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Rabbit Recombinant Monoclonal 3-Nitrotyrosine antibody. Carrier free. Suitable for WB, Dot, IP, I-ELISA and reacts with Modified Amino Acid samples.

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Images

Western blot - Anti-3-Nitrotyrosine antibody [EPR27217-88] - BSA and Azide free (AB314439), expandable thumbnail
  • Dot Blot - Anti-3-Nitrotyrosine antibody [EPR27217-88] - BSA and Azide free (AB314439), expandable thumbnail
  • Immunoprecipitation - Anti-3-Nitrotyrosine antibody [EPR27217-88] - BSA and Azide free (AB314439), expandable thumbnail
  • Indirect ELISA - Anti-3-Nitrotyrosine antibody [EPR27217-88] - BSA and Azide free (AB314439), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotIPI-ELISAIHC-PICC/IFFlow Cyt (Intra)
Modified Amino Acid
Tested
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Modified Amino Acid
Dilution info
-
Notes

-

Tested
Tested

Species
Modified Amino Acid
Dilution info
-
Notes

-

Tested
Tested

Species
Modified Amino Acid
Dilution info
-
Notes

-

Tested
Tested

Species
Modified Amino Acid
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Modified Amino Acid
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Modified Amino Acid
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Modified Amino Acid
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Alternative names

3-NT, 3NT

Rabbit Recombinant Monoclonal 3-Nitrotyrosine antibody. Carrier free. Suitable for WB, Dot, IP, I-ELISA and reacts with Modified Amino Acid samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27217-88
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab314439 is the carrier-free version of Anti-3-Nitrotyrosine antibody [EPR27217-88] ab314438.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

3-Nitrotyrosine forms when peroxynitrite interacts with tyrosine residues in proteins. It is a marker of nitrosative stress and oxidative damage with the molecular mass of the modified residue remaining similar to that of tyrosine. This molecule is commonly found in conditions with high oxidative stress such as inflammation and is present in tissues with significant reactive nitrogen species. 3-Nitrotyrosine detection is facilitated by various techniques including nitrotyrosine staining and plays a notable role in the study of oxidative stress markers.

Biological function summary

Modification of tyrosine can affect protein function and cell signaling. This post-translational modification can hinder protein function or disrupt protein-protein interactions altering cellular processes. Nitrotyrosine often forms part of the cellular response to oxidative damage contributing to complex antioxidant defenses. The presence of nitrotyrosine indicates a cellular environment stressed by reactive nitrogen species linking it to pathophysiological processes in cells undergoing such stress.

Pathways

3-nitrotyrosine acts in oxidative stress and cell signaling routes. It integrates into pathways related to the cellular stress response particularly the antioxidant defense mechanisms. Proteins such as superoxide dismutase (SOD) may indirectly relate to nitrotyrosine since they mitigate reactive species trying to maintain redox balance. The presence of nitrotyrosine can indicate excess stress on these systems reflecting shifts in signaling that affect cell survival.

Associated diseases and disorders

High levels of 3-nitrotyrosine associate with cardiovascular diseases and neurodegenerative disorders. In diseases such as atherosclerosis and Alzheimer's 3-nitrotyrosine presence indicates elevated oxidative stress. Through these conditions it relates to proteins like amyloid-beta in Alzheimer's disease or low-density lipoprotein (LDL) in cardiovascular disease. These associations emphasize the potential of 3-nitrotyrosine as a biomarker for oxidative damage in stress-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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