Skip to main content

Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282) is a mouse monoclonal antibody detecting 3-Nitrotyrosine in Western Blot, Flow Cytometry, IP, ICC/IF, ELISA.



- Over 30 publications


Images

Flow Cytometry - Anti-3-Nitrotyrosine antibody [7A12AF6] (AB110282), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-3-Nitrotyrosine antibody [7A12AF6] (AB110282), expandable thumbnail
  • Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (AB110282), expandable thumbnail
  • Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (AB110282), expandable thumbnail
  • Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (AB110282), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow CytICC/IFIn-Cell ELISA
Chemical
Tested
Expected
Tested
Tested
Expected

Tested
Tested

Species
Chemical
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Chemical
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Chemical
Dilution info
1 µg/mL
Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Chemical
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Chemical
Dilution info
4 µg/mL
Notes

(0.4 μg/well).

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Alternative names

3-NT, 3NT

Recommended products

Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282) is a mouse monoclonal antibody detecting 3-Nitrotyrosine in Western Blot, Flow Cytometry, IP, ICC/IF, ELISA.



- Over 30 publications

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
7A12AF6
Purification technique
Proprietary technique
Specificity

ab110282 was developed to recognize only protein-bound nitrotyrosine and so is a sensitive tool for measuring protein-specific modifications from oxidative stress.

Light chain type
kappa
Concentration
Loading...
Purification notes

The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

What is this antibody validated in?


Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA.

Trusted by the scientific community


Anti-3-Nitrotyrosine [7A12AF6] (ab110282) was first used in a scientific publication in 2011 and has been cited over 30 times in peer-reviewed journals.

Other related products


We have a range of other formats of antibody clone [7A12AF6] also available for your convenience:
ab110282, HRP - HRP Anti-3-Nitrotyrosine antibody [7A12AF6] ab198491



Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

3-Nitrotyrosine forms when peroxynitrite interacts with tyrosine residues in proteins. It is a marker of nitrosative stress and oxidative damage with the molecular mass of the modified residue remaining similar to that of tyrosine. This molecule is commonly found in conditions with high oxidative stress such as inflammation and is present in tissues with significant reactive nitrogen species. 3-Nitrotyrosine detection is facilitated by various techniques including nitrotyrosine staining and plays a notable role in the study of oxidative stress markers.

Biological function summary

Modification of tyrosine can affect protein function and cell signaling. This post-translational modification can hinder protein function or disrupt protein-protein interactions altering cellular processes. Nitrotyrosine often forms part of the cellular response to oxidative damage contributing to complex antioxidant defenses. The presence of nitrotyrosine indicates a cellular environment stressed by reactive nitrogen species linking it to pathophysiological processes in cells undergoing such stress.

Pathways

3-nitrotyrosine acts in oxidative stress and cell signaling routes. It integrates into pathways related to the cellular stress response particularly the antioxidant defense mechanisms. Proteins such as superoxide dismutase (SOD) may indirectly relate to nitrotyrosine since they mitigate reactive species trying to maintain redox balance. The presence of nitrotyrosine can indicate excess stress on these systems reflecting shifts in signaling that affect cell survival.

Associated diseases and disorders

High levels of 3-nitrotyrosine associate with cardiovascular diseases and neurodegenerative disorders. In diseases such as atherosclerosis and Alzheimer's 3-nitrotyrosine presence indicates elevated oxidative stress. Through these conditions it relates to proteins like amyloid-beta in Alzheimer's disease or low-density lipoprotein (LDL) in cardiovascular disease. These associations emphasize the potential of 3-nitrotyrosine as a biomarker for oxidative damage in stress-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282), expandable thumbnail

    Flow Cytometry - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282)

    HL-60 cells were stained with 1 µg/ml ab110282 following treatment with 2 mM peroxynitrite (blue) or vehicle control (red). No primary antibody control is shown in black. Peroxynitrite modifies tyrosine residues to 3-nitrotyrosine.

  • Immunocytochemistry/ Immunofluorescence - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282)

    Immunocytochemistry image of ab110282 stained Human HeLa cells (A) and fibroblast cells (B, C).
    Cells grown on slides were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Slides were treated with/without 1 mM peroxynitrite to modify exposed tyrosines to 3-nitrotyrosine. Slides were blocked and incubated with tab110282 at 2 µg/ml overnight at 4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. For reference, the mitochondria (red) were identified by HSP60 / Alexa Fluor® 594 and DAPI was used to stain the cell nuclei (blue).
    HeLa cells (A) and fibroblast cells (B) show surface modification of tyrosine to 3-nitrotyrosine after exposure to peroxynitrite. While (C) unexposed fibroblast cells show no modification.

  • Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282), expandable thumbnail

    Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282)

    All lanes: Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282) at 1 µg/mL

    Lane 1: Bovine heart mitochondria

    Lane 2: Bovine heart mitochondria - nitrated

    Lane 3: BSA

    Lane 4: BSA - nitrated

  • Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282), expandable thumbnail

    Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282)

    Prior to runnning the samples, the membrane was treated with sodium dithionite to reduce nitrotyrosine to aminotyrosine.

    All lanes: Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282) at 1 µg/mL

    Lane 1: Bovine heart mitochondria

    Lane 2: Bovine heart mitochondria - nitrated

    Lane 3: BSA

    Lane 4: BSA - nitrated

  • Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282), expandable thumbnail

    Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282)

    In this experiment, the antibody was first blocked with free nitrotyrosine before being used to blot the membrane.
    The figure shows that ab110282's binding capacity was not inhibited by the free nitrotyrosine, and so only binds to the protein-bound form.

    All lanes: Western blot - Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282) at 1 µg/mL

    Lane 1: Bovine heart mitochondria

    Lane 2: Bovine heart mitochondria - nitrated

    Lane 3: BSA

    Lane 4: BSA - nitrated

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com