Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Rat, Mouse, Human, Recombinant fragment - Human samples.
Images
![Immunoprecipitation - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (AB242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunoprecipitation-img20209.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (AB242333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (AB242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102171.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (AB242333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (AB242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img47837.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (AB242333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (AB242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128827.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (AB242333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (AB242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134415.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (AB242333)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | WB | IHC-Fr | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Not recommended |
| Mouse | Expected | Tested | Tested | Not recommended |
| Rat | Tested | Tested | Tested | Not recommended |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Mouse, Human, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Rat, Mouse, Human, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21725
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab242333 is the carrier-free version of Anti-4R Tau antibody [EPR21725] ab218314.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
4R Tau also known as the 4-repeat tau protein plays a significant role in the stabilization of microtubules in neurons. This protein appears mainly in the human central nervous system where it assists in axonal structure and transport. 4R Tau has a molecular weight of approximately 65 kDa. The expression of 4R Tau occurs mostly in neurons although its presence can also be detected in other cell types within the brain.
- Biological function summary
4R Tau is a component essential for the assembly and maintenance of neuronal microtubules. It regulates microtubule dynamics by binding and enhancing their stability important for maintaining cellular and axonal integrity. In mature neurons 4R Tau exists as part of a complex with tubulin forming an important element for axonal morphology. Its function extends further into modulating neuronal growth and signal transmission which directly impacts neural connectivity.
- Pathways
4R Tau is integral within the microtubule-associated protein tau (MAPT) signaling pathways. It interacts with other proteins that influence microtubule assembly and stability such as tubulin and MAP2 playing a significant role in cytoskeletal development. Additionally 4R Tau is involved in the protein folding pathways regulated by chaperones like Heat Shock Protein 70 (Hsp70) which affect its aggregation and function within neural cells.
- Associated diseases and disorders
4R Tau is closely associated with neurodegenerative conditions such as Alzheimer's disease and frontotemporal dementia. Aberrant phosphorylation and aggregation of 4R Tau drive pathological tau tangles which disrupt neural networks and lead to cognitive decline. In Alzheimer’s the protein's dysfunction is linked with amyloid-beta peptide which accelerates tau pathology progression. Similarly in frontotemporal dementia 4R Tau's abnormal activity is influenced by interactions with proteins such as TDP-43 highlighting its central role in these neurodegenerative disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunoprecipitation-img20209.jpg "Immunoprecipitation - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)")
Immunoprecipitation - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)
4R Tau was immunoprecipitated from 0.35 mg rat hippocampus lysate 10 μg with Anti-4R Tau antibody [EPR21725] ab218314 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-4R Tau antibody [EPR21725] ab218314 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: rat hippocampus lysate 10 μg
Lane 2: Anti-4R Tau antibody [EPR21725] ab218314 IP in rat hippocampus lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-4R Tau antibody [EPR21725] ab218314 in rat hippocampus lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8s
This data was developed using the same antibody clone in a different buffer formuation containing PBS, BSA, glycerol, and sodium azide (Anti-4R Tau antibody [EPR21725] ab218314).
All lanes: Immunoprecipitation - Anti-4R Tau antibody [EPR21725] (Anti-4R Tau antibody [EPR21725] ab218314)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102171.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling 4R Tau with Anti-4R Tau antibody [EPR21725] ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on myenteric plexus of mouse colon is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img47837.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling 4R Tau with Anti-4R Tau antibody [EPR21725] ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on myenteric plexus of rat colon is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128827.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling 4R Tau with Anti-4R Tau antibody [EPR21725] ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat cerebral cortex is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134415.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling 4R Tau with Anti-4R Tau antibody [EPR21725] ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on mouse cerebrum (PMID: 18925637) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67827.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 4R Tau with Anti-4R Tau antibody [EPR21725] ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human breast carcinoma (PMID: 15914550) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15971.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling 4R Tau with Anti-4R Tau antibody [EPR21725] ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human cerebrum is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67827.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333), expandable thumbnail](https://content.abcam.com/products/images/4r-tau-antibody-epr21725-bsa-and-azide-free-ab242333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15971.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] - BSA and Azide free (ab242333)")
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