AB124951

Anti-5-HT2C Receptor antibody [EPR6486]

20ul selling size
RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal 5-HT2C Receptor antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HTR1C, HTR2C, 5-hydroxytryptamine receptor 2C, 5-HT-2C, 5-HT2C, 5-HTR2C, 5-hydroxytryptamine receptor 1C, Serotonin receptor 2C, 5-HT-1C, 5-HT1C

2 Images

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Western blot - Anti-5-HT2C Receptor antibody [EPR6486] (AB124951)

All lanes:

Western blot - Anti-5-HT2C Receptor antibody [EPR6486] (ab124951) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

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OI-RD Scanning - Anti-5-HT2C Receptor antibody [EPR6486] (AB124951)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-5-HT2C Receptor antibody [EPR6486] (AB124951)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-5-HT2C Receptor antibody [EPR6486] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6486

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5-HT2C receptor also known as 5-hydroxytryptamine receptor 2C is a G-protein coupled receptor in the serotonin receptor family. It has a mass of approximately 51 kDa. This receptor primarily expresses in the central nervous system including regions like the choroid plexus. Researchers note the 5-HT2C receptor's significant presence in areas responsible for regulating mood and cognition making it a critical player in neurotransmitter systems.

Biological function summary

The 5-HT2C receptor plays a role in modulating neurotransmitter release. It is not part of a multi-subunit complex; instead it directly interacts with G proteins to exert its function. Upon activation by serotonin the receptor can influence the release of other neurotransmitters such as dopamine and norepinephrine. This interaction impacts various physiological processes including appetite control mood regulation and anxiety management.

Pathways

One finds the 5-HT2C receptor involved in the serotonin signaling pathway contributing to mood and behavior regulation. The receptor links to the phosphatidylinositol signaling pathway where it engages proteins like phospholipase C. This connection is important for cellular responses to external stimuli. The 5-HT2C receptor also has relationships with other serotonin receptors like 5-HT2B which share similar signaling mechanisms within these pathways.

The 5-HT2C receptor has associations with conditions such as depression and obesity. Its regulatory role in neurotransmitter release influences mood disorders by affecting serotonin levels. Research has shown that the receptor connects with proteins like dopamine transporter which are relevant in the context of psychiatric disorders. In obesity altered receptor activity may affect appetite and energy balance contributing to weight regulation challenges.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 12970106, PubMed : 18703043, PubMed : 19057895, PubMed : 29398112, PubMed : 7895773). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD) (PubMed : 19057895, PubMed : 29398112). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 18703043, PubMed : 29398112). HTR2C is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (PubMed : 18703043, PubMed : 29398112). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed : 29398112). Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelanocortin neurons and the release of CRH that then regulates the release of corticosterone (By similarity). Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress (By similarity). Plays a role in insulin sensitivity and glucose homeostasis (By similarity).

See full target information HTR2C

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0287817 PubMed37788276

2023

Antioxioxidant and antiapoptotic effects of Thymosin β4 in Aβ-induced SH-SY5Y cells via the 5-HTR1A/ERK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Gui-Hong Zhang,Kai Ling Chin,Shi-Yan Yan,Rahmawati Pare

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-5-HT2C Receptor antibody [EPR6486]

Western blot - Anti-5-HT2C Receptor antibody [EPR6486] (AB124951)

OI-RD Scanning - Anti-5-HT2C Receptor antibody [EPR6486] (AB124951)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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