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AB219464

Anti-25-OH Vitamin D3 antibody [RM3]

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(3 Publications)

Rabbit Recombinant Monoclonal 25-OH Vitamin D3 antibody. Suitable for cELISA and reacts with Chemical samples. Cited in 3 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to 25-OH Vitamin D3.
2 Images
Competitive ELISA - Anti-25-OH Vitamin D3 antibody [RM3] (AB219464)
  • cELISA

Supplier Data

Competitive ELISA - Anti-25-OH Vitamin D3 antibody [RM3] (AB219464)

Cross activity data of ab219464 at 100 ng/ml.

Competitive ELISA - Anti-25-OH Vitamin D3 antibody [RM3] (AB219464)
  • cELISA

Supplier Data

Competitive ELISA - Anti-25-OH Vitamin D3 antibody [RM3] (AB219464)

Competitive ELISA data using ab219464

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM3

Isotype

IgG

Carrier free

No

Applications

cELISA

applications

Specificity

ab219464 reacts to 25-OH Vitamin D3. It does not cross-react with Vitamin D3, Vitamin D2, and 25-OH Vitamin D2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p>Use 0.5 ng/well - 1.5 ng/well on a 96well plate pre-coated with a goat anti-rabbit IgG polyclonal antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab219464 was purified from an animal origin–free culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

25-OH Vitamin D3 also known as calcidiol or 25-hydroxycholecalciferol serves as a prohormone and the main storage form of vitamin D in the human body. It has a molecular mass of approximately 400.64 g/mol. The liver expresses it where the conversion from vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol) occurs. It circulates in the bloodstream and acts as a reservoir for the active form of vitamin D.
Biological function summary

25-hydroxyvitamin D3 is important to maintaining calcium and phosphate homeostasis. It is not part of a protein complex in its function as a circulatory prohormone. The kidneys convert it into the active form 125-dihydroxyvitamin D3 (calcitriol) which then interacts with the vitamin D receptor (VDR) to modulate gene expression related to calcium metabolism.

Pathways

This vitamin D3 metabolite holds importance in the calcium signaling and bone remodeling pathways. Its conversion to calcitriol aids in the regulation of parathyroid hormone (PTH) ensuring calcium balance in the bloodstream. The pathway includes elements such as the VDR and CYP27B1 which are necessary for its activation and action.

Alterations in 25-OH Vitamin D3 levels influence conditions like osteoporosis and rickets. Both conditions relate to disruptions in calcium homeostasis. Proteins like the VDR and CYP27B1 are involved in these disorders influencing bone density and mineralization through the modulation of calcidiol and calcitriol levels.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information 25-OH Vitamin D3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2306038 PubMed38381100

2024

Label-Free Metal-Oxide Transistor Biosensors for Metabolite Detection in Human Saliva.

Applications

Unspecified application

Species

Unspecified reactive species

Abhinav Sharma,Hendrik Faber,Wejdan S AlGhamdi,Dipti Naphade,Yen-Hung Lin,Martin Heeney,Thomas D Anthopoulos

Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology 35:49-55 PubMed31532316

2019

Expression of vitamin D and vitamin D receptor in chorionic villous in missed abortion.

Applications

Unspecified application

Species

Unspecified reactive species

Olesya Bespalova,Margarita Bakleicheva,Irina Kovaleva,Gulrukhsor Tolibova,Tatiana Tral,Igor Kogan

Experimental and therapeutic medicine 16:1701-1706 PubMed30186390

2018

Expression of miR-376 in blood of pregnant women with preeclampsia and its effect on 25-hydroxyvitamin D.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlan Li,Jiangdong Du,Zhan Wang,Chenghong Wang,Jianhua Bai,Su Zhang
View all publications
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