AB6034

Anti-4E-T antibody

Name: Anti-4E-T antibody (ab6034) | Abcam
Brand: Abcam Limited
SKU: AB6034
Price: 525 USD
Availability: InStock

(1 Review)

(4 Publications)

Goat Polyclonal 4E-T antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4ENIF1 aa 950 to C-terminus.

View Alternative Names

Eukaryotic translation initiation factor 4E transporter, 4E-T, eIF4E transporter, Eukaryotic translation initiation factor 4E nuclear import factor 1, EIF4ENIF1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4E-T antibody (AB6034)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4E-T antibody (AB6034)

ab6034 at 5ug/ml staining 4E-T in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

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Western blot - Anti-4E-T antibody (AB6034)

ab6034 at 0.25μg/ml staining approximately 140-150kDa band of 4E-T in HEK293 Lysate by Western blot (ECL). ab6034 at 0.25μg/ml staining approximately 140-150kDa band of EIF4ENIF1 in HEK293 Lysate by Western blot (ECL).

All lanes:

Western blot - Anti-4E-T antibody (ab6034)

Predicted band size: 108 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human EIF4ENIF1 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NRA8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-0.5 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. The predicted molecular weight is 108 kDa according to NP_062817, however our observation agrees with that of Dostie et al, PMID: 10856257.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 4E-T protein also known as eIF4E-Transporter plays a critical role in translational regulation by controlling the localization and function of eIF4E an important component of the mRNA cap-binding complex. The mass of 4E-T is approximately 103 kDa. It is expressed in various tissues including the brain and testis suggesting its involvement in diverse physiological processes. The protein collaborates with eIF4E to facilitate or repress translation of specific mRNAs affecting gene expression at the translational level.

Biological function summary

4E-T regulates mRNA metabolism and translation serving as a modulator within the cell. It forms a complex with eIF4E and other translation repressors such as DEAD-box helicase DDX6 and Pat1 protein which then associates with mRNA to influence its translational state. This interaction enables 4E-T to impact gene expression by selectively binding and sequestering capped mRNAs therefore blocking their translation initiation at ribosomes.

Pathways

4E-T is involved in the mTOR signaling pathway which is essential for cell growth and metabolism. It also plays a role in the stress response pathway known as the nonsense-mediated mRNA decay pathway. In these pathways 4E-T interacts with proteins like LSm1 and UPF1 coordinating the cellular response to nutrient availability and stress and contributing to the degradation of faulty mRNA.

4E-T has been implicated in cancer and neurodegenerative diseases. In cancer its regulation of mRNA translation and interaction with proteins like eIF4G can affect tumor cell growth and survival. In neurodegenerative diseases alterations in its regulation can influence neuron health with its association with proteins like CPEB impacting synaptic plasticity and memory. Understanding 4E-T's involvement in these diseases provides avenues for potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies) (PubMed : 16157702, PubMed : 24335285, PubMed : 27342281, PubMed : 32354837). Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed : 24335285, PubMed : 32354837). Acts as a binding platform for multiple RNA-binding proteins : promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation (PubMed : 27342281, PubMed : 32354837). Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity). Promotes miRNA-mediated translational repression (PubMed : 24335285, PubMed : 27342281, PubMed : 28487484). Required for the formation of P-bodies (PubMed : 16157702, PubMed : 22966201, PubMed : 27342281, PubMed : 32354837). Involved in mRNA translational repression mediated by the miRNA effector TNRC6B by protecting TNRC6B-targeted mRNAs from decapping and subsequent decay (PubMed : 32354837). Also acts as a nucleoplasmic shuttling protein, which mediates the nuclear import of EIF4E and DDX6 by a piggy-back mechanism (PubMed : 10856257, PubMed : 28216671).

See full target information EIF4ENIF1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 95:e0092221 PubMed34287048

2021

Enterovirus 71 2A Protease Inhibits P-Body Formation To Promote Viral RNA Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Fan,Zihang Xu,Pengfei Liu,Yali Qin,Mingzhou Chen

Nucleic acids research 44:6318-34 PubMed27342281

2016

The DDX6-4E-T interaction mediates translational repression and P-body assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasiia Kamenska,Clare Simpson,Caroline Vindry,Helen Broomhead,Marianne Bénard,Michèle Ernoult-Lange,Benjamin P Lee,Lorna W Harries,Dominique Weil,Nancy Standart

RNA (New York, N.Y.) 21:1335-45 PubMed26015597

2015

Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Shruti Waghray,Clay Williams,Joshua J Coon,Marvin Wickens

Molecular biology of the cell 23:213-24 PubMed22090346

2011

RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor.

Applications

ICC/IF

Species

Human

Aline Marnef,Dominique Weil,Nancy Standart

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-4E-T antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4E-T antibody (AB6034)

Western blot - Anti-4E-T antibody (AB6034)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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