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Rabbit Recombinant Monoclonal TAU antibody. Suitable for IP, WB, IHC-P and reacts with Rat, Mouse, Human, Recombinant fragment - Human samples. Cited in 2 publications.

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Images

Immunoprecipitation - Anti-4R Tau antibody [EPR21725] (AB218314), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (AB218314), expandable thumbnail
  • Western blot - Anti-4R Tau antibody [EPR21725] (AB218314), expandable thumbnail
  • Western blot - Anti-4R Tau antibody [EPR21725] (AB218314), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (AB218314), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PIHC-Fr
Human
Expected
Tested
Tested
Not recommended
Mouse
Expected
Tested
Tested
Not recommended
Rat
Tested
Tested
Tested
Not recommended
Recombinant fragment - Human
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Rat

Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human

Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Mouse

Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Rat

Dilution info
1/1000
Notes

-

Species

Human

Dilution info
1/1000
Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Rat

Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Human

Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Targets

MAPT, TAU_HUMAN

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TAU antibody. Suitable for IP, WB, IHC-P and reacts with Rat, Mouse, Human, Recombinant fragment - Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21725

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

4R Tau also known as the 4-repeat tau protein plays a significant role in the stabilization of microtubules in neurons. This protein appears mainly in the human central nervous system where it assists in axonal structure and transport. 4R Tau has a molecular weight of approximately 65 kDa. The expression of 4R Tau occurs mostly in neurons although its presence can also be detected in other cell types within the brain.

Biological function summary

4R Tau is a component essential for the assembly and maintenance of neuronal microtubules. It regulates microtubule dynamics by binding and enhancing their stability important for maintaining cellular and axonal integrity. In mature neurons 4R Tau exists as part of a complex with tubulin forming an important element for axonal morphology. Its function extends further into modulating neuronal growth and signal transmission which directly impacts neural connectivity.

Pathways

4R Tau is integral within the microtubule-associated protein tau (MAPT) signaling pathways. It interacts with other proteins that influence microtubule assembly and stability such as tubulin and MAP2 playing a significant role in cytoskeletal development. Additionally 4R Tau is involved in the protein folding pathways regulated by chaperones like Heat Shock Protein 70 (Hsp70) which affect its aggregation and function within neural cells.

Associated diseases and disorders

4R Tau is closely associated with neurodegenerative conditions such as Alzheimer's disease and frontotemporal dementia. Aberrant phosphorylation and aggregation of 4R Tau drive pathological tau tangles which disrupt neural networks and lead to cognitive decline. In Alzheimer’s the protein's dysfunction is linked with amyloid-beta peptide which accelerates tau pathology progression. Similarly in frontotemporal dementia 4R Tau's abnormal activity is influenced by interactions with proteins such as TDP-43 highlighting its central role in these neurodegenerative disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-4R Tau antibody [EPR21725] (ab218314), expandable thumbnail

    Immunoprecipitation - Anti-4R Tau antibody [EPR21725] (ab218314)

    4R Tau was immunoprecipitated from 0.35 mg rat hippocampus lysate 10 μg with ab218314 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab218314 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Rat hippocampus lysate 10 μg

    Lane 2: ab218314 IP in rat hippocampus lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab218314 in rat hippocampus lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 s

    All lanes: Immunoprecipitation - Anti-4R Tau antibody [EPR21725] (ab218314)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on myenteric plexus of mouse colon is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-4R Tau antibody [EPR21725] (ab218314), expandable thumbnail

    Western blot - Anti-4R Tau antibody [EPR21725] (ab218314)

    Negative control: Mouse stomach PMID:8752131; PMID:11727254.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-4: 3 minutes; Lanes 5: 37 seconds.

    All lanes: Western blot - Anti-4R Tau antibody [EPR21725] (ab218314) at 1/1000 dilution

    Lane 1: Human hippocampus lysate at 20 µg

    Lane 2: Mouse hippocampus lysate at 20 µg

    Lane 3: Mouse brain lysate at 20 µg

    Lane 4: Mouse stomach lysate at 20 µg

    Lane 5: Rat hippocampus lysate at 20 µg

    Secondary

    Lane 1: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Lanes 2 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 79 kDa

  • Western blot - Anti-4R Tau antibody [EPR21725] (ab218314), expandable thumbnail

    Western blot - Anti-4R Tau antibody [EPR21725] (ab218314)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody specifically recognizes 0N4R, 1N4R and 2N4R tau recombinant proteins. The lower bands maybe degraded tau fragments (PMID:28045602).

    All lanes: Western blot - Anti-4R Tau antibody [EPR21725] (ab218314) at 1/1000 dilution

    Lane 1: His-tagged human 2N3R Tau recombinant protein (aa1-410) 10 ng

    Lane 2: His-tagged human 2N4R Tau recombinant protein (aa1-441) 10ng

    Lane 3: His-tagged human 0N3R Tau recombinant protein (aa1-352) 10ng

    Lane 4: His-tagged human 0N4R Tau recombinant protein (aa1-383) 10ng

    Lane 5: His-tagged human 1N3R Tau recombinant protein (aa1-381) 10ng

    Lane 6: His-tagged human 1N4R Tau recombinant protein (aa1-412) 10ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 79 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on myenteric plexus of rat colon is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat cerebral cortex is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on mouse cerebrum (PMID: 18925637) is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human breast carcinoma (PMID: 15914550) is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human cerebrum is observed. Counterstained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

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