AB251530

Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal 2-methyladenosine (m2A) antibody. Carrier free. Suitable for IP, ELISA and reacts with Modified Nucleic Acid samples.

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ELISA - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (AB251530)

ELISA

Supplier Data

ELISA - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (AB251530)

This data was developed using ab211495, the same antibody clone in a different buffer formulation.

Biotinylated m2A (modified) and A (unmodified) oligonucleotides with the below sequence were coated onto wells of a 96 well plate.

Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[ m2A]*.mN.mN.mN.mN.mN 3'

Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

ELISA was performed on 1.0 μg/ml of antigen using ab211495 at a concentration range of 0.005-4.000 μg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

Supplier Data

Immunoprecipitation - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (AB251530)

This data was developed using ab211495, the same antibody clone in a different buffer formulation.

The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

ab211495 (0.2 μg) was coated into Dynabeads^® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

Lane 1 : Buffer only.

Lane 2 : Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[ m2A]*.mN.mN.mN.mN.mN 3'

Lane 3 : Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

Blocking buffer : 5% NFDM/TBST

Dilution buffer : TBST/0.1% Triton X-100/1 mM EDTA.

All lanes:

Immunoprecipitation - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] (<a href='/en-us/products/primary-antibodies/2-methyladenosine-m2a-antibody-epr-19851-59-ab211495'>ab211495</a>)

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Unconjugated

Anti-2-methyladenosine (m2A) antibody [EPR -19851-59]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR -19851-59

Isotype

IgG

Carrier free

Yes

Applications

ELISA, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Has been developed to discriminate between the modified base 2-methyladenosine (m2A) and the unmodified counterpart Adenosine (A).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }

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Product details

ab251530 is the carrier-free version of ab211495.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

2-methyladenosine (m2A) is a modified nucleoside that plays a significant role in RNA structures impacting RNA stability and function. It is an adenosine derivative where a methyl group is added to the nitrogen at position 2 of the adenine ring altering its chemical properties. This modification has a relatively small molecular mass of approximately 281.3 Da. m2A is found primarily in tRNA and rRNA molecules within the cell contributing to their proper folding and stability. It is ubiquitously expressed across various tissues reflecting its broad functional significance.

Biological function summary

The modification of RNA by 2-methyladenosine contributes significantly to RNA processing and function. It plays a role in the structural integrity and proper folding of tRNA and rRNA by maintaining the stability of the RNA molecules they modify. m2A acts within ribonucleoprotein complexes where it influences the recognition and interaction between RNA and protein components. This modification ensures the accuracy of translation by maintaining the correct conformation of the RNA molecules involved in protein synthesis.

Pathways

2-methyladenosine engages in essential biological processes such as translation and RNA processing. It is involved in the ribosome biogenesis pathway which is important for protein synthesis in cells. Within this pathway m2A interacts with other RNA modifications and proteins like the ribosomal proteins and various RNA-binding proteins that guide RNA through the maturation process. The presence of m2A aids the efficient assembly of ribosomal units necessary for the synthesis of proteins.

Altered levels of 2-methyladenosine have been implicated in disorders related to dysfunction in protein synthesis. Aberrant m2A modification has been linked to certain cancers wherein improper RNA processing might contribute to uncontrolled cellular proliferation. Additionally it relates to neurodegenerative diseases where misfolded proteins result from translation dysregulation. In this context m2A modifications can affect the function of proteins such as ribosome-associated proteins and those involved in RNA methylation influencing disease outcomes through changes in RNA stability and processing.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 2-methyladenosine (m2A)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free

ELISA - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (AB251530)

Immunoprecipitation - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] - BSA and Azide free (AB251530)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise