Goat Polyclonal 4E-T antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4ENIF1 aa 950 to C-terminus.
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Publications
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- Journal of virology 95:e00922212021Enterovirus 71 2A Protease Inhibits P-Body Formation To Promote Viral RNA Synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanshan Fan,Zihang Xu,Pengfei Liu,Yali Qin,Mingzhou ChenPubMed 34287048
- Nucleic acids research 44:6318-342016The DDX6-4E-T interaction mediates translational repression and P-body assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnastasiia Kamenska et. al.PubMed 27342281
- RNA (New York, N.Y.) 21:1335-452015Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShruti Waghray et. al.PubMed 26015597
- Molecular biology of the cell 23:213-242011RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human EIF4ENIF1 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRA8
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-0.50000 µg/mL | Notes A 1 hour primary incubation is recommended for this product. The predicted molecular weight is 108 kDa according to NP_062817, however our observation agrees with that of Dostie et al, PMID: 10856257. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:27342281, PubMed:32354837). Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:24335285, PubMed:32354837). Acts as a binding platform for multiple RNA-binding proteins: promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation (PubMed:27342281, PubMed:32354837). Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity). Promotes miRNA-mediated translational repression (PubMed:24335285, PubMed:27342281, PubMed:28487484). Required for the formation of P-bodies (PubMed:16157702, PubMed:22966201, PubMed:27342281, PubMed:32354837). Involved in mRNA translational repression mediated by the miRNA effector TNRC6B by protecting TNRC6B-targeted mRNAs from decapping and subsequent decay (PubMed:32354837). Also acts as a nucleoplasmic shuttling protein, which mediates the nuclear import of EIF4E and DDX6 by a piggy-back mechanism (PubMed:10856257, PubMed:28216671).
Alternative names
Eukaryotic translation initiation factor 4E transporter, 4E-T, eIF4E transporter, Eukaryotic translation initiation factor 4E nuclear import factor 1, EIF4ENIF1
Recommended products
Goat Polyclonal 4E-T antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4ENIF1 aa 950 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human EIF4ENIF1 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRA8
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 4E-T protein also known as eIF4E-Transporter plays a critical role in translational regulation by controlling the localization and function of eIF4E an important component of the mRNA cap-binding complex. The mass of 4E-T is approximately 103 kDa. It is expressed in various tissues including the brain and testis suggesting its involvement in diverse physiological processes. The protein collaborates with eIF4E to facilitate or repress translation of specific mRNAs affecting gene expression at the translational level.
- Biological function summary
4E-T regulates mRNA metabolism and translation serving as a modulator within the cell. It forms a complex with eIF4E and other translation repressors such as DEAD-box helicase DDX6 and Pat1 protein which then associates with mRNA to influence its translational state. This interaction enables 4E-T to impact gene expression by selectively binding and sequestering capped mRNAs therefore blocking their translation initiation at ribosomes.
- Pathways
4E-T is involved in the mTOR signaling pathway which is essential for cell growth and metabolism. It also plays a role in the stress response pathway known as the nonsense-mediated mRNA decay pathway. In these pathways 4E-T interacts with proteins like LSm1 and UPF1 coordinating the cellular response to nutrient availability and stress and contributing to the degradation of faulty mRNA.
- Associated diseases and disorders
4E-T has been implicated in cancer and neurodegenerative diseases. In cancer its regulation of mRNA translation and interaction with proteins like eIF4G can affect tumor cell growth and survival. In neurodegenerative diseases alterations in its regulation can influence neuron health with its association with proteins like CPEB impacting synaptic plasticity and memory. Understanding 4E-T's involvement in these diseases provides avenues for potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-4E-T antibody (ab6034)
ab6034 at 0.25μg/ml staining approximately 140-150kDa band of 4E-T in HEK293 Lysate by Western blot (ECL). ab6034 at 0.25μg/ml staining approximately 140-150kDa band of EIF4ENIF1 in HEK293 Lysate by Western blot (ECL).
All lanes: Western blot - Anti-4E-T antibody (ab6034)
Predicted band size: 108 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4E-T antibody (ab6034)
ab6034 at 5ug/ml staining 4E-T in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
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