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AB72998

Anti-160 kD Neurofilament Medium antibody

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(3 Publications)

Chicken Polyclonal NFM antibody. Suitable for WB and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nefm.

View Alternative Names

Nef3, Nfm, Nefm, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

1 Images
Western blot - Anti-160 kD Neurofilament Medium antibody (AB72998)
  • WB

Unknown

Western blot - Anti-160 kD Neurofilament Medium antibody (AB72998)

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody (ab72998) at 1/5000 dilution

All lanes:

rat cerebral cortex lysate

Predicted band size: 102 kDa

Observed band size: 145 kDa

false

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Rat Nefm. The exact immunogen used to generate this antibody is proprietary information.

P12839

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Primary Antibodies

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgY fraction
Storage buffer
Preservative: 0.065% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 160 kD Neurofilament Medium also known as NEFM NF-M or NEF-M belongs to the family of neurofilament proteins. This protein is an important structural component of neurons particularly in the axonal cytoskeleton. Neurofilament Medium has a molecular mass of around 160 kilodaltons (kD). It shows high levels of expression in neuronal tissues contributing to the maintenance of axonal caliber. Commonly research recognizes it alongside other neurofilament proteins emphasizing its role in providing structural integrity to neurons.
Biological function summary

Neurofilament Medium participates in the formation of a stable network of neurofilaments within neurons. Acting as a part of the intermediate filament protein family it forms a complex with other neurofilaments such as NF-L and NF-H. This complex supports neuron structure and plays an important role in axonal transport. The heteropolymeric nature of neurofilaments contributes to their mechanical stability facilitating essential neuronal functions by aligning neurofilaments along the axon.

Pathways

Neurofilament Medium integrates into the cytoskeletal arrangement pathways that govern axonal transport and stability. It plays a significant role in the neuronal transport pathway associating with other proteins such as dynein and kinesin responsible for the motor functions along axons. Also it relates to the MAP kinase pathway where phosphorylation events modulate its assembly and disassembly dynamics in response to cellular signals.

Neurofilament Medium closely relates to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Aberrant phosphorylation or aggregation of NEFM can disrupt neuronal function leading to pathology. In ALS the abnormal accumulation of neurofilaments correlates with motor neuron degeneration. In Alzheimer's its interaction with tau protein poses significant interest as altered states of either could exacerbate neurofibrillary tangles worsening cognitive decline.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information Nefm
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 15: PubMed36904077

2023

Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lyvianne Decourtye-Espiard,Maud Clemessy,Patricia Leneuve,Erik Mire,Tatiana Ledent,Yves Le Bouc,Laurent Kappeler

PloS one 13:e0193196 PubMed29466413

2018

Impact of insulin on primary arcuate neurons culture is dependent on early-postnatal nutritional status and neuronal subpopulation.

Applications

Unspecified application

Species

Unspecified reactive species

Lyvianne Decourtye,Maud Clemessy,Erik Mire,Tatiana Ledent,Laurence Périn,Iain C Robinson,Yves Le Bouc,Laurent Kappeler

PloS one 12:e0170083 PubMed28076448

2017

IGF-1 Induces GHRH Neuronal Axon Elongation during Early Postnatal Life in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lyvianne Decourtye,Erik Mire,Maud Clemessy,Victor Heurtier,Tatiana Ledent,Iain C Robinson,Patrice Mollard,Jacques Epelbaum,Michael J Meaney,Sonia Garel,Yves Le Bouc,Laurent Kappeler
View all publications
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Product promise

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