Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples.
Images
![Western blot - Anti-2N Tau antibody [EPR21723] (AB218316), expandable thumbnail](https://content.abcam.com/products/images/2n-tau-antibody-epr21723-ab218316--western-blot-img27857.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-2N Tau antibody [EPR21723] (AB218316)")
![Western blot - Anti-2N Tau antibody [EPR21723] (AB218316), expandable thumbnail](https://content.abcam.com/products/images/2n-tau-antibody-epr21723-ab218316--western-blot-img54266.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-2N Tau antibody [EPR21723] (AB218316)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | IHC-P | WB | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Tested |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
1 products for Alternative Product
Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
TAU, MAPT, MSTD, PPND, TAU, pTau, DDPAC, MAPTL, MSTD, MTBT1, MTBT2, Mtapt, PPND, Tau-C, DDPAC, FTDP17, MAPTL, MTBT1, MTBT2, RNPTAU, FTDP 17, PHF tau, PHF-tau, 2N3R Tau, 2N4R Tau, AI413597, AW045860, FLJ31424, PPP1R103, MGC134287, MGC138549, MGC156663, TAU_HUMAN, Paired helical filament tau, Paired helical filament-tau, Paired helical filament tau, Neurofibrillary tangle protein, Tauopathy and respiratory failure, Microtubule associated protein tau, Microtubule-associated protein tau, Tauopathy and respiratory failure, included, Microtubule associated protein tau isoform 4, Microtubule associated protein tau isoform 4, Protein phosphatase 1, regulatory subunit 103, G protein beta1/gamma2 subunit interacting factor 1
Recommended products
Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples.
Alternative names
TAU, MAPT, MSTD, PPND, TAU, pTau, DDPAC, MAPTL, MSTD, MTBT1, MTBT2, Mtapt, PPND, Tau-C, DDPAC, FTDP17, MAPTL, MTBT1, MTBT2, RNPTAU, FTDP 17, PHF tau, PHF-tau, 2N3R Tau, 2N4R Tau, AI413597, AW045860, FLJ31424, PPP1R103, MGC134287, MGC138549, MGC156663, TAU_HUMAN, Paired helical filament tau, Paired helical filament-tau, Paired helical filament tau, Neurofibrillary tangle protein, Tauopathy and respiratory failure, Microtubule associated protein tau, Microtubule-associated protein tau, Tauopathy and respiratory failure, included, Microtubule associated protein tau isoform 4, Microtubule associated protein tau isoform 4, Protein phosphatase 1, regulatory subunit 103, G protein beta1/gamma2 subunit interacting factor 1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR21723
- Purification technique
Affinity purification Protein A
- Specificity
The specificity of this antibody refers to P10636-8.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- Activity summary
The 2N Tau protein also known as tau or tau protein is a microtubule-associated protein with a mass of approximately 78 kDa. It stabilizes microtubules and is critical for maintaining the axonal transport in neurons. It is highly expressed in the central nervous system particularly in the neurons and becomes hyperphosphorylated under certain conditions. Tau belongs to the family of microtubule-associated proteins and has six isoforms generated through alternative splicing.
- Biological function summary
The microtubule-associated protein tau plays a significant role in neuron stability and function by promoting the assembly and stabilization of microtubules. It achieves this through its binding affinity for tubulin which is necessary for the proper functioning of the neuronal cytoskeleton. Tau does not function as part of a complex but its interaction with microtubules is critical for cellular operations including intracellular transport and signaling.
- Pathways
Tau functions in processes essential for cellular transport and microtubule dynamics regulation. It is involved in the MAPK/ERK pathway impacting cellular signaling and stress responses. Tau's regulatory role in microtubule stability connects it with proteins such as tubulin and other microtubule-associated proteins. This interaction ensures proper transmission of signals within neurons and affects cell cycle regulation.
- Associated diseases and disorders
Tau dysregulation and hyperphosphorylation relate closely to Alzheimer's disease leading to neurofibrillary tangles that disrupt neuron function. In Alzheimer's tau's interaction with proteins like beta-amyloid is critical for disease progression. Additionally tau is implicated in frontotemporal dementia where its misfolding and aggregation significantly impact neuronal survival and function disrupting communication with proteins important for brain function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-2N Tau antibody [EPR21723] (ab218316), expandable thumbnail](https://content.abcam.com/products/images/2n-tau-antibody-epr21723-ab218316--western-blot-img27857.jpg "Western blot - Anti-2N Tau antibody [EPR21723] (ab218316)")
Western blot - Anti-2N Tau antibody [EPR21723] (ab218316)
Exposure time : Lanes 1-3: 3 minutes; Lanes 4-6: 59 seconds; Lane 7: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Negative control: Mouse stomach, human stomach. PMID:8752131; PMID:11727254.
All lanes: Western blot - Anti-2N Tau antibody [EPR21723] (ab218316) at 1/1000 dilution
Lane 1: Human hippocampus lysate at 20 µg
Lane 2: Human brain lysate at 20 µg
Lane 3: Human stomach lysate at 20 µg
Lane 4: Mouse hippocampus lysate at 20 µg
Lane 5: Mouse brain lysate at 20 µg
Lane 6: Mouse stomach lysate at 20 µg
Lane 7: Rat hippocampus lysate at 20 µg
SecondaryLanes 1 - 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lanes 4 - 7: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 78 kDa
Observed band size: 36-75 kDa
Exposure time : Lanes 1-3: 3 minutes; Lanes 4-6: 59 seconds; Lane 7: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Negative control: Mouse stomach, human stomach. PMID:8752131; PMID:11727254.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218316).
![Western blot - Anti-2N Tau antibody [EPR21723] (ab218316), expandable thumbnail](https://content.abcam.com/products/images/2n-tau-antibody-epr21723-ab218316--western-blot-img54266.jpg "Western blot - Anti-2N Tau antibody [EPR21723] (ab218316)")
Western blot - Anti-2N Tau antibody [EPR21723] (ab218316)
Exposure time : Lane 1: 1 second; Lanes 2-8: 3 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
This antibody specifically recognizes 2N3R and 2N4R tau recombinant proteins. The lower bands maybe degraded tau fragments (PMID:28045602).
All lanes: Western blot - Anti-2N Tau antibody [EPR21723] (ab218316) at 1/1000 dilution
Lane 1: His-tagged human 2N3R Tau recombinant protein (aa1-410), 10 ng
Lane 2: His-tagged human 2N4R Tau recombinant protein (aa1-441), 10 ng
Lane 3: His-tagged human 0N3R Tau recombinant protein (aa1-352), 10 ng
Lane 4: His-tagged human 0N4R Tau recombinant protein (aa1-383), 10 ng
Lane 5: His-tagged human 1N3R Tau recombinant protein (aa1-381), 10 ng
Lane 6: His-tagged human 1N4R Tau recombinant protein (aa1-412), 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 78 kDa
Downloads
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