AB251232

Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal 2 Hydroxy phytanoyl CoA lyase/HACL1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

HPCL, HPCL2, PHYH2, HSPC279, HACL1, 2-hydroxyacyl-CoA lyase 1, 2-hydroxyphytanoyl-CoA lyase, Phytanoyl-CoA 2-hydroxylase 2, 2-HPCL

3 Images

Supplier Data

Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (AB251232)

This data was developed using ab197025, the same antibody clone in a different buffer formulation.

2 Hydroxy phytanoyl CoA lyase/HACL1 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab197025 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197025 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : HeLa whole cell extract (Input) 10 μg. Lane 2 : ab197025 IP in HeLa whole cell extract. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab197025 in HeLa whole cell extract.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (<a href='/en-us/products/primary-antibodies/2-hydroxy-phytanoyl-coa-lyase-hacl1-antibody-epr16812-ab197025'>ab197025</a>)

Predicted band size: 64 kDa

false

Supplier Data

Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (AB251232)

This data was developed using ab197025, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (<a href='/en-us/products/primary-antibodies/2-hydroxy-phytanoyl-coa-lyase-hacl1-antibody-epr16812-ab197025'>ab197025</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Rat brain tissue lysate at 10 µg

Lane 4:

Rat heart tissue lysate at 10 µg

Lane 5:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 6:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 7:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 8:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Supplier Data

Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (AB251232)

This data was developed using ab197025, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16812

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251232 is the carrier-free version of ab197025.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

2-Hydroxyphytanoyl-CoA lyase (HACL1) also known as phytanoyl-CoA hydroxylase is an enzyme that plays a mechanical role in breaking down branched-chain fatty acids specifically phytanic acid. HACL1 catalyzes the cleavage of 2-hydroxyphytanoyl-CoA to pristanal producing formyl-CoA in the process. This enzyme has a molecular mass of approximately 50 kDa. HACL1 is mainly expressed in peroxisomes which are subcellular compartments involved in lipid metabolism.

Biological function summary

2-Hydroxyphytanoyl-CoA lyase serves a critical function in the metabolism of fatty acids derived from dietary sources especially chlorophyll-rich plant materials. It operates as part of the α-oxidation pathway a unique metabolic route for the degradation of phytanic acid which is a type of branched-chain fatty acid that cannot be processed by β-oxidation. HACL1 is not known to be part of a larger enzyme complex unlike some other enzymes involved in fatty acid metabolism.

Pathways

The α-oxidation pathway is where HACL1 plays its significant role. This pathway starts with the conversion of phytanic acid into 2-hydroxyphytanoyl-CoA which HACL1 then cleaves. The α-oxidation pathway connects to the broader lipid metabolism processes in the body. Related proteins involved in these pathways include fatty aldehyde dehydrogenase (FALDH) which further processes the metabolic products of HACL1 activity.

Disruptions in the function of HACL1 can lead to Refsum disease which is characterized by the accumulation of phytanic acid in the body resulting in neurological symptoms. HACL1's role can be connected to proteins involved in peroxisomal functions like peroxisome biogenesis factor proteins whose dysfunction might also contribute to metabolic disorders similar to Refsum disease such as Zellweger spectrum disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Peroxisomal 2-OH acyl-CoA lyase involved in the cleavage (C1 removal) reaction in the fatty acid alpha-oxydation in a thiamine pyrophosphate (TPP)-dependent manner (PubMed : 10468558, PubMed : 21708296, PubMed : 28289220). Involved in the degradation of 3-methyl-branched fatty acids like phytanic acid and the shortening of 2-hydroxy long-chain fatty acids (PubMed : 10468558, PubMed : 21708296, PubMed : 28289220). Plays a significant role in the biosynthesis of heptadecanal in the liver (By similarity).

See full target information HACL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:3361-3370 PubMed33682317

2021

TNFAIP8 regulates gastric cancer growth via mTOR-Akt-ULK1 pathway and autophagy signals.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Chen,Jianguo Zhang,Chenyang Dong,Dongsheng Li,Yuehan Yin,Wenhai Yu,Yuezhi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free

Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (AB251232)

Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (AB251232)

Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (AB251232)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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