AB9034

Anti-160 kD Neurofilament Medium antibody

Name: Anti-160 kD Neurofilament Medium antibody (ab9034) | Abcam
Brand: Abcam Limited
SKU: AB9034
Price: 585 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(20 Publications)

Rabbit Polyclonal NFM antibody. Suitable for WB, IHC-FrFl and reacts with Mouse, Rat, Pig samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nefm.

View Alternative Names

Nef3, Nfm, Nefm, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

2 Images

Immunohistochemistry - Free Floating - Anti-160 kD Neurofilament Medium antibody (AB9034)

IHC-FrFl

Supplier Data

Immunohistochemistry - Free Floating - Anti-160 kD Neurofilament Medium antibody (AB9034)

Immunohistochemistry - Free Floating analysis of rat cerebellum section labeling 160 kD Neurofilament Medium with ab9034 at 1/2000 diution (red) and and costained with mouse mAb to GAP43 at 1/2000 diution (green). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. ab9034 strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regions in the molecular layer.

Supplier Data

Western blot - Anti-160 kD Neurofilament Medium antibody (AB9034)

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody (ab9034) at 1/2000 dilution

Lane 1:

Protein standard

Lane 2:

Rat brain tissue lysate

Lane 3:

Rat spinal cord tissue lysate

Lane 4:

Mouse brain tissue lysate

Lane 5:

Mouse spinal cord tissue lysate

Lane 6:

Pig brain tissue lysate

Lane 7:

Pig spinal cord tissue lysate

Predicted band size: 102 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Pig

Applications

IHC-FrFl, WB

applications

Immunogen

Recombinant Fragment Protein within Rat Nefm. The exact immunogen used to generate this antibody is proprietary information.

P12839

Specificity

Recognizes the evolutionarily conserved extreme C-terminal region of the medium neurofilament subunit (~145-170 kDa).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFrFl" : {"fullname" : "Immunohistochemistry - Free Floating", "shortname":"IHC-FrFl"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCFrFl-species-checked": "testedAndGuaranteed", "IHCFrFl-species-dilution-info": "1/2000", "IHCFrFl-species-notes": "" }, "Bird": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFrFl-species-checked": "predicted", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFrFl-species-checked": "predicted", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" }, "Lizard": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFrFl-species-checked": "predicted", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.1% Sodium azide Constituents: 70% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 160 kD Neurofilament Medium also known as NEFM NF-M or NEF-M belongs to the family of neurofilament proteins. This protein is an important structural component of neurons particularly in the axonal cytoskeleton. Neurofilament Medium has a molecular mass of around 160 kilodaltons (kD). It shows high levels of expression in neuronal tissues contributing to the maintenance of axonal caliber. Commonly research recognizes it alongside other neurofilament proteins emphasizing its role in providing structural integrity to neurons.

Biological function summary

Neurofilament Medium participates in the formation of a stable network of neurofilaments within neurons. Acting as a part of the intermediate filament protein family it forms a complex with other neurofilaments such as NF-L and NF-H. This complex supports neuron structure and plays an important role in axonal transport. The heteropolymeric nature of neurofilaments contributes to their mechanical stability facilitating essential neuronal functions by aligning neurofilaments along the axon.

Pathways

Neurofilament Medium integrates into the cytoskeletal arrangement pathways that govern axonal transport and stability. It plays a significant role in the neuronal transport pathway associating with other proteins such as dynein and kinesin responsible for the motor functions along axons. Also it relates to the MAP kinase pathway where phosphorylation events modulate its assembly and disassembly dynamics in response to cellular signals.

Neurofilament Medium closely relates to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Aberrant phosphorylation or aggregation of NEFM can disrupt neuronal function leading to pathology. In ALS the abnormal accumulation of neurofilaments correlates with motor neuron degeneration. In Alzheimer's its interaction with tau protein poses significant interest as altered states of either could exacerbate neurofibrillary tangles worsening cognitive decline.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (PubMed : 22723690).

See full target information Nefm

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroanatomy 17:1235047 PubMed37603782

2023

Early cellular and synaptic changes in dopaminoceptive forebrain regions of juvenile mice following gestational exposure to valproate.

Applications

Unspecified application

Species

Unspecified reactive species

Cintia Klaudia Finszter,Róbert Kemecsei,Gergely Zachar,Sophie Holtkamp,Diego Echevarría,István Adorján,Ágota Ádám,András Csillag

Cell reports 42:112118 PubMed36774552

2023

Regional and cell-type-specific afferent and efferent projections of the mouse claustrum.

Applications

Unspecified application

Species

Unspecified reactive species

Quanxin Wang,Yun Wang,Hsien-Chi Kuo,Peng Xie,Xiuli Kuang,Karla E Hirokawa,Maitham Naeemi,Shenqin Yao,Matt Mallory,Ben Ouellette,Phil Lesnar,Yaoyao Li,Min Ye,Chao Chen,Wei Xiong,Leila Ahmadinia,Laila El-Hifnawi,Ali Cetin,Staci A Sorensen,Julie A Harris,Hongkui Zeng,Christof Koch

Medicina (Kaunas, Lithuania) 59: PubMed36837509

2023

Distributions of Platelet-Derived Growth Factor Receptor-α Positive Cells and Interstitial Cells of Cajal in the Colon of Rats with Diabetes Mellitus Type 2.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandra Ivana Veličkov,Branka Djordjević,Milica Lazarević,Asen Veselin Veličkov,Vladimir Petrović,Marko Jović,Tijana Denčić,Goran Radenković

NeuroImage. Clinical 37:103318 PubMed36630864

2023

Microstructural mapping of dentate gyrus pathology in Alzheimer's disease: A 16.4 Tesla MRI study.

Applications

Unspecified application

Species

Unspecified reactive species

Nien-Chu Shih,Nyoman D Kurniawan,Ryan P Cabeen,Laura Korobkova,Ellen Wong,Helena C Chui,Kristi A Clark,Carol A Miller,Debra Hawes,Kymry T Jones,Farshid Sepehrband

Development (Cambridge, England) 149: PubMed34878101

2022

Stabilization of β-catenin promotes melanocyte specification at the expense of the Schwann cell lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Colombo,Valérie Petit,Roselyne Y Wagner,Delphine Champeval,Ichiro Yajima,Franck Gesbert,Zackie Aktary,Irwin Davidson,Véronique Delmas,Lionel Larue

Polymers 12: PubMed33260420

2020

Aragonite-Polylysine: Neuro-Regenerative Scaffolds with Diverse Effects on Astrogliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tzachy Morad,Roni Mina Hendler,Eyal Canji,Orly Eva Weiss,Guy Sion,Refael Minnes,Ania Hava Grushchenko Polaq,Ido Merfeld,Zvy Dubinsky,Elimelech Nesher,Danny Baranes

Annals of neurology 88:1023-1027 PubMed32740999

2020

Effects of IgLON5 Antibodies on Neuronal Cytoskeleton: A Link between Autoimmunity and Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jon Landa,Carles Gaig,Jesús Plagumà,Albert Saiz,Ana Antonell,Raquel Sanchez-Valle,Josep Dalmau,Francesc Graus,Lidia Sabater

Frontiers in neuroanatomy 14:29 PubMed32581730

2020

Gestational Exposure to Sodium Valproate Disrupts Fasciculation of the Mesotelencephalic Dopaminergic Tract, With a Selective Reduction of Dopaminergic Output From the Ventral Tegmental Area.

Applications

Unspecified application

Species

Unspecified reactive species

Ágota Ádám,Róbert Kemecsei,Verónica Company,Raquel Murcia-Ramón,Iris Juarez,László I Gerecsei,Gergely Zachar,Diego Echevarría,Eduardo Puelles,Salvador Martínez,András Csillag

Molecular neurobiology 57:949-963 PubMed31630330

2019

Transcriptional Profiling of Individual Airway Projecting Vagal Sensory Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Stuart B Mazzone,Luyi Tian,Aung Aung Kywe Moe,Matthew W Trewella,Matthew E Ritchie,Alice E McGovern

The American journal of pathology 189:1375-1385 PubMed31230667

2019

Nitrosative Stress Is Associated with Dopaminergic Dysfunction in the HIV-1 Transgenic Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Swati Shah,Dragan Maric,Frank Denaro,Wael Ibrahim,Ronald Mason,Ashutosh Kumar,Dima A Hammoud,William Reid

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-160 kD Neurofilament Medium antibody

Immunohistochemistry - Free Floating - Anti-160 kD Neurofilament Medium antibody (AB9034)

Western blot - Anti-160 kD Neurofilament Medium antibody (AB9034)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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