AB223160

Anti-4-1BBL antibody [CD137L/1547]

Name: Anti-4-1BBL antibody [CD137L/1547] (ab223160) | Abcam
Brand: Abcam Limited
SKU: AB223160
Price: 510 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal 4-1BBL antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNFSF9.

View Alternative Names

Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4-1BBL antibody [CD137L/1547] (AB223160)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4-1BBL antibody [CD137L/1547] (AB223160)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for 4-1BBL with ab223160 at 2 μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4-1BBL antibody [CD137L/1547] (AB223160)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Renal Cell Carcinoma labelling 4-1BBL with ab223160 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CD137L/1547

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TNFSF9.

P41273

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 minutes at RT).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab223160 is purified from bioreactor concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 4-1BBL protein also known as CD137L or 4-1BB ligand acts mechanically as a member of the tumor necrosis factor (TNF) superfamily. The 4-1BBL with a mass of around 36 kDa exists as a type II transmembrane protein. It gets expressed on activated antigen-presenting cells like dendritic cells macrophages and B cells. This costimulatory molecule primarily interacts with its receptor 4-1BB (CD137) on T cells promoting their activation.

Biological function summary

The 4-1BBL protein plays an important role in mediating the co-stimulatory signal needed for T-cell proliferation survival and cytokine production. This protein contributes to the formation of the immunological synapse and doesn't exist alone but rather interacts directly with its receptor CD137 forming a receptor-ligand complex. The 4-1BBL-CD137 interaction strengthens signals delivered by the T-cell receptor enhancing immune responses.

Pathways

The 4-1BBL protein integrates into immune-related signaling pathways. It significantly impacts the NF-kB and MAPK pathways important for T cell survival and activation. The interaction between 4-1BBL and its related proteins such as the 4-1BB/CD137 receptor initiates downstream cascades that lead to the activation of transcription factors involved in immune response.

The 4-1BBL protein connects to conditions such as cancer and autoimmune diseases. Overexpression or dysregulation of 4-1BBL has associations with autoimmune conditions like rheumatoid arthritis due to prolonged T cell activation. In cancer 4-1BBL's interaction with CD137 has potential therapeutic implications with efforts to harness this axis to bolster anti-tumor immunity. The modulation of 4-1BBL activity can influence disease outcomes highlighting its significance as a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.

See full target information TNFSF9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:101015 PubMed37075701

2023

A kinome-wide CRISPR screen identifies CK1α as a target to overcome enzalutamide resistance of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Liu,Yue Zhao,Daheng He,Katelyn M Jones,Shan Tang,Derek B Allison,Yanquan Zhang,Jing Chen,Qiongsi Zhang,Xinyi Wang,Chaohao Li,Chi Wang,Lang Li,Xiaoqi Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-4-1BBL antibody [CD137L/1547]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4-1BBL antibody [CD137L/1547] (AB223160)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4-1BBL antibody [CD137L/1547] (AB223160)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise