Rabbit Recombinant Monoclonal 5 Lipoxygenase/5-LO antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human samples. Cited in 41 publications.
Images
![Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (AB169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--western-blot-img130785.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (AB169755)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (AB169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118624.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (AB169755)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (AB169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80009.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (AB169755)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (AB169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img63011.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (AB169755)")
![Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (AB169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--flow-cytometry-intracellular-img37354.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (AB169755)")
Publications
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- BMC gastroenterology 23:4152023Association between high expression of intratumoral fibroblast activation protein and survival in patients with intrahepatic cholangiocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhei Waki,Yuji Morine,Takayuki Noma,Chie Takasu,Hiroki Teraoku,Shinichiro Yamada,Yu Saito,Tetsuya Ikemoto,Mitsuo ShimadaPubMed 38017374
- Frontiers in immunology 14:13242052023Lipid metabolism-related gene expression in the immune microenvironment predicts prognostic outcomes in renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Zhang,Bingbiao Lin,Huikun Chen,Yinyan Ye,Yijie Huang,Zhen Chen,Jun LiPubMed 38090559
- International journal of molecular sciences 24:2023Effect of L. on the Metabolism of Arachidonic Acid in the Isolated Kidney of a Rat Model of Metabolic Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsrael Pérez-Torres,María Elena Soto,Linaloe Manzano-Pech,Eulises Díaz-Díaz,Raúl Martínez-Memije,Juan Carlos Torres-Narváez,Verónica Guarner-Lans,Vicente Castrejón-TéllezPubMed 37762512
- International journal of oncology 63:2023Human cytomegalovirus infection enhances 5‑lipoxygenase and cycloxygenase‑2 expression in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMattia Russel Pantalone,Nerea Martin Almazan,Rossano Lattanzio,Chato Taher,Simone De Fabritiis,Silvia Valentinuzzi,Faraz Bishehsari,Mahboobeh Mahdavinia,Fabio Verginelli,Afsar Rahbar,Renato Mariani-Costantini,Cecilia Söderberg-NauclerPubMed 37654195
- Scientific reports 13:132392023Inhibition of CysLTR1 reduces the levels of aggregated proteins in retinal pigment epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndreas Koller,Susanne Maria Brunner,Julia Preishuber-Pflügl,Daniela Mayr,Anja-Maria Ladek,Christian Runge,Herbert Anton Reitsamer,Andrea TrostPubMed 37580467
- Methods in molecular biology (Clifton, N.J.) 2712:211-2222023Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Zhong,Ruochan ChenPubMed 37578709
- Nanotoxicology 17:249-2692023Multi-walled carbon nanotubes induce arachidonate 5-lipoxygenase expression and enhance the polarization and function of M1 macrophages .Applications:Unspecified applicationReactive species:Unspecified reactive speciesChol Seung Lim,Brandon Veltri,Michael Kashon,Dale W Porter,Qiang MaPubMed 37115655
- International journal of inflammation 2023:54801992023N-Acetylcysteine Decreases Myocardial Content of Inflammatory Mediators Preventing the Development of Inflammation State and Oxidative Stress in Rats Subjected to a High-Fat Diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKlaudia Sztolsztener,Wiktor Bzdęga,Katarzyna Hodun,Adrian ChabowskiPubMed 36941865
- Biomolecules 13:2023The Placentas of Women Who Suffer an Episode of Psychosis during Pregnancy Have Increased Lipid Peroxidation with Evidence of Ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiguel A Ortega,Oscar Fraile-Martinez,Cielo García-Montero,Rosa M Funes Moñux,Sonia Rodriguez-Martín,Coral Bravo,Juan A De Leon-Luis,Jose V Saz,Miguel A Saez,Luis G Guijarro,Guillermo Lahera,Fernando Mora,Sonia Fernandez-Rojo,Javier Quintero,Jorge Monserrat,Natalio García-Honduvilla,Julia Bujan,Melchor Alvarez-Mon,Miguel Angel Alvarez-MonPubMed 36671505
- Cancer gene therapy 30:108-1232022Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHannah Weisser,Tamara Göbel,G Melissa Krishnathas,Marius Kreiß,Carlo Angioni,Duran Sürün,Dominique Thomas,Tobias Schmid,Ann-Kathrin Häfner,Astrid S KahntPubMed 36114329
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Expected | Expected | Expected |
| Dog | Predicted | Not recommended | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation (PubMed:19022417, PubMed:21233389, PubMed:22516296, PubMed:23246375, PubMed:24282679, PubMed:24893149, PubMed:31664810, PubMed:8615788, PubMed:8631361). Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE) (PubMed:23246375). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene (PubMed:31664810). Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses (PubMed:17114001, PubMed:21206090, PubMed:31664810, PubMed:32404334, PubMed:8615788). Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma) (By similarity). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers (PubMed:31664810). In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes (By similarity). Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40 (PubMed:21200133). May also play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK (By similarity). Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells (By similarity).
Alternative names
LOG5, ALOX5, Polyunsaturated fatty acid 5-lipoxygenase, Arachidonate 5-lipoxygenase, 5-LO, 5-lipoxygenase
Recommended products
Rabbit Recombinant Monoclonal 5 Lipoxygenase/5-LO antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human samples. Cited in 41 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP6072(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
5-Lipoxygenase also known as 5-LO or 5-LOX is an enzyme that catalyzes the oxidation of arachidonic acid to produce leukotrienes which are signaling molecules in the body. 5-LOX has a molecular weight of approximately 78 kDa. It is mainly expressed in leukocytes like macrophages neutrophils and mast cells. This enzyme plays an important role in the inflammatory response and the regulation of immune functions in these cells.
- Biological function summary
The activity of 5-Lipoxygenase contributes to the production of leukotrienes which are pivotal in mediating inflammatory and allergic responses. This enzyme operates alone but it often interacts with 5-LOX-activating protein (FLAP) to facilitate the synthesis of leukotrienes efficiently. These interactions suggest that 5-LOX has a significant role in cellular processes related to inflammation and immune reactions.
- Pathways
5-Lipoxygenase is integral to the arachidonic acid metabolic pathway initiating the biosynthesis of leukotrienes from arachidonic acid. This pathway also involves other enzymes like phospholipase A2 which releases arachidonic acid from membrane phospholipids and cyclooxygenase enzymes (COX) which convert arachidonic acid into prostaglandins. The end products of this pathway including leukotrienes manage and resolve inflammation and immune responses illustrating the collaborative effort between these enzymes.
- Associated diseases and disorders
5-Lipoxygenase is significantly linked to inflammatory diseases such as asthma and atherosclerosis. In asthma the overproduction of leukotrienes due to enhanced 5-LOX activity results in bronchoconstriction and inflammation of the airways. In atherosclerosis leukotrienes contribute to the development and progression of plaques driving chronic inflammation in the vascular system. Additionally through its pathway its activity relates closely to cyclooxygenase enzymes showing its importance in disease mechanisms targeted by anti-inflammatory therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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9 product images
![Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--western-blot-img130785.jpg "Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution
Lane 1: Rat heart lysate at 20 µg
Lane 2: Rat kidney lysate at 20 µg
Lane 3: Rat spleen lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 78 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118624.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
ab169755 staining 5 Lipoxygenase/5-LO in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80009.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img63011.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--flow-cytometry-intracellular-img37354.jpg "Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
Flow Cytometry (Intracellular) - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
ab169755 staining 5 Lipoxygenase/5-LO in HUVEC (human umbilical vein/vascular endothelium) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/130. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (black).
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (blue).
![Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--western-blot-img94708.jpg "Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution
All lanes: K562 cell Lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 78 kDa
![Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--western-blot-img95564.jpg "Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution
All lanes: Human fetal thymus at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 78 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--immunocytochemistry-immunofluorescence-img84711.jpg "Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
Immunofluorescent analysis of HUVEC cells labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.
![Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--western-blot-img106795.jpg "Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)
All lanes: Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755) at 1/1000 dilution
Lane 1: Fetal thymus lysate at 10 µg
Lane 2: K562 lysate at 10 µg
Lane 3: HUVEC lysate at 10 µg
Predicted band size: 78 kDa
![Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--western-blot-img94708.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
![Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--western-blot-img95564.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
![Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--immunocytochemistry-immunofluorescence-img84711.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
![Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755), expandable thumbnail](https://content.abcam.com/products/images/5-lipoxygenase-5-lo-antibody-ep60722-ab169755--western-blot-img106795.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-5 Lipoxygenase/5-LO antibody [EP6072(2)] (ab169755)")
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