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AB224259

Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal 17-beta-Estradiol antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.
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Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (AB224259)
  • cELISA

Supplier Data

Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (AB224259)

ab224259 is specific to 17β-Estradiol and the percent cross reactivity with other compounds is demonstrated in the table.

The data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (AB224259)
  • cELISA

Supplier Data

Competitive ELISA - Anti-17-beta-Estradiol antibody [EPPTX-R2] - BSA and Azide free (AB224259)

This ELISA data used the same anti-17-beta-Estradiol antibody clone [EPPTX-R2] in a different buffer formulation (cat# ab215528).

Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of 17β-Estradiol (0, 1, 2, 4, 8, 16, 32 pg/ml) was added (75 μl). Then 3-5 ng/ml of ab215528 (25 μl) was added followed by incubation by shaking for 60 minutes at room temperature. Then 25 μl of HRP-conjugated 17β-Estradiol was added and incubated for 60 minutes at room temperature. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 30 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPPTX-R2

Isotype

IgG

Carrier free

Yes

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>" } } }
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Product details

ab224259 is the carrier-free version of ab215528.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

17-beta-Estradiol also known simply as estradiol or beta-estradiol is a steroid hormone and a member of the estrogen family. It has a molecular mass of approximately 272.39 g/mol. This hormone is synthesized mainly in the ovaries and to a lesser extent in the adrenal glands and the male testes. In cellular mechanics 17-beta-Estradiol functions by entering cells and binding to specific nuclear estrogen receptors alpha (ERα) and beta (ERβ). This binding triggers changes in the cell influencing gene expression and cellular activity.
Biological function summary

17-beta-Estradiol exerts significant roles in reproductive systems such as regulating the menstrual cycle and maintaining pregnancy. It is part of the steroid hormone pathway working closely with proteins like progesterone receptors. Besides its reproductive functions 17-beta-Estradiol impacts bone density cardiovascular health and brain function highlighting its systemic importance. It does not form part of a protein complex but interacts with multiple transcription factors to execute its functions.

Pathways

Hormone synthesis and regulation pathways prominently feature 17-beta-Estradiol. It takes part in the hypothalamic-pituitary-gonadal axis where it collaborates with luteinizing hormone (LH) and follicle-stimulating hormone (FSH) to regulate reproductive functions. Additionally 17-beta-Estradiol contributes to the bone remodeling pathway by affecting osteoclast and osteoblast activity together with proteins like RANKL and OPG.

17-beta-Estradiol has been strongly linked to conditions such as breast cancer and osteoporosis. It influences breast cancer progression by interacting with various receptor proteins including HER2 and progesterone receptor leading to proliferation in certain cancerous cells. In osteoporosis insufficient levels of 17-beta-Estradiol lead to increased bone resorption and decreased bone formation linking it to proteins like collagen and osteocalcin involved in bone structure and strength maintenance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information 17-beta-Estradiol
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6446 PubMed37833281

2023

Rewiring of the promoter-enhancer interactome and regulatory landscape in glioblastoma orchestrates gene expression underlying neurogliomal synaptic communication.

Applications

Unspecified application

Species

Unspecified reactive species

Chaitali Chakraborty,Itzel Nissen,Craig A Vincent,Anna-Carin Hägglund,Andreas Hörnblad,Silvia Remeseiro
View all publications
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Product promise

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