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Rabbit Recombinant Monoclonal TAU antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Transfected cell line - Human, Rat, Transfected cell lysate - Human, Recombinant fragment - Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (AB314889), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-1N Tau antibody [EPR25206-82] (AB314889), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (AB314889), expandable thumbnail
  • Western blot - Anti-1N Tau antibody [EPR25206-82] (AB314889), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (AB314889), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFIP
Human
Tested
Tested
Expected
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Tested
Not recommended
Not recommended
Recombinant fragment - Human
Not recommended
Tested
Not recommended
Not recommended
Transfected cell line - Human
Tested
Not recommended
Tested
Not recommended
Transfected cell lysate - Human
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Transfected cell line - Human

Dilution info
1/20000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Transfected cell lysate - Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

-

Species

Rat

Dilution info
1/1000
Notes

-

Species

Transfected cell lysate - Human

Dilution info
1/1000
Notes

-

Species

Recombinant fragment - Human

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Mouse, Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Human

Dilution info
1/50
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Transfected cell lysate - Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse, Transfected cell line - Human, Transfected cell lysate - Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Targets

MAPT

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TAU antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Transfected cell line - Human, Rat, Transfected cell lysate - Human, Recombinant fragment - Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR25206-82

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

1N Tau also known as human Tau protein plays a role in stabilizing microtubules in neurons. It is a low molecular weight protein with a mass of around 45 kDa. The expression of 1N Tau occurs mostly in the central nervous system but is also present in smaller quantities in peripheral nervous tissues. This protein binds directly to microtubules through its repeat domains which forms a tight and stable structure necessary for proper neuronal function.

Biological function summary

1N Tau is critical in the assembly and stabilization of the neuronal cytoskeleton. It normally functions as part of a larger microtubule-associated protein complex. By promoting microtubule polymerization it helps maintain the axonal transport system necessary for neuronal communication. The functional activity of 1N Tau can be controlled by phosphorylation a process that can modulate its binding affinity to microtubules.

Pathways

The cellular processes involving 1N Tau often intersect with important signaling cascades such as the MAPK/ERK and PI3K/Akt pathways. The alterations in phosphorylation states regulate its interaction with other microtubule-associated proteins like MAP2 and MAP4. This regulation ensures the stability of the neuronal cytoskeleton and influences processes like cell growth and survival impacting overall neuronal health and function.

Associated diseases and disorders

Abnormal 1N Tau phosphorylation and aggregation are closely associated with neurodegenerative diseases such as Alzheimer's disease and frontotemporal dementia. These conditions often show the presence of paired helical filaments formed by hyperphosphorylated 1N Tau. Interaction with amyloid precursor protein (APP) in Alzheimer's disease suggests a link between Tau aggregation and amyloid beta pathology which contributes to neuronal dysfunction and cognitive decline.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (ab314889), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (ab314889)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling 1N Tau with ab314889 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cerebrum.The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-1N Tau antibody [EPR25206-82] (ab314889), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-1N Tau antibody [EPR25206-82] (ab314889)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling 1N Tau with ab314889 at 1/50 (10.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing positive staining in 293T cells transfected with a human Tau 1N3R and Tau 1N4R expression vector containing a myc-tag, showing no staining in Tau 0N4R/0N3R/2N3R/2N4R(WT) and empty expression vector containing a myc-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (ab314889), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (ab314889)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling 1N Tau with ab314889 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human spleen. The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889), expandable thumbnail

    Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

    In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution, Anti-Tau antibody [EPR22524-95] (Anti-Tau antibody [EPR22524-95] ab254256) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human Tau 0N3R expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human Tau 0N4R expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a human Tau 1N3R expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 5: 293T cells transfected with a human Tau 1N4R expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 6: 293T cells transfected with a human Tau 2N3R expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 7: 293T cells transfected with a human Tau 2N4R expression vector containing a His-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 37-75 kDa, 15 kDa

    Exposure time: 6s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (ab314889), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (ab314889)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human Tau 1N3R expression vector containing a his tag. (B) HEK-293T transfected with a human Tau 1N4R expression vector containing a his tag. (C) HEK-293T transfected with a human Tau 0N3R expression vector containing a his tag. (D) HEK-293T transfected with a human Tau 2N3R expression vector containing a his tag. (E) HEK-293T transfected with a human Tau 0N4R expression vector containing a his tag. (F) HEK-293T transfected with a human Tau 2N4R expression vector containing a his tag. (G) HEK-293T cells transfected with empty vector containing a his tag. tissue labeling 1N Tau with ab314889 at 1/20000 (0.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) HEK-293T transfected with a human Tau 1N3R expression vector containing a his tag and (B) HEK-293T transfected with a human Tau 1N4R expression vector containing a his tag. No staining on (C) HEK-293T transfected with a human Tau 0N3R expression vector containing a his tag, (D) HEK-293T transfected with a human Tau 2N3R expression vector containing a his tag, (E) HEK-293T transfected with a human Tau 0N4R expression vector containing a his tag, (F) HEK-293T transfected with a human Tau 2N4R expression vector containing a his tag, and (G) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (ab314889), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] (ab314889)

    Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labeling 1N Tau with ab314889 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human hippocampus.The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889), expandable thumbnail

    Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-Tau antibody [EPR22524-95] (Anti-Tau antibody [EPR22524-95] ab254256) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889) at 1/1000 dilution

    Lane 1: His-tagged Tau 0N3R human recombinant protein at 50 ng

    Lane 2: His-tagged Tau 0N4R human recombinant protein at 50 ng

    Lane 3: His-tagged Tau 1N3R human recombinant protein at 10 ng

    Lane 4: His-tagged Tau 1N4R human recombinant protein at 10 ng

    Lane 5: His-tagged Tau 2N3R human recombinant protein at 50 ng

    Lane 6: His-tagged Tau 2N4R human recombinant protein at 50 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 75 kDa

    Exposure time: 180s

  • Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889), expandable thumbnail

    Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: spleen (PMID: 24386422).

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

    All lanes: Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889) at 1/1000 dilution

    Lane 1: Rat hippocampus tissue lysate at 80 µg

    Lane 2: Rat spleen tissue lysate at 80 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Observed band size: 55 kDa, 15 kDa

    Exposure time: 59s

  • Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889), expandable thumbnail

    Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: spleen (PMID: 24386422).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889) at 1/1000 dilution

    Lane 1: Human hippocampus tissue lysate at 80 µg

    Lane 2: Human spleen tissue lysate at 80 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Developed using the ECL technique.

    Observed band size: 55 kDa, 36 kDa

    Exposure time: 15s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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