Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free
- BOND RX™ Validated
- RabMAb
- Recombinant
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Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Transfected cell line - Human, Rat, Transfected cell lysate - Human, Recombinant fragment - Human samples.
View Alternative Names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)](https://content.abcam.com/products/images/1n-tau-antibody-epr25206-82-bsa-and-azide-free-ab314890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423628.jpg/_jcr_content/renditions/webp-768.webp)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)
This data was developed using ab314889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labeling 1N Tau with ab314889 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human hippocampus.The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)](https://content.abcam.com/products/images/1n-tau-antibody-epr25206-82-bsa-and-azide-free-ab314890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423629.jpg/_jcr_content/renditions/webp-768.webp)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)
This data was developed using ab314889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human Tau 1N3R expression vector containing a his tag. (B) HEK-293T transfected with a human Tau 1N4R expression vector containing a his tag. (C) HEK-293T transfected with a human Tau 0N3R expression vector containing a his tag. (D) HEK-293T transfected with a human Tau 2N3R expression vector containing a his tag. (E) HEK-293T transfected with a human Tau 0N4R expression vector containing a his tag. (F) HEK-293T transfected with a human Tau 2N4R expression vector containing a his tag. (G) HEK-293T cells transfected with empty vector containing a his tag. tissue labeling 1N Tau with ab314889 at 1/20000 (0.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a human Tau 1N3R expression vector containing a his tag and (B) HEK-293T transfected with a human Tau 1N4R expression vector containing a his tag. No staining on (C) HEK-293T transfected with a human Tau 0N3R expression vector containing a his tag, (D) HEK-293T transfected with a human Tau 2N3R expression vector containing a his tag, (E) HEK-293T transfected with a human Tau 0N4R expression vector containing a his tag, (F) HEK-293T transfected with a human Tau 2N4R expression vector containing a his tag, and (G) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)](https://content.abcam.com/products/images/1n-tau-antibody-epr25206-82-bsa-and-azide-free-ab314890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423630.jpg/_jcr_content/renditions/webp-768.webp)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)
This data was developed using ab314889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling 1N Tau with ab314889 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control : no staining on human spleen. The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)](https://content.abcam.com/products/images/1n-tau-antibody-epr25206-82-bsa-and-azide-free-ab314890--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423627.jpg/_jcr_content/renditions/webp-768.webp)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)
This data was developed using ab314889, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling 1N Tau with ab314889 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum.The section was incubated with ab314889 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunocytochemistry/ Immunofluorescence - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)](https://content.abcam.com/products/images/1n-tau-antibody-epr25206-82-bsa-and-azide-free-ab314890--immunocytochemistry-immunofluorescence-img423662.jpg/_jcr_content/renditions/webp-768.webp)
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)
This data was developed using ab314889, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling 1N Tau with ab314889 at 1/50 (10.2 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a human Tau 1N3R and Tau 1N4R expression vector containing a myc-tag, showing no staining in Tau 0N4R/0N3R/2N3R/2N4R(WT) and empty expression vector containing a myc-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Western blot - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)](https://content.abcam.com/products/images/1n-tau-antibody-epr25206-82-bsa-and-azide-free-ab314890--western-blot-img423598.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Supplier Data
Western blot - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)
This data was developed using ab314889, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Negative control : spleen (PMID : 24386422).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-1N Tau antibody [EPR25206-82] (<a href='/en-us/products/primary-antibodies/1n-tau-antibody-epr25206-82-ab314889'>ab314889</a>) at 1/1000 dilution
Lane 1:
Human hippocampus tissue lysate at 80 µg
Lane 2:
Human spleen tissue lysate at 80 µg
Secondary
All lanes:
Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution
Observed band size: 55 kDa,36 kDa
true
Exposure time: 15s
![Western blot - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)](https://content.abcam.com/products/images/1n-tau-antibody-epr25206-82-bsa-and-azide-free-ab314890--western-blot-img423599.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Supplier Data
Western blot - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)
This data was developed using ab314889, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Negative control : spleen (PMID : 24386422).
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.
All lanes:
Western blot - Anti-1N Tau antibody [EPR25206-82] (<a href='/en-us/products/primary-antibodies/1n-tau-antibody-epr25206-82-ab314889'>ab314889</a>) at 1/1000 dilution
Lane 1:
Rat hippocampus tissue lysate at 80 µg
Lane 2:
Rat spleen tissue lysate at 80 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Observed band size: 55 kDa,15 kDa
false
Exposure time: 59s
![Western blot - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)](https://content.abcam.com/products/images/1n-tau-antibody-epr25206-82-bsa-and-azide-free-ab314890--western-blot-img423600.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Supplier Data
Western blot - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)
This data was developed using ab314889, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.
In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) staining at 1/5000 dilution, Anti-Tau antibody [EPR22524-95] (ab254256) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-1N Tau antibody [EPR25206-82] (<a href='/en-us/products/primary-antibodies/1n-tau-antibody-epr25206-82-ab314889'>ab314889</a>) at 1/1000 dilution
Lane 1:
293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg
Lane 2:
293T cells transfected with a human Tau 0N3R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 3:
293T cells transfected with a human Tau 0N4R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 4:
293T cells transfected with a human Tau 1N3R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 5:
293T cells transfected with a human Tau 1N4R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 6:
293T cells transfected with a human Tau 2N3R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 7:
293T cells transfected with a human Tau 2N4R expression vector containing a His-tag, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 37-75 kDa,15 kDa
false
Exposure time: 6s
![Western blot - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)](https://content.abcam.com/products/images/1n-tau-antibody-epr25206-82-bsa-and-azide-free-ab314890--western-blot-img423601.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Supplier Data
Western blot - Anti-1N Tau antibody [EPR25206-82] - BSA and Azide free (AB314890)
This data was developed using ab314889, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, Anti-Tau antibody [EPR22524-95] (ab254256) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-1N Tau antibody [EPR25206-82] (<a href='/en-us/products/primary-antibodies/1n-tau-antibody-epr25206-82-ab314889'>ab314889</a>) at 1/1000 dilution
Lane 1:
His-tagged Tau 0N3R human recombinant protein at 50 ng
Lane 2:
His-tagged Tau 0N4R human recombinant protein at 50 ng
Lane 3:
His-tagged Tau 1N3R human recombinant protein at 10 ng
Lane 4:
His-tagged Tau 1N4R human recombinant protein at 10 ng
Lane 5:
His-tagged Tau 2N3R human recombinant protein at 50 ng
Lane 6:
His-tagged Tau 2N4R human recombinant protein at 50 ng
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 75 kDa
false
Exposure time: 180s
Reactivity data
Product details
ab314890 is the carrier-free version of ab314889.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
1N Tau is critical in the assembly and stabilization of the neuronal cytoskeleton. It normally functions as part of a larger microtubule-associated protein complex. By promoting microtubule polymerization it helps maintain the axonal transport system necessary for neuronal communication. The functional activity of 1N Tau can be controlled by phosphorylation a process that can modulate its binding affinity to microtubules.
Pathways
The cellular processes involving 1N Tau often intersect with important signaling cascades such as the MAPK/ERK and PI3K/Akt pathways. The alterations in phosphorylation states regulate its interaction with other microtubule-associated proteins like MAP2 and MAP4. This regulation ensures the stability of the neuronal cytoskeleton and influences processes like cell growth and survival impacting overall neuronal health and function.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com