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Rabbit Recombinant Monoclonal 3-Nitrotyrosine antibody. Suitable for WB, Dot, IP, I-ELISA and reacts with Modified Amino Acid samples.

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Images

Western blot - Anti-3-Nitrotyrosine antibody [EPR27217-88] (AB314438), expandable thumbnail
  • Dot Blot - Anti-3-Nitrotyrosine antibody [EPR27217-88] (AB314438), expandable thumbnail
  • Immunoprecipitation - Anti-3-Nitrotyrosine antibody [EPR27217-88] (AB314438), expandable thumbnail
  • Indirect ELISA - Anti-3-Nitrotyrosine antibody [EPR27217-88] (AB314438), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotIPI-ELISAIHC-PICC/IFFlow Cyt (Intra)
Modified Amino Acid
Tested
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Modified Amino Acid
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Modified Amino Acid
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Modified Amino Acid
Dilution info
1/30
Notes

-

Tested
Tested

Species
Modified Amino Acid
Dilution info
1000 ng/mL
Notes

-

Not recommended
Not recommended

Species
Modified Amino Acid
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Modified Amino Acid
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Modified Amino Acid
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Recommended products

Rabbit Recombinant Monoclonal 3-Nitrotyrosine antibody. Suitable for WB, Dot, IP, I-ELISA and reacts with Modified Amino Acid samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27217-88
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

3-Nitrotyrosine forms when peroxynitrite interacts with tyrosine residues in proteins. It is a marker of nitrosative stress and oxidative damage with the molecular mass of the modified residue remaining similar to that of tyrosine. This molecule is commonly found in conditions with high oxidative stress such as inflammation and is present in tissues with significant reactive nitrogen species. 3-Nitrotyrosine detection is facilitated by various techniques including nitrotyrosine staining and plays a notable role in the study of oxidative stress markers.

Biological function summary

Modification of tyrosine can affect protein function and cell signaling. This post-translational modification can hinder protein function or disrupt protein-protein interactions altering cellular processes. Nitrotyrosine often forms part of the cellular response to oxidative damage contributing to complex antioxidant defenses. The presence of nitrotyrosine indicates a cellular environment stressed by reactive nitrogen species linking it to pathophysiological processes in cells undergoing such stress.

Pathways

3-nitrotyrosine acts in oxidative stress and cell signaling routes. It integrates into pathways related to the cellular stress response particularly the antioxidant defense mechanisms. Proteins such as superoxide dismutase (SOD) may indirectly relate to nitrotyrosine since they mitigate reactive species trying to maintain redox balance. The presence of nitrotyrosine can indicate excess stress on these systems reflecting shifts in signaling that affect cell survival.

Associated diseases and disorders

High levels of 3-nitrotyrosine associate with cardiovascular diseases and neurodegenerative disorders. In diseases such as atherosclerosis and Alzheimer's 3-nitrotyrosine presence indicates elevated oxidative stress. Through these conditions it relates to proteins like amyloid-beta in Alzheimer's disease or low-density lipoprotein (LDL) in cardiovascular disease. These associations emphasize the potential of 3-nitrotyrosine as a biomarker for oxidative damage in stress-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-3-Nitrotyrosine antibody [EPR27217-88] (ab314438), expandable thumbnail

    Western blot - Anti-3-Nitrotyrosine antibody [EPR27217-88] (ab314438)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The identity of the bands around 50 kDa are unknown.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    In Western blot, Anti-phosphotyrosine antibody [EPR16871] (Anti-Phosphotyrosine antibody [EPR16871] ab179530) staining at 1/1000 dilution

    All lanes: Western blot - Anti-3-Nitrotyrosine antibody [EPR27217-88] (ab314438) at 1/1000 dilution

    Lanes 1 and 7: Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa treated with 3 mM peroxynitrite for 30 minutes whole cell lysate at 20 µg

    Lane 3: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 treated with 3 mM peroxynitrite for 30 minutes whole cell lysate at 20 µg

    Lane 5: Untreated PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Lane 6: PC-12 treated with 3 mM peroxynitrite for 30 minutes whole cell lysate at 20 µg

    Lane 8: HeLa treated with 1 mM pervanadate for 30 minutes whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 1-250 kDa

    Exposure time: 37s

  • Dot Blot - Anti-3-Nitrotyrosine antibody [EPR27217-88] (ab314438), expandable thumbnail

    Dot Blot - Anti-3-Nitrotyrosine antibody [EPR27217-88] (ab314438)

    Dot blot analysis of 3-Nitrotyrosine using ab314438 at 1:1000 (0.497 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
    Exposure time: 10 seconds
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Immunoprecipitation - Anti-3-Nitrotyrosine antibody [EPR27217-88] (ab314438), expandable thumbnail

    Immunoprecipitation - Anti-3-Nitrotyrosine antibody [EPR27217-88] (ab314438)

    3-Nitrotyrosine was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) treated with 3 mM peroxynitrite for 30 minutes whole cell lysate with ab314438 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314438 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) treated with 3 mM peroxynitrite for 30 minutes whole cell lysate
    Lane 2: ab314438 IP in HeLa (human cervix adenocarcinoma epithelial cell) treated with 3 mM peroxynitrite for 30 minutes whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314438 in HeLa treated with 3 mM peroxynitrite for 30 minutes whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-3-Nitrotyrosine antibody [EPR27217-88] (ab314438) at 1/30 dilution

    All lanes: HeLa (human cervix adenocarcinoma epithelial cell) treated with 3 mM peroxynitrite for 30 minutes whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 15s

  • Indirect ELISA - Anti-3-Nitrotyrosine antibody [EPR27217-88] (ab314438), expandable thumbnail

    Indirect ELISA - Anti-3-Nitrotyrosine antibody [EPR27217-88] (ab314438)

    Indirect ELISA analysis of abab314438 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
    Antigen: 3-Nitrotyrosine peptide，BSA-crosslinked single 3-Nitrotyrosine amino acid.
    Antigen concentration: 1000 ng/ml

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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