AB68185

Anti-4-1BBL antibody [EPR1172Y]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal 4-1BBL antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9

4 Images

Lab

Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (AB68185)

ab68185 (purified) at 1/50 immunoprecipitating 4-1BBL in HEK293 whole cell lysate.

Lane 1 (input) : HEK293 whole cell lysate (10μg)

Lane 2 (+) : ab68185 + HEK293 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab68185 in HEK293 whole cell lysate.

For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (ab68185)

Predicted band size: 27 kDa

Observed band size: 25 kDa,32 kDa

false

Lab

Western blot - Anti-4-1BBL antibody [EPR1172Y] (AB68185)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution

All lanes:

HEK293 whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 27 kDa

Observed band size: 25-32 kDa

false

Unknown

Western blot - Anti-4-1BBL antibody [EPR1172Y] (AB68185)

All lanes:

Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/2000 dilution

All lanes:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 27 kDa

Observed band size: 25 kDa

false

Lab

Western blot - Anti-4-1BBL antibody [EPR1172Y] (AB68185)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-4-1BBL antibody [EPR1172Y] (ab68185) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 27 kDa

Observed band size: 25 kDa,32 kDa

false

Carrier free

Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free
HRP

HRP Anti-4-1BBL antibody [EPR1172Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1172Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/50", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 4-1BBL protein also known as CD137L or 4-1BB ligand acts mechanically as a member of the tumor necrosis factor (TNF) superfamily. The 4-1BBL with a mass of around 36 kDa exists as a type II transmembrane protein. It gets expressed on activated antigen-presenting cells like dendritic cells macrophages and B cells. This costimulatory molecule primarily interacts with its receptor 4-1BB (CD137) on T cells promoting their activation.

Biological function summary

The 4-1BBL protein plays an important role in mediating the co-stimulatory signal needed for T-cell proliferation survival and cytokine production. This protein contributes to the formation of the immunological synapse and doesn't exist alone but rather interacts directly with its receptor CD137 forming a receptor-ligand complex. The 4-1BBL-CD137 interaction strengthens signals delivered by the T-cell receptor enhancing immune responses.

Pathways

The 4-1BBL protein integrates into immune-related signaling pathways. It significantly impacts the NF-kB and MAPK pathways important for T cell survival and activation. The interaction between 4-1BBL and its related proteins such as the 4-1BB/CD137 receptor initiates downstream cascades that lead to the activation of transcription factors involved in immune response.

The 4-1BBL protein connects to conditions such as cancer and autoimmune diseases. Overexpression or dysregulation of 4-1BBL has associations with autoimmune conditions like rheumatoid arthritis due to prolonged T cell activation. In cancer 4-1BBL's interaction with CD137 has potential therapeutic implications with efforts to harness this axis to bolster anti-tumor immunity. The modulation of 4-1BBL activity can influence disease outcomes highlighting its significance as a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.

See full target information TNFSF9

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 162:167 PubMed40830812

2025

RBM15 enhances paclitaxel resistance in triple-negative breast cancer by targeting mA methylation of TNFSF9 and inducing polarization of tumor-associated macrophages to M2 phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Jinkun Fu,Chao Wei,Yijian Chen,Xiaoming He,Kun Zhang

Materials today. Bio 34:102160 PubMed40822925

2025

Delivered baicalein immunomodulatory hydrogel with dual properties of pH-responsive and anti-infection orchestrates pro-regenerative response of macrophages for enhanced hypertrophic scars therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Fengqing Shang,Yanling Qu,Yu Li,Lingjuan Dong,Dan Liu,Zhe Wang,Afeng Li,Yinghui Li,Dan Zhang,Leiguo Ming,Ronghua Jin

International journal of molecular sciences 24: PubMed37373140

2023

In Vitro and In Vivo Efficacy of a Stroma-Targeted, Tumor Microenvironment Responsive Oncolytic Adenovirus in Different Preclinical Models of Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Alfano,Eduardo G A Cafferata,Mariela Gangemi,Alejandro Nicola Candia,Cristian M Malnero,Ismael Bermudez,Mauricio Vargas Lopez,Gregorio David Ríos,Cecilia Rotondaro,Nicasio Cuneo,David T Curiel,Osvaldo L Podhajcer,Maria Veronica Lopez

International journal of molecular sciences 23: PubMed36142828

2022

A Novel L-Phenylalanine Dipeptide Inhibits the Growth and Metastasis of Prostate Cancer Cells via Targeting DUSP1 and TNFSF9.

Applications

Unspecified application

Species

Unspecified reactive species

Lanlan Li,Mingfei Yang,Jia Yu,Sha Cheng,Mashaal Ahmad,Caihong Wu,Xinwei Wan,Bixue Xu,Yaacov Ben-David,Heng Luo

The Journal of experimental medicine 219: PubMed35657354

2022

Inherited TNFSF9 deficiency causes broad Epstein-Barr virus infection with EBV+ smooth muscle tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Fournier,Akihiro Hoshino,Julie Bruneau,Camille Bachelet,Mathieu Fusaro,Roman Klifa,Romain Lévy,Christelle Lenoir,Claire Soudais,Capucine Picard,Stéphane Blanche,Martin Castelle,Despina Moshous,Thierry Molina,Anne-Sophie Defachelles,Bénédicte Neven,Sylvain Latour

Respiratory research 22:52 PubMed33579290

2021

Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Fu,Chunyan Yang,Bing Chen,Kexing Zeng,Siyuan Chen,Yi Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-4-1BBL antibody [EPR1172Y]

Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (AB68185)

Western blot - Anti-4-1BBL antibody [EPR1172Y] (AB68185)

Western blot - Anti-4-1BBL antibody [EPR1172Y] (AB68185)

Western blot - Anti-4-1BBL antibody [EPR1172Y] (AB68185)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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