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AB215528

Anti-17-beta-Estradiol antibody [EPPTX-R2]

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(3 Publications)

Rabbit Recombinant Monoclonal 17-beta-Estradiol antibody. Suitable for cELISA and reacts with Chemical samples. Cited in 3 publications.
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Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] (AB215528)
  • cELISA

Supplier Data

Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] (AB215528)

ab215528 is specific to 17β-Estradiol and the percent cross reactivity with other compounds is demonstrated in the table.

The data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] (AB215528)
  • cELISA

Supplier Data

Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] (AB215528)

Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of 17β-Estradiol (0, 1, 2, 4, 8, 16, 32 pg/ml) was added (75 μl). Then 3-5 ng/ml of ab215528 (25 μl) was added followed by incubation by shaking for 60 minutes at room temperature. Then 25 μl of HRP-conjugated 17β-Estradiol was added and incubated for 60 minutes at room temperature. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 30 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

  • Carrier free

    Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPPTX-R2

Isotype

IgG

Carrier free

No

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

AB224259

Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free

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Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (AB224259)

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Primary Antibodies

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Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] (AB215527)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p>Use 3-5 ng/ml.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

17-beta-Estradiol also known simply as estradiol or beta-estradiol is a steroid hormone and a member of the estrogen family. It has a molecular mass of approximately 272.39 g/mol. This hormone is synthesized mainly in the ovaries and to a lesser extent in the adrenal glands and the male testes. In cellular mechanics 17-beta-Estradiol functions by entering cells and binding to specific nuclear estrogen receptors alpha (ERα) and beta (ERβ). This binding triggers changes in the cell influencing gene expression and cellular activity.
Biological function summary

17-beta-Estradiol exerts significant roles in reproductive systems such as regulating the menstrual cycle and maintaining pregnancy. It is part of the steroid hormone pathway working closely with proteins like progesterone receptors. Besides its reproductive functions 17-beta-Estradiol impacts bone density cardiovascular health and brain function highlighting its systemic importance. It does not form part of a protein complex but interacts with multiple transcription factors to execute its functions.

Pathways

Hormone synthesis and regulation pathways prominently feature 17-beta-Estradiol. It takes part in the hypothalamic-pituitary-gonadal axis where it collaborates with luteinizing hormone (LH) and follicle-stimulating hormone (FSH) to regulate reproductive functions. Additionally 17-beta-Estradiol contributes to the bone remodeling pathway by affecting osteoclast and osteoblast activity together with proteins like RANKL and OPG.

17-beta-Estradiol has been strongly linked to conditions such as breast cancer and osteoporosis. It influences breast cancer progression by interacting with various receptor proteins including HER2 and progesterone receptor leading to proliferation in certain cancerous cells. In osteoporosis insufficient levels of 17-beta-Estradiol lead to increased bone resorption and decreased bone formation linking it to proteins like collagen and osteocalcin involved in bone structure and strength maintenance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information 17-beta-Estradiol
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 12:705012 PubMed34335537

2021

Light-Stress Response Mediated by the Transcription Factor Mga2 in the Yeast .

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Camponeschi,Arianna Montanari,Marzia Beccaccioli,Massimo Reverberi,Cristina Mazzoni,Michele M Bianchi

The Journal of reproduction and development 66:453-458 PubMed32595196

2020

Artificial lactation by exogenous hormone treatment in non-pregnant sows.

Applications

Unspecified application

Species

Unspecified reactive species

Michiko Noguchi,Takehito Suzuki,Reiichiro Sato,Yosuke Sasaki,Kazuyuki Kaneko

The Journal of veterinary medical science 82:590-597 PubMed32173693

2020

Day of prostaglandin F administration after natural ovulation affects the interval to ovulation, the type of ovulated follicle, and the failure to induce ovulation in cows.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuyuki Kaneko,Kanumporn Mungthong,Michiko Noguchi
View all publications
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Product promise

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