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AB10805

Anti-5-methylcytosine (5-mC) antibody [33D3]

5

(12 Reviews)

|

(228 Publications)

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) is a mouse monoclonal antibody detecting 5-methylcytosine (5-mC) in Flow Cytometry, IP, IHC-P, IHC-Fr.

- Over 170 publications
- Trusted since 2005

View Alternative Names

5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)
  • IHC-P

AbReview13478****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.

This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

33D3

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Applications

IHC-Fr, IP, Flow Cyt, SB, IHC-P

applications

Specificity

Modified base 5-methylcytidine found in DNA of plants and vertebrates.

Raised against the modified ribonucleoside. Specific for the presence of a methyl group on carbon 5 of the pyrimidine ring.

Reactivity data

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Product details

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) is a mouse monoclonal antibody and is validated for use in Flow Cyt, IHC-Fr, IHC-P, IP, SB in human samples.

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has been cited over 177 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has high sensitivity and specificity.

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has 11 independent reviews from customers.

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) specifically detects 5-methylcytosine (5-mC) (UniProt ID: ; Molecular weight: kDa) and is sold in 50 µg selling sizes.

Storage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS, 0.876% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

5-methylcytosine often referred to as 5-mC 5-methyl cytosine or 5-methylcytidine is a methylated form of the nucleobase cytosine found in the DNA of various organisms. This modification typically occurs at the carbon 5 position of the cytosine ring contributing to the epigenetic regulation of gene expression. While the mass of 5-mC itself is relatively small since it is a modified nucleotide its impact on gene regulation is significant. 5-mC is widely expressed in the genome of higher eukaryotes particularly in CpG dinucleotides where it plays a role in mammalian development and cell differentiation.
Biological function summary

Cytosine methylation affects DNA-protein interactions leading to changes in chromatin structure and the regulation of gene activity. 5-methylcytosine is an important player in the chromatin modification complex where it collaborates with other epigenetic marks to control gene expression profiles. This modified nucleotide helps to stabilize gene silencing maintaining the closed chromatin state which inhibits the transcription machinery's access to specific genomic regions. Such silencing is vital during processes like X-chromosome inactivation and imprinting.

Pathways

Researchers have identified 5-mC within two critical pathways: DNA methylation and demethylation cycle and histone modification pathway. In the DNA methylation pathway 5-mC interacts closely with proteins like DNA methyltransferases (DNMTs) which directly add methyl groups to cytosine bases. In the context of histone modification 5-mC influences the binding of methyl-CpG-binding domain (MBD) proteins which read and interpret methylated DNA marks affecting histone modification and DNA accessibility.

The dysregulation of 5-methylcytosine patterns associates prominently with cancer and neurological disorders. Abnormal methylation patterns where 5-mC is either hypermethylated or hypomethylated can lead to the silencing of tumor suppressor genes or activation of oncogenes forming a basis for cancer development. In neurological disorders altered 5-mC levels link to conditions such as Rett syndrome where the dysfunction of the associated protein MeCP2 an important reader of 5-mC contributes to the disease pathology.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (228)

Recent publications for all applications. Explore the full list and refine your search

Clinical epigenetics 17:151 PubMed40993735

2025

ALYREF inhibits ferroptosis in vascular endothelial cells and improves atherosclerosis by epigenetic modification of CISD1.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghai Hua,Ling Yu,Wenjun Xiong,Ru Ying,Qiong Duan,Jianbing Zhu,Xiaoping Peng,Minzi Qiu

Biological & pharmaceutical bulletin 48:1343-1350 PubMed40930835

2025

Kaempferol Inhibits Ferroptosis in Lung Epithelial Cells in LPS-Induced Acute Lung Injury via m5C Methylation of TFRC.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Weihua Wu,Peng An,Zhenfei Yu

Oncogene 44:3609-3624 PubMed40764753

2025

MUC1-C auto-regulatory complex with EBNA1 is responsible for latent Epstein-Barr virus-associated gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Ozawa,Yin Wang,Henry G Withers,Naoki Haratake,Ayako Nakashoji,Atrayee Bhattacharya,Atsushi Fushimi,Chie Kikutake,Kazuhiro Yamanoi,Shaowen White,Keyi Wang,Tatsuaki Daimon,Keisuke Shigeta,Kazumasa Fukuda,Hirofumi Kawakubo,Yuko Kitagawa,Mark D Long,Benjamin E Gewurz,Donald Kufe

Frontiers in molecular neuroscience 18:1628860 PubMed40747242

2025

Tet3-mediated DNA demethylation is essential for maintaining the dedifferentiation capacity of mammalian Müller glia.

Applications

Unspecified application

Species

Unspecified reactive species

Erick J Martinez-Colin,Ivonne Lezama,Lenin D Ochoa-de la Paz,Monica Lamas

Journal of ovarian research 18:162 PubMed40707993

2025

5-methylcytosine regulated CCNL2 promotes tumorigenesis and cisplatin resistance of ovarian cancer with therapeutic implications.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Guiyun Cheng,Wenwen Jiang,Beihua Kong,Shu Yao,Xihan Liu

Molecular cancer 24:201 PubMed40691844

2025

NSUN2-mediated cytosine-5 methylation of FSP1 protects acute myeloid leukemia cells from ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenle Ye,Yanchun Zhao,Yutong Zhou,Jiansong Huang,Xiao He,Zhixin Ma,Xin Huang,Chao Hu,Fenglin Li,Qing Ling,Huafeng Wang,Hongyan Tong,Jie Sun,Jie Jin

Animal bioscience 38:1973-1983 PubMed40241588

2025

Parental betaine supplementation promotes hepatic conversion of cholesterol to bile acids in offspring goslings with epigenetic modulation of CYP7A1 gene.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Ma,Liang Chen,Yan Wang,Lei Wu,Ruqian Zhao

Journal of experimental & clinical cancer research : CR 44:121 PubMed40234937

2025

LINC02167 stabilizes KSR1 mRNA in an mC-dependent manner to regulate the ERK/MAPK signaling pathway and promotes colorectal cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Junwen Qi,Tao Jiang,Bowen Liu,Qihang Hu,Junnan Chen,Ning Ma,Yixin Xu,Hu Song,Jun Song

Cancer communications (London, England) 45:846-879 PubMed40227950

2025

Novel molecular mechanisms of immune evasion in hepatocellular carcinoma: NSUN2-mediated increase of SOAT2 RNA methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Jiang,Feng Liu,Dan Cui,Caixia Xu,Jiachang Chi,Tinghua Yan,Fang Guo

Journal of translational medicine 23:385 PubMed40170133

2025

CircPRKCA facilitates esophageal squamous cell carcinoma metastasis via m5C-dependent CSF2 mRNA stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Wu,Lina Gu,Yang Zheng,Jingjing Liu,Zishuan Wei,Fei Liu,Jiali Li,Lingjiao Meng,Yang Sang,Meixiang Sang,Lianmei Zhao,Baoen Shan
View all publications

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