Anti-5-methylcytosine (5-mC) antibody [33D3]
5
(12 Reviews)
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(228 Publications)
Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) is a mouse monoclonal antibody detecting 5-methylcytosine (5-mC) in Flow Cytometry, IP, IHC-P, IHC-Fr.
- Over 170 publications
- Trusted since 2005
View Alternative Names
5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG
- IHC-P
AbReview13478****
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)
ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.
This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig
Reactivity data
Product details
Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has been cited over 177 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has high sensitivity and specificity.
Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has 11 independent reviews from customers.
Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) specifically detects 5-methylcytosine (5-mC) (UniProt ID: ; Molecular weight: kDa) and is sold in 50 µg selling sizes.
Storage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.
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Supplementary information
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Biological function summary
Cytosine methylation affects DNA-protein interactions leading to changes in chromatin structure and the regulation of gene activity. 5-methylcytosine is an important player in the chromatin modification complex where it collaborates with other epigenetic marks to control gene expression profiles. This modified nucleotide helps to stabilize gene silencing maintaining the closed chromatin state which inhibits the transcription machinery's access to specific genomic regions. Such silencing is vital during processes like X-chromosome inactivation and imprinting.
Pathways
Researchers have identified 5-mC within two critical pathways: DNA methylation and demethylation cycle and histone modification pathway. In the DNA methylation pathway 5-mC interacts closely with proteins like DNA methyltransferases (DNMTs) which directly add methyl groups to cytosine bases. In the context of histone modification 5-mC influences the binding of methyl-CpG-binding domain (MBD) proteins which read and interpret methylated DNA marks affecting histone modification and DNA accessibility.
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Target data
Publications (228)
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Clinical epigenetics 17:151 PubMed40993735
2025
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Biological & pharmaceutical bulletin 48:1343-1350 PubMed40930835
2025
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Oncogene 44:3609-3624 PubMed40764753
2025
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Frontiers in molecular neuroscience 18:1628860 PubMed40747242
2025
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Journal of ovarian research 18:162 PubMed40707993
2025
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Molecular cancer 24:201 PubMed40691844
2025
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Animal bioscience 38:1973-1983 PubMed40241588
2025
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Journal of experimental & clinical cancer research : CR 44:121 PubMed40234937
2025
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Cancer communications (London, England) 45:846-879 PubMed40227950
2025
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Journal of translational medicine 23:385 PubMed40170133
2025
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com