Mouse Monoclonal 5-Methylcytosine antibody. Suitable for SB, ICC/IF and reacts with Chemical samples. Cited in 33 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to 5-Methylcytosine.
IgM
Mouse
pH: 6 - 8.5
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
SB | ICC/IF | |
---|---|---|
Chemical | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info 1/1000 | Notes Immunoblotting - detection of DNA with 5-methylocytosine on nitrocellulose. Use at 1/1000 |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info 1/50.00000 - 1/100.00000 | Notes Recommend fixing in 4% PFA followed by denaturation with 70% formamide in 2x SSC and chilling in 70%, 95%, and 99% EtOH for 2 min each. Diluting antibody in SSC (saline sodium citrate) may give optimal results. See protocols from PubMed: 11983892 and 17994007 |
Select an associated product type
5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG
Mouse Monoclonal 5-Methylcytosine antibody. Suitable for SB, ICC/IF and reacts with Chemical samples. Cited in 33 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to 5-Methylcytosine.
5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG
IgM
Mouse
pH: 6 - 8.5
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
5MC-CD
ab73938 reacts with DNA with 5-Methylcytosine (methylated DNA).
ab73938 is purified and filter-sterilised.
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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5-methylcytosine often referred to as 5-mC 5-methyl cytosine or 5-methylcytidine is a methylated form of the nucleobase cytosine found in the DNA of various organisms. This modification typically occurs at the carbon 5 position of the cytosine ring contributing to the epigenetic regulation of gene expression. While the mass of 5-mC itself is relatively small since it is a modified nucleotide its impact on gene regulation is significant. 5-mC is widely expressed in the genome of higher eukaryotes particularly in CpG dinucleotides where it plays a role in mammalian development and cell differentiation.
Cytosine methylation affects DNA-protein interactions leading to changes in chromatin structure and the regulation of gene activity. 5-methylcytosine is an important player in the chromatin modification complex where it collaborates with other epigenetic marks to control gene expression profiles. This modified nucleotide helps to stabilize gene silencing maintaining the closed chromatin state which inhibits the transcription machinery's access to specific genomic regions. Such silencing is vital during processes like X-chromosome inactivation and imprinting.
Researchers have identified 5-mC within two critical pathways: DNA methylation and demethylation cycle and histone modification pathway. In the DNA methylation pathway 5-mC interacts closely with proteins like DNA methyltransferases (DNMTs) which directly add methyl groups to cytosine bases. In the context of histone modification 5-mC influences the binding of methyl-CpG-binding domain (MBD) proteins which read and interpret methylated DNA marks affecting histone modification and DNA accessibility.
The dysregulation of 5-methylcytosine patterns associates prominently with cancer and neurological disorders. Abnormal methylation patterns where 5-mC is either hypermethylated or hypomethylated can lead to the silencing of tumor suppressor genes or activation of oncogenes forming a basis for cancer development. In neurological disorders altered 5-mC levels link to conditions such as Rett syndrome where the dysfunction of the associated protein MeCP2 an important reader of 5-mC contributes to the disease pathology.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamidomonas me-1 cells. Left: DAPI-stained cells. Middle: Cells stained with ab73938 and FITC-conjugated 2nd antibody. Right: Merged image. Chloroplast DNA is exclusively methylated in gamete cells
ab73938 staining 5-Methyl Cytosine in murine PN5 zygotes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with methanol/acetic acid (3:1) and blocked with 30% serum for overnight a 4°C. Samples were incubated with primary antibody (1/100 in diluent) for 18 hours at 4°C before detection with a fluorescent secondary antibody.
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