Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-Fr | IHC-P | WB | |
---|---|---|---|
Human | Not recommended | Not recommended | Tested |
Mouse | Not recommended | Not recommended | Tested |
Rat | Not recommended | Not recommended | Tested |
Recombinant fragment - Human | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
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Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
TAU, MAPT, MSTD, PPND, TAU, pTau, DDPAC, MAPTL, MSTD, MTBT1, MTBT2, Mtapt, PPND, Tau-C, DDPAC, FTDP17, MAPTL, MTBT1, MTBT2, RNPTAU, FTDP 17, PHF tau, PHF-tau, 2N3R Tau, 2N4R Tau, AI413597, AW045860, FLJ31424, PPP1R103, MGC134287, MGC138549, MGC156663, TAU_HUMAN, Paired helical filament tau, Paired helical filament-tau, Paired helical filament tau, Neurofibrillary tangle protein, Tauopathy and respiratory failure, Microtubule associated protein tau, Microtubule-associated protein tau, Tauopathy and respiratory failure, included, Microtubule associated protein tau isoform 4, Microtubule associated protein tau isoform 4, Protein phosphatase 1, regulatory subunit 103, G protein beta1/gamma2 subunit interacting factor 1
Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples.
TAU, MAPT, MSTD, PPND, TAU, pTau, DDPAC, MAPTL, MSTD, MTBT1, MTBT2, Mtapt, PPND, Tau-C, DDPAC, FTDP17, MAPTL, MTBT1, MTBT2, RNPTAU, FTDP 17, PHF tau, PHF-tau, 2N3R Tau, 2N4R Tau, AI413597, AW045860, FLJ31424, PPP1R103, MGC134287, MGC138549, MGC156663, TAU_HUMAN, Paired helical filament tau, Paired helical filament-tau, Paired helical filament tau, Neurofibrillary tangle protein, Tauopathy and respiratory failure, Microtubule associated protein tau, Microtubule-associated protein tau, Tauopathy and respiratory failure, included, Microtubule associated protein tau isoform 4, Microtubule associated protein tau isoform 4, Protein phosphatase 1, regulatory subunit 103, G protein beta1/gamma2 subunit interacting factor 1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR21723
Affinity purification Protein A
The specificity of this antibody refers to P10636-8.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
The 2N Tau protein also known as tau or tau protein is a microtubule-associated protein with a mass of approximately 78 kDa. It stabilizes microtubules and is critical for maintaining the axonal transport in neurons. It is highly expressed in the central nervous system particularly in the neurons and becomes hyperphosphorylated under certain conditions. Tau belongs to the family of microtubule-associated proteins and has six isoforms generated through alternative splicing.
The microtubule-associated protein tau plays a significant role in neuron stability and function by promoting the assembly and stabilization of microtubules. It achieves this through its binding affinity for tubulin which is necessary for the proper functioning of the neuronal cytoskeleton. Tau does not function as part of a complex but its interaction with microtubules is critical for cellular operations including intracellular transport and signaling.
Tau functions in processes essential for cellular transport and microtubule dynamics regulation. It is involved in the MAPK/ERK pathway impacting cellular signaling and stress responses. Tau's regulatory role in microtubule stability connects it with proteins such as tubulin and other microtubule-associated proteins. This interaction ensures proper transmission of signals within neurons and affects cell cycle regulation.
Tau dysregulation and hyperphosphorylation relate closely to Alzheimer's disease leading to neurofibrillary tangles that disrupt neuron function. In Alzheimer's tau's interaction with proteins like beta-amyloid is critical for disease progression. Additionally tau is implicated in frontotemporal dementia where its misfolding and aggregation significantly impact neuronal survival and function disrupting communication with proteins important for brain function.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
Exposure time : Lanes 1-3: 3 minutes; Lanes 4-6: 59 seconds; Lane 7: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Negative control: Mouse stomach, human stomach. PMID:8752131; PMID:11727254.
All lanes: Western blot - Anti-2N Tau antibody [EPR21723] (ab218316) at 1/1000 dilution
Lane 1: Human hippocampus lysate at 20 µg
Lane 2: Human brain lysate at 20 µg
Lane 3: Human stomach lysate at 20 µg
Lane 4: Mouse hippocampus lysate at 20 µg
Lane 5: Mouse brain lysate at 20 µg
Lane 6: Mouse stomach lysate at 20 µg
Lane 7: Rat hippocampus lysate at 20 µg
Lanes 1 - 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lanes 4 - 7: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 78 kDa
Observed band size: 36-75 kDa
Exposure time : Lanes 1-3: 3 minutes; Lanes 4-6: 59 seconds; Lane 7: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Negative control: Mouse stomach, human stomach. PMID:8752131; PMID:11727254.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218316).
Exposure time : Lane 1: 1 second; Lanes 2-8: 3 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
This antibody specifically recognizes 2N3R and 2N4R tau recombinant proteins. The lower bands maybe degraded tau fragments (PMID:28045602).
All lanes: Western blot - Anti-2N Tau antibody [EPR21723] (ab218316) at 1/1000 dilution
Lane 1: His-tagged human 2N3R Tau recombinant protein (aa1-410), 10 ng
Lane 2: His-tagged human 2N4R Tau recombinant protein (aa1-441), 10 ng
Lane 3: His-tagged human 0N3R Tau recombinant protein (aa1-352), 10 ng
Lane 4: His-tagged human 0N4R Tau recombinant protein (aa1-383), 10 ng
Lane 5: His-tagged human 1N3R Tau recombinant protein (aa1-381), 10 ng
Lane 6: His-tagged human 1N4R Tau recombinant protein (aa1-412), 10 ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 78 kDa
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