,Renee C Zhang,Olivia Bermingham-McDonogh

Brain sciences 10: PubMed32150806

2020

Flumazenil-Insensitive Benzodiazepine Effects in Recombinant αβ and Neuronal GABA Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Jing Lian,Yan-Qing Cao,Yu-Lei Li,Gang Yu,Rui-Bin Su

Cellular and molecular gastroenterology and hepatology 9:465-483 PubMed31759144

2019

Mu and Delta Opioid Receptors Are Coexpressed and Functionally Interact in the Enteric Nervous System of the Mouse Colon.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse J DiCello,Simona E Carbone,Ayame Saito,Pradeep Rajasekhar,Rhian A Ceredig,Vi Pham,Celine Valant,Arthur Christopoulos,Nicholas A Veldhuis,Meritxell Canals,Dominique Massotte,Daniel P Poole

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-160 kD Neurofilament Medium antibody

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody (AB134458)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

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Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise