AB95030

Anti-4E-T antibody

Name: Anti-4E-T antibody (ab95030) | Abcam
Brand: Abcam Limited
SKU: AB95030

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(3 Publications)

Rabbit Polyclonal 4E-T antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4ENIF1 aa 900 to C-terminus.

View Alternative Names

Eukaryotic translation initiation factor 4E transporter, 4E-T, eIF4E transporter, Eukaryotic translation initiation factor 4E nuclear import factor 1, EIF4ENIF1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4E-T antibody (AB95030)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4E-T antibody (AB95030)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling 4E-T with ab95030 at 1/1000 (1μg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-4E-T antibody (AB95030)

Detection of 4E-T in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab95030 at 3 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-4E-T antibody (ab95030)

Predicted band size: 108 kDa

false

Unknown

Western blot - Anti-4E-T antibody (AB95030)

All lanes:

Western blot - Anti-4E-T antibody (ab95030) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

Mouse NIH3T3 whole cell lysate at 50 µg

Predicted band size: 108 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human EIF4ENIF1 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NRA8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Platypus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Turkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 4E-T protein also known as eIF4E-Transporter plays a critical role in translational regulation by controlling the localization and function of eIF4E an important component of the mRNA cap-binding complex. The mass of 4E-T is approximately 103 kDa. It is expressed in various tissues including the brain and testis suggesting its involvement in diverse physiological processes. The protein collaborates with eIF4E to facilitate or repress translation of specific mRNAs affecting gene expression at the translational level.

Biological function summary

4E-T regulates mRNA metabolism and translation serving as a modulator within the cell. It forms a complex with eIF4E and other translation repressors such as DEAD-box helicase DDX6 and Pat1 protein which then associates with mRNA to influence its translational state. This interaction enables 4E-T to impact gene expression by selectively binding and sequestering capped mRNAs therefore blocking their translation initiation at ribosomes.

Pathways

4E-T is involved in the mTOR signaling pathway which is essential for cell growth and metabolism. It also plays a role in the stress response pathway known as the nonsense-mediated mRNA decay pathway. In these pathways 4E-T interacts with proteins like LSm1 and UPF1 coordinating the cellular response to nutrient availability and stress and contributing to the degradation of faulty mRNA.

4E-T has been implicated in cancer and neurodegenerative diseases. In cancer its regulation of mRNA translation and interaction with proteins like eIF4G can affect tumor cell growth and survival. In neurodegenerative diseases alterations in its regulation can influence neuron health with its association with proteins like CPEB impacting synaptic plasticity and memory. Understanding 4E-T's involvement in these diseases provides avenues for potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies) (PubMed : 16157702, PubMed : 24335285, PubMed : 27342281, PubMed : 32354837). Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed : 24335285, PubMed : 32354837). Acts as a binding platform for multiple RNA-binding proteins : promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation (PubMed : 27342281, PubMed : 32354837). Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity). Promotes miRNA-mediated translational repression (PubMed : 24335285, PubMed : 27342281, PubMed : 28487484). Required for the formation of P-bodies (PubMed : 16157702, PubMed : 22966201, PubMed : 27342281, PubMed : 32354837). Involved in mRNA translational repression mediated by the miRNA effector TNRC6B by protecting TNRC6B-targeted mRNAs from decapping and subsequent decay (PubMed : 32354837). Also acts as a nucleoplasmic shuttling protein, which mediates the nuclear import of EIF4E and DDX6 by a piggy-back mechanism (PubMed : 10856257, PubMed : 28216671).

See full target information EIF4ENIF1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4792 PubMed40410171

2025

RNA-binding proteins DND1 and NANOS3 cooperatively suppress the entry of germ cell lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqi Wang,Honglin Yu,Zhaoyu Gu,Xiaohui Shi,Jiayue Ma,Qizhe Shao,Yao Yao,Shuo Yao,Yan Xu,Yashi Gu,Jiayue Dai,Qi Liu,Jingyan Shi,Rujie Qi,Yue Jin,Yuqian Liu,Xinchen Shen,Wenwen Huang,Heng-Jia Liu,Min Jin,Wanlu Liu,Matthew Brook,Di Chen

Journal of biomedical science 32:27 PubMed39972499

2025

The zinc finger protein ZFP36L2 inhibits flavivirus infection via the 5'-3' XRN1-mediated RNA decay pathway in the replication complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Ren-Jye Lin,Li-Hsiung Lin,Zih-Ping Chen,Bing-Cheng Liu,Pin-Chen Ko,Ching-Len Liao

Journal of cellular biochemistry 120:17337-17344 PubMed31209945

2019

Truncated TEAD-binding protein of TAZ inhibits glioma survival through the induction of apoptosis and repression of epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhao,Li-Wen Li,Rui-Feng Tian,Qiu-Feng Dong,Peng-Qi Li,Zhi-Feng Yan,Xin Yang,Jun-Li Huo,Zhou Fei,Hai-Ning Zhen

View all publications

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Product promise

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Anti-4E-T antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4E-T antibody (AB95030)

Immunoprecipitation - Anti-4E-T antibody (AB95030)

Western blot - Anti-4E-T antibody (AB95030)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise