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AB248070

Anti-5-HT2C Receptor antibody [EPR6486] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal 5-HT2C Receptor antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HTR1C, HTR2C, 5-hydroxytryptamine receptor 2C, 5-HT-2C, 5-HT2C, 5-HTR2C, 5-hydroxytryptamine receptor 1C, Serotonin receptor 2C, 5-HT-1C, 5-HT1C

2 Images
Western blot - Anti-5-HT2C Receptor antibody [EPR6486] - BSA and Azide free (AB248070)
  • WB

Unknown

Western blot - Anti-5-HT2C Receptor antibody [EPR6486] - BSA and Azide free (AB248070)

This data was developed using ab124951, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-5-HT2C Receptor antibody [EPR6486] (<a href='/en-us/products/primary-antibodies/5-ht2c-receptor-antibody-epr6486-ab124951'>ab124951</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

false

OI-RD Scanning - Anti-5-HT2C Receptor antibody [EPR6486] - BSA and Azide free (AB248070)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-5-HT2C Receptor antibody [EPR6486] - BSA and Azide free (AB248070)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6486

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab248070 is the carrier-free version of ab124951.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 5-HT2C receptor also known as 5-hydroxytryptamine receptor 2C is a G-protein coupled receptor in the serotonin receptor family. It has a mass of approximately 51 kDa. This receptor primarily expresses in the central nervous system including regions like the choroid plexus. Researchers note the 5-HT2C receptor's significant presence in areas responsible for regulating mood and cognition making it a critical player in neurotransmitter systems.
Biological function summary

The 5-HT2C receptor plays a role in modulating neurotransmitter release. It is not part of a multi-subunit complex; instead it directly interacts with G proteins to exert its function. Upon activation by serotonin the receptor can influence the release of other neurotransmitters such as dopamine and norepinephrine. This interaction impacts various physiological processes including appetite control mood regulation and anxiety management.

Pathways

One finds the 5-HT2C receptor involved in the serotonin signaling pathway contributing to mood and behavior regulation. The receptor links to the phosphatidylinositol signaling pathway where it engages proteins like phospholipase C. This connection is important for cellular responses to external stimuli. The 5-HT2C receptor also has relationships with other serotonin receptors like 5-HT2B which share similar signaling mechanisms within these pathways.

The 5-HT2C receptor has associations with conditions such as depression and obesity. Its regulatory role in neurotransmitter release influences mood disorders by affecting serotonin levels. Research has shown that the receptor connects with proteins like dopamine transporter which are relevant in the context of psychiatric disorders. In obesity altered receptor activity may affect appetite and energy balance contributing to weight regulation challenges.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed : 12970106, PubMed : 18703043, PubMed : 19057895, PubMed : 29398112, PubMed : 7895773). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD) (PubMed : 19057895, PubMed : 29398112). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (PubMed : 18703043, PubMed : 29398112). HTR2C is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (PubMed : 18703043, PubMed : 29398112). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed : 29398112). Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelanocortin neurons and the release of CRH that then regulates the release of corticosterone (By similarity). Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress (By similarity). Plays a role in insulin sensitivity and glucose homeostasis (By similarity).
See full target information HTR2C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0190722 PubMed36946735

2023

Characterization of the Impact of Merkel Cell Polyomavirus-Induced Interferon Signaling on Viral Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Wang,June F Yang,Taylor E Senay,Wei Liu,Jianxin You
View all publications
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