Rabbit Recombinant Monoclonal TAU antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Transfected cell line - Human, Rat, Transfected cell lysate - Human, Recombinant fragment - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | WB | ICC/IF | IP | |
---|---|---|---|---|
Human | Tested | Tested | Expected | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Tested | Not recommended | Not recommended |
Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended |
Transfected cell line - Human | Tested | Not recommended | Tested | Not recommended |
Transfected cell lysate - Human | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line - Human | Dilution info 1/20000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Transfected cell lysate - Human, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Transfected cell line - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Transfected cell lysate - Human, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat, Mouse, Transfected cell line - Human, Transfected cell lysate - Human, Recombinant fragment - Human | Dilution info - | Notes - |
Select an associated product type
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
MAPT
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Rabbit Recombinant Monoclonal TAU antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Transfected cell line - Human, Rat, Transfected cell lysate - Human, Recombinant fragment - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR25206-82
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
1N Tau also known as human Tau protein plays a role in stabilizing microtubules in neurons. It is a low molecular weight protein with a mass of around 45 kDa. The expression of 1N Tau occurs mostly in the central nervous system but is also present in smaller quantities in peripheral nervous tissues. This protein binds directly to microtubules through its repeat domains which forms a tight and stable structure necessary for proper neuronal function.
1N Tau is critical in the assembly and stabilization of the neuronal cytoskeleton. It normally functions as part of a larger microtubule-associated protein complex. By promoting microtubule polymerization it helps maintain the axonal transport system necessary for neuronal communication. The functional activity of 1N Tau can be controlled by phosphorylation a process that can modulate its binding affinity to microtubules.
The cellular processes involving 1N Tau often intersect with important signaling cascades such as the MAPK/ERK and PI3K/Akt pathways. The alterations in phosphorylation states regulate its interaction with other microtubule-associated proteins like MAP2 and MAP4. This regulation ensures the stability of the neuronal cytoskeleton and influences processes like cell growth and survival impacting overall neuronal health and function.
Abnormal 1N Tau phosphorylation and aggregation are closely associated with neurodegenerative diseases such as Alzheimer's disease and frontotemporal dementia. These conditions often show the presence of paired helical filaments formed by hyperphosphorylated 1N Tau. Interaction with amyloid precursor protein (APP) in Alzheimer's disease suggests a link between Tau aggregation and amyloid beta pathology which contributes to neuronal dysfunction and cognitive decline.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling 1N Tau with ab314889 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum.The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling 1N Tau with ab314889 at 1/50 (10.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a human Tau 1N3R and Tau 1N4R expression vector containing a myc-tag, showing no staining in Tau 0N4R/0N3R/2N3R/2N4R(WT) and empty expression vector containing a myc-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling 1N Tau with ab314889 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human spleen. The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution, Anti-Tau antibody [EPR22524-95] (Anti-Tau antibody [EPR22524-95] ab254256) staining at 1/1000 dilution.
All lanes: Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human Tau 0N3R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human Tau 0N4R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human Tau 1N3R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 5: 293T cells transfected with a human Tau 1N4R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 6: 293T cells transfected with a human Tau 2N3R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 7: 293T cells transfected with a human Tau 2N4R expression vector containing a His-tag, whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 37-75 kDa, 15 kDa
Exposure time: 6s
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human Tau 1N3R expression vector containing a his tag. (B) HEK-293T transfected with a human Tau 1N4R expression vector containing a his tag. (C) HEK-293T transfected with a human Tau 0N3R expression vector containing a his tag. (D) HEK-293T transfected with a human Tau 2N3R expression vector containing a his tag. (E) HEK-293T transfected with a human Tau 0N4R expression vector containing a his tag. (F) HEK-293T transfected with a human Tau 2N4R expression vector containing a his tag. (G) HEK-293T cells transfected with empty vector containing a his tag. tissue labeling 1N Tau with ab314889 at 1/20000 (0.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a human Tau 1N3R expression vector containing a his tag and (B) HEK-293T transfected with a human Tau 1N4R expression vector containing a his tag. No staining on (C) HEK-293T transfected with a human Tau 0N3R expression vector containing a his tag, (D) HEK-293T transfected with a human Tau 2N3R expression vector containing a his tag, (E) HEK-293T transfected with a human Tau 0N4R expression vector containing a his tag, (F) HEK-293T transfected with a human Tau 2N4R expression vector containing a his tag, and (G) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labeling 1N Tau with ab314889 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human hippocampus.The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-Tau antibody [EPR22524-95] (Anti-Tau antibody [EPR22524-95] ab254256) staining at 1/1000 dilution.
All lanes: Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889) at 1/1000 dilution
Lane 1: His-tagged Tau 0N3R human recombinant protein at 50 ng
Lane 2: His-tagged Tau 0N4R human recombinant protein at 50 ng
Lane 3: His-tagged Tau 1N3R human recombinant protein at 10 ng
Lane 4: His-tagged Tau 1N4R human recombinant protein at 10 ng
Lane 5: His-tagged Tau 2N3R human recombinant protein at 50 ng
Lane 6: His-tagged Tau 2N4R human recombinant protein at 50 ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: spleen (PMID: 24386422).
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
All lanes: Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889) at 1/1000 dilution
Lane 1: Rat hippocampus tissue lysate at 80 µg
Lane 2: Rat spleen tissue lysate at 80 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Observed band size: 55 kDa, 15 kDa
Exposure time: 59s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: spleen (PMID: 24386422).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-1N Tau antibody [EPR25206-82] (ab314889) at 1/1000 dilution
Lane 1: Human hippocampus tissue lysate at 80 µg
Lane 2: Human spleen tissue lysate at 80 µg
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Developed using the ECL technique.
Observed band size: 55 kDa, 36 kDa
Exposure time: 15s
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