Rabbit Recombinant Monoclonal 4-1BBL antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373), expandable thumbnail](https://content.abcam.com/products/images/4-1bbl-antibody-epr1172y-bsa-and-azide-free-ab247373--western-blot-img83302.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)")
![Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373), expandable thumbnail](https://content.abcam.com/products/images/4-1bbl-antibody-epr1172y-bsa-and-azide-free-ab247373--immunoprecipitation-img20860.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)")
![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373), expandable thumbnail](https://content.abcam.com/products/images/4-1bbl-antibody-epr1172y-bsa-and-azide-free-ab247373--western-blot-img143070.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)")
![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373), expandable thumbnail](https://content.abcam.com/products/images/4-1bbl-antibody-epr1172y-bsa-and-azide-free-ab247373--western-blot-img21485.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (AB247373)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
Alternative names
Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9
Recommended products
Rabbit Recombinant Monoclonal 4-1BBL antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1172Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247373 is the carrier-free version of Anti-4-1BBL antibody [EPR1172Y] ab68185.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 4-1BBL protein also known as CD137L or 4-1BB ligand acts mechanically as a member of the tumor necrosis factor (TNF) superfamily. The 4-1BBL with a mass of around 36 kDa exists as a type II transmembrane protein. It gets expressed on activated antigen-presenting cells like dendritic cells macrophages and B cells. This costimulatory molecule primarily interacts with its receptor 4-1BB (CD137) on T cells promoting their activation.
- Biological function summary
The 4-1BBL protein plays an important role in mediating the co-stimulatory signal needed for T-cell proliferation survival and cytokine production. This protein contributes to the formation of the immunological synapse and doesn't exist alone but rather interacts directly with its receptor CD137 forming a receptor-ligand complex. The 4-1BBL-CD137 interaction strengthens signals delivered by the T-cell receptor enhancing immune responses.
- Pathways
The 4-1BBL protein integrates into immune-related signaling pathways. It significantly impacts the NF-kB and MAPK pathways important for T cell survival and activation. The interaction between 4-1BBL and its related proteins such as the 4-1BB/CD137 receptor initiates downstream cascades that lead to the activation of transcription factors involved in immune response.
- Associated diseases and disorders
The 4-1BBL protein connects to conditions such as cancer and autoimmune diseases. Overexpression or dysregulation of 4-1BBL has associations with autoimmune conditions like rheumatoid arthritis due to prolonged T cell activation. In cancer 4-1BBL's interaction with CD137 has potential therapeutic implications with efforts to harness this axis to bolster anti-tumor immunity. The modulation of 4-1BBL activity can influence disease outcomes highlighting its significance as a target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373), expandable thumbnail](https://content.abcam.com/products/images/4-1bbl-antibody-epr1172y-bsa-and-azide-free-ab247373--western-blot-img83302.jpg "Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)")
Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
This data was developed using Anti-4-1BBL antibody [EPR1172Y] ab68185, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-4-1BBL antibody [EPR1172Y] (Anti-4-1BBL antibody [EPR1172Y] ab68185) at 1/1000 dilution
All lanes: Raji whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 25 kDa, 32 kDa
![Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373), expandable thumbnail](https://content.abcam.com/products/images/4-1bbl-antibody-epr1172y-bsa-and-azide-free-ab247373--immunoprecipitation-img20860.jpg "Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)")
Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
This data was developed using Anti-4-1BBL antibody [EPR1172Y] ab68185, the same antibody clone in a different buffer formulation.
Anti-4-1BBL antibody [EPR1172Y] ab68185 (purified) at 1/50 immunoprecipitating 4-1BBL in HEK293 whole cell lysate.
Lane 1 (input): HEK293 whole cell lysate (10μg)
Lane 2 (+): Anti-4-1BBL antibody [EPR1172Y] ab68185 + HEK293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-4-1BBL antibody [EPR1172Y] ab68185 in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (Anti-4-1BBL antibody [EPR1172Y] ab68185)
Predicted band size: 27 kDa
Observed band size: 25 kDa, 32 kDa
![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373), expandable thumbnail](https://content.abcam.com/products/images/4-1bbl-antibody-epr1172y-bsa-and-azide-free-ab247373--western-blot-img143070.jpg "Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)")
Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
This data was developed using Anti-4-1BBL antibody [EPR1172Y] ab68185, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-4-1BBL antibody [EPR1172Y] (Anti-4-1BBL antibody [EPR1172Y] ab68185) at 1/1000 dilution
All lanes: HEK293 whole cell lysate at 20 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 27 kDa
Observed band size: 25-32 kDa
![Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373), expandable thumbnail](https://content.abcam.com/products/images/4-1bbl-antibody-epr1172y-bsa-and-azide-free-ab247373--western-blot-img21485.jpg "Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)")
Western blot - Anti-4-1BBL antibody [EPR1172Y] - BSA and Azide free (ab247373)
This data was developed using Anti-4-1BBL antibody [EPR1172Y] ab68185, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-4-1BBL antibody [EPR1172Y] (Anti-4-1BBL antibody [EPR1172Y] ab68185) at 1/2000 dilution
All lanes: 293T cell lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 25 kDa
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