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AB247373

Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal 4-1BBL antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9

4 Images
Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)
  • IP

Lab

Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)

This data was developed using ab68185, the same antibody clone in a different buffer formulation.

ab68185 (purified) at 1/50 immunoprecipitating 4-1BBL in HEK293 whole cell lysate.

Lane 1 (input) : HEK293 whole cell lysate (10μg)

Lane 2 (+) : ab68185 + HEK293 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab68185 in HEK293 whole cell lysate.

For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (<a href='/en-us/products/primary-antibodies/4-1bbl-antibody-epr1172y-ab68185'>ab68185</a>)

Predicted band size: 27 kDa

Observed band size: 25 kDa,32 kDa

false

Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)
  • WB

Lab

Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)

This data was developed using ab68185, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-4-1BBL antibody [EPR1172Y] (<a href='/en-us/products/primary-antibodies/4-1bbl-antibody-epr1172y-ab68185'>ab68185</a>) at 1/1000 dilution

All lanes:

HEK293 whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 27 kDa

Observed band size: 25-32 kDa

false

Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)
  • WB

Unknown

Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)

This data was developed using ab68185, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-4-1BBL antibody [EPR1172Y] (<a href='/en-us/products/primary-antibodies/4-1bbl-antibody-epr1172y-ab68185'>ab68185</a>) at 1/2000 dilution

All lanes:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 27 kDa

Observed band size: 25 kDa

false

Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)
  • WB

Lab

Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)

This data was developed using ab68185, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-4-1BBL antibody [EPR1172Y] (<a href='/en-us/products/primary-antibodies/4-1bbl-antibody-epr1172y-ab68185'>ab68185</a>) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 27 kDa

Observed band size: 25 kDa,32 kDa

false

  • Unconjugated

    Anti-4-1BBL antibody [EPR1172Y]

  • HRP

    HRP Anti-4-1BBL antibody [EPR1172Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1172Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Recombinant|SimpleStep

ELISA - Human 4-1BBL ELISA Kit (AB246536)

  • 0
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  • 0
  • 0
Primary Antibodies

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Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

  • 5
  • 17
Primary Antibodies

AB251600

Anti-GITR antibody [CAL52] - BSA and Azide free

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Immunocytochemistry/ Immunofluorescence - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600)

  • 0
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Primary Antibodies

AB243648

Anti-CD137 antibody [EPR23218-111]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] (AB243648)

  • 0
  • 0
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Reactivity data

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Product details

ab247373 is the carrier-free version of ab68185.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 4-1BBL protein also known as CD137L or 4-1BB ligand acts mechanically as a member of the tumor necrosis factor (TNF) superfamily. The 4-1BBL with a mass of around 36 kDa exists as a type II transmembrane protein. It gets expressed on activated antigen-presenting cells like dendritic cells macrophages and B cells. This costimulatory molecule primarily interacts with its receptor 4-1BB (CD137) on T cells promoting their activation.
Biological function summary

The 4-1BBL protein plays an important role in mediating the co-stimulatory signal needed for T-cell proliferation survival and cytokine production. This protein contributes to the formation of the immunological synapse and doesn't exist alone but rather interacts directly with its receptor CD137 forming a receptor-ligand complex. The 4-1BBL-CD137 interaction strengthens signals delivered by the T-cell receptor enhancing immune responses.

Pathways

The 4-1BBL protein integrates into immune-related signaling pathways. It significantly impacts the NF-kB and MAPK pathways important for T cell survival and activation. The interaction between 4-1BBL and its related proteins such as the 4-1BB/CD137 receptor initiates downstream cascades that lead to the activation of transcription factors involved in immune response.

The 4-1BBL protein connects to conditions such as cancer and autoimmune diseases. Overexpression or dysregulation of 4-1BBL has associations with autoimmune conditions like rheumatoid arthritis due to prolonged T cell activation. In cancer 4-1BBL's interaction with CD137 has potential therapeutic implications with efforts to harness this axis to bolster anti-tumor immunity. The modulation of 4-1BBL activity can influence disease outcomes highlighting its significance as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
See full target information TNFSF9
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com