Goat Polyclonal 5-HT2C Receptor antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HTR2C aa 400-450.
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Publications
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- ACS chemical neuroscience 10:3241-32482019Endogenous Serotonin 5-HT and 5-HT Receptors Associate in the Medial Prefrontal Cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanda E Price et. al.PubMed 30645940
- CNS neuroscience & therapeutics 24:721-7332018Preferential modulation of the lateral habenula activity by serotonin-2A rather than -2C receptors: Electrophysiological and neuroanatomical evidence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancis Delicata et. al.PubMed 29479825
- The Journal of neuroscience : the official journal of the Society for Neuroscience 37:1378-139320165-HT Receptor Knockdown in the Amygdala Inhibits Neuropathic-Pain-Related Plasticity and Behaviors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangchen Ji,Wei Zhang,Lenin Mahimainathan,Madhusudhanan Narasimhan,Takaki Kiritoshi,Xiuzhen Fan,Jigong Wang,Thomas A Green,Volker NeugebauerPubMed 28011743
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HTR2C aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link P28335
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Chimpanzee | Predicted |
| Dog | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Chimpanzee | Dilution info - | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:12970106, PubMed:18703043, PubMed:19057895, PubMed:29398112, PubMed:7895773). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD) (PubMed:19057895, PubMed:29398112). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed:18703043, PubMed:29398112). HTR2C is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (PubMed:18703043, PubMed:29398112). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed:29398112). Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelanocortin neurons and the release of CRH that then regulates the release of corticosterone (By similarity). Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress (By similarity). Plays a role in insulin sensitivity and glucose homeostasis (By similarity).
Alternative names
HTR1C, HTR2C, 5-hydroxytryptamine receptor 2C, 5-HT-2C, 5-HT2C, 5-HTR2C, 5-hydroxytryptamine receptor 1C, Serotonin receptor 2C, 5-HT-1C, 5-HT1C
Recommended products
Goat Polyclonal 5-HT2C Receptor antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HTR2C aa 400-450.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HTR2C aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link P28335
- Purification technique
- Affinity purification Immunogen
- Specificity
Ab32887 recognises 5HT2C Receptor.
- Concentration
- Purification notes
Ab32887 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 5-HT2C receptor also known as 5-hydroxytryptamine receptor 2C is a G-protein coupled receptor in the serotonin receptor family. It has a mass of approximately 51 kDa. This receptor primarily expresses in the central nervous system including regions like the choroid plexus. Researchers note the 5-HT2C receptor's significant presence in areas responsible for regulating mood and cognition making it a critical player in neurotransmitter systems.
- Biological function summary
The 5-HT2C receptor plays a role in modulating neurotransmitter release. It is not part of a multi-subunit complex; instead it directly interacts with G proteins to exert its function. Upon activation by serotonin the receptor can influence the release of other neurotransmitters such as dopamine and norepinephrine. This interaction impacts various physiological processes including appetite control mood regulation and anxiety management.
- Pathways
One finds the 5-HT2C receptor involved in the serotonin signaling pathway contributing to mood and behavior regulation. The receptor links to the phosphatidylinositol signaling pathway where it engages proteins like phospholipase C. This connection is important for cellular responses to external stimuli. The 5-HT2C receptor also has relationships with other serotonin receptors like 5-HT2B which share similar signaling mechanisms within these pathways.
- Associated diseases and disorders
The 5-HT2C receptor has associations with conditions such as depression and obesity. Its regulatory role in neurotransmitter release influences mood disorders by affecting serotonin levels. Research has shown that the receptor connects with proteins like dopamine transporter which are relevant in the context of psychiatric disorders. In obesity altered receptor activity may affect appetite and energy balance contributing to weight regulation challenges.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-5-HT2C Receptor antibody (ab32887)
All lanes: Western blot - Anti-5-HT2C Receptor antibody (ab32887) at 1 µg/mL
All lanes: EBV immortalised Lymphoblastoid lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 1min
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