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AB231801

Anti-5-carboxylcytosine (5-caC) antibody

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(1 Publication)

Rabbit Polyclonal 5-carboxylcytosine (5-caC) antibody. Suitable for ICC/IF, IP, Dot and reacts with Transfected cell line - Mouse, Nucleic Acid, Synthetic peptide samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to 5-carboxylcytosine (5-caC).
2 Images
Immunoprecipitation - Anti-5-carboxylcytosine (5-caC) antibody (AB231801)
  • IP

Supplier Data

Immunoprecipitation - Anti-5-carboxylcytosine (5-caC) antibody (AB231801)

Immunoprecipitation was performed with ab231801 on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring fnger protein) gene) containing different cytosine modifcations.

The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purifed Tet2. The IP'd DNA was subsequently anaysed by qPCR using primers specifc for the control DNA fragments and for GAPDH, used as a negative control. Image shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control.

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Immunoprecipitation - Anti-5-carboxylcytosine (5-caC) antibody (ab231801)

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Dot Blot - Anti-5-carboxylcytosine (5-caC) antibody (AB231801)
  • Dot

Supplier Data

Dot Blot - Anti-5-carboxylcytosine (5-caC) antibody (AB231801)

To demonstrate the specifcity of ab231801 a Dot Blot analysis was performed using synthetic oligonucleotides containing different modifed C-bases (indicated in red).

125 and 25 ng of the respective oligo's were bound to a Streptavindin-coated multi-well plate. ab231801 was used at a dilution of 1 : 1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

IP, Dot, ICC/IF

applications

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Complementary Products

Primary Antibodies

AB106918

Anti-5-hydroxymethylcytosine (5-hmC) antibody [AB3/63.3] - ChIP Grade

ChIP - Anti-5-hydroxymethylcytosine (5-hmC) antibody [AB3/63.3] - ChIP Grade (AB106918)

  • 3
  • 2
Primary Antibodies

AB214728

Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)

  • 5
  • 1
Primary Antibodies

AB214727

Anti-5-methylcytosine (5-mC) antibody [RM231]

Recombinant

Immunocytochemistry - Anti-5-methylcytosine (5-mC) antibody [RM231] (AB214727)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Nucleic Acid": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>4 μg per IP.</p>", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/500 - 1/1000", "Dot-species-notes": "<p></p>" }, "Transfected cell line - Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

5-carboxylcytosine (5-caC) is an epigenetic modification of cytosine involved in DNA demethylation processes. Known also as 5-caC it represents an oxidative derivative of 5-methylcytosine. It usually appears in the genome of mammals. Researchers can detect 5-caC in embryonic stem cells and various tissues that undergo significant cellular differentiation. The exact molecular mass of 5-caC is not generally specified but as a part of a cytosine molecule it contributes to diverse molecular mass of DNA. Scientists use methods like mass spectrometry and antibody-based techniques to measure its presence.
Biological function summary

5-carboxylcytosine plays an essential role in gene regulation and development. It facilitates active DNA demethylation by serving as an intermediate in the TET (Ten-eleven translocation) protein-dependent pathway. Through its interactions it influences the methylation status of DNA impacting gene expression in important developmental stages. 5-caC does not necessarily form a complex independently but works along with TET proteins to remodel chromatin structure and affect transcriptional activity.

Pathways

5-carboxylcytosine functions prominently in the DNA methylation and demethylation pathway. These pathways are key to regulating gene expression and maintaining genomic stability. The TET enzymes convert 5-methylcytosine to 5-caC among other intermediates which then undergo further processing to unmodified cytosine. The involvement of proteins like TET1 TET2 and TET3 demonstrates the interconnected roles within these processes and their impacts on transcriptional regulation.

5-carboxylcytosine displays a significant relationship with cancer and neurological disorders. Aberrant patterns of 5-methylcytosine oxidation and disruption of the DNA demethylation pathway contribute to oncogenesis. The interaction with TET proteins links to several forms of leukemia and brain-related disorders such as Rett syndrome. Through these associations 5-caC serves as an important focus in studying disease mechanisms and potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information 5-carboxylcytosine (5-caC)
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 8:26 PubMed37236990

2023

Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Lynn Yap,Li Yen Chong,Clarissa Tan,Swarnaseetha Adusumalli,Millie Seow,Jing Guo,Zuhua Cai,Sze Jie Loo,Eric Lim,Ru San Tan,Elina Grishina,Poh Loong Soong,Narayan Lath,Lei Ye,Enrico Petretto,Karl Tryggvason
View all publications
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Product promise

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