Rabbit Polyclonal 5-HT-2B antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HTR2B aa 350 to C-terminus.
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Publications
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- The Journal of international medical research 48:3000605209707652020Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Liu,Deqi Xiong,Rizhao Pang,Qian Deng,Nianyi Sun,Jinqi Zheng,Jiancheng Liu,Wu Xiang,Zhesi Chen,Jiachun Lu,Wenchun Wang,Anren ZhangPubMed 33356694
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HTR2B aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P41595
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
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Target data
Function
G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:18703043, PubMed:23519210, PubMed:7926008, PubMed:8078486, PubMed:8143856, PubMed:8882600). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances (PubMed:12970106, PubMed:18703043, PubMed:23519210, PubMed:23519215, PubMed:24357322, PubMed:28129538, PubMed:30127358, PubMed:36087581, PubMed:7926008, PubMed:8078486, PubMed:8143856). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed:23519215, PubMed:28129538, PubMed:8078486, PubMed:8143856, PubMed:8882600). HTR2B is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (PubMed:18703043, PubMed:23519215, PubMed:28129538, PubMed:30127358, PubMed:36087581, PubMed:8078486, PubMed:8143856, PubMed:8882600). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed:23519215, PubMed:28129538, PubMed:30127358, PubMed:36087581). Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. May play a role in the perception of pain (By similarity). Plays a role in the regulation of behavior, including impulsive behavior (PubMed:21179162). Required for normal proliferation of embryonic cardiac myocytes and normal heart development (By similarity). Protects cardiomyocytes against apoptosis (By similarity). Plays a role in the adaptation of pulmonary arteries to chronic hypoxia (By similarity). Plays a role in vasoconstriction (By similarity). Required for normal osteoblast function and proliferation, and for maintaining normal bone density (By similarity). Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity).
Alternative names
5-hydroxytryptamine receptor 2B, 5-HT-2B, 5-HT2B, Serotonin receptor 2B, HTR2B
Recommended products
Rabbit Polyclonal 5-HT-2B antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HTR2B aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HTR2B aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P41595
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
5-HT-2B also known as serotonin receptor 2B is a G-protein-coupled receptor that binds to serotonin. This protein with a molecular mass of approximately 55 kDa is located on the cell surface and exhibits a wide expression in tissues such as the heart lung liver and gastrointestinal tract. It contains several functional domains contributing to its activity mediating cellular responses through signaling cascades when serotonin binds to it.
- Biological function summary
5-HT-2B plays a role in modulating the contractility and remodeling of cardiac tissue influencing vascular function and smooth muscle behavior impacting gastrointestinal motility and contributing to liver regeneration. It is not part of a large multiprotein complex but interacts with other cellular components to exert its biological functions. The receptor's activation leads to various intracellular responses influencing different physiological effects depending on the tissue context.
- Pathways
Serotonin receptor 2B is involved in the serotonin signaling pathway and the calcium signaling pathway. These pathways impact several physiological processes including mood regulation and cardiovascular function. The receptor interacts with proteins such as phospholipase C which mediates the hydrolysis of phospholipids in the membrane releasing inositol trisphosphate and causing a rise in intracellular calcium levels important for function continuation.
- Associated diseases and disorders
Abnormalities in 5-HT-2B have been linked to cardiac valvulopathy and pulmonary hypertension. These conditions highlight the importance of its role in vascular and cardiac function. The receptor's dysregulation interacts with proteins such as endothelin-1 in vascular abnormalities and contributes to disease progression highlighting its potential as a target for therapeutic interventions in these disorders.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-5-HT-2B antibody (ab221181)
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized SK-MEL-30 cells labeling 5-HT-2B with ab221181 at 4 μg/mL (green).
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