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AB225681

Anti-50S ribosomal protein L7/L12 antibody

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(2 Publications)

Rabbit Polyclonal RPL7 antibody. Suitable for WB and reacts with Recombinant full length protein - Staphylococcus aureus samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Staphylococcus aureus subsp. aureus Mu50 rplL.

View Alternative Names

SAV0540, rplL, Large ribosomal subunit protein bL12, 50S ribosomal protein L7/L12, rplL

1 Images
Western blot - Anti-50S ribosomal protein L7/L12 antibody (AB225681)
  • WB

Supplier Data

Western blot - Anti-50S ribosomal protein L7/L12 antibody (AB225681)

Predicted MWt including 6xHis-SUMO tag : 29 kDa

All lanes:

Western blot - Anti-50S ribosomal protein L7/L12 antibody (ab225681) at 1/500 dilution

Lane 1:

Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.08 µg

Lane 2:

Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.04 µg

Lane 3:

Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 protein with 6xHis-SUMO tag at the N-terminal at 0.025 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 13 kDa

Observed band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Staphylococcus aureus

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Staphylococcus aureus subsp. aureus Mu50 rplL.

P66061

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Complementary Products

Primary Antibodies

AB190467

Anti-alpha-hemolysin antibody [8B7] - N-terminal

Western blot - Anti-alpha-hemolysin antibody [8B7] - N-terminal (AB190467)

  • 0
  • 0
Primary Antibodies

AB190465

Anti-entB antibody [3D10]

Functional Studies (Neut/Block) - Anti-entB antibody [3D10] (AB190465)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB225679

Anti-Exfoliative Toxin A/ETA antibody

Western blot - Anti-Exfoliative Toxin A/ETA antibody (AB225679)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Staphylococcus aureus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" }, "Staphylococcus aureus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

50S ribosomal protein L7/L12 is an important component of the 50S subunit of the ribosome known for its essential role in protein synthesis. It is also referred to as RPL7/L12. The protein is necessary for the binding of translation factors and is involved in the translation process. This protein has a molecular mass of approximately 12 kDa and expresses mainly in prokaryotic organisms. It contributes to ribosomal structure and function allowing the ribosome to carry out its role in translating mRNA into proteins.
Biological function summary

This protein belongs to the ribosomal L7/L12 family and functions as a part of the ribosomal protein complex. It forms a homodimer and several L7/L12 dimers together create a helical structure that is essential for the ribosomal function. The protein facilitates interactions with elongation factors such as EF-G and EF-Tu during protein synthesis. By forming part of the ribosomal machinery it supports the accuracy and efficiency of translating genetic information into functional proteins.

Pathways

The 50S ribosomal protein L7/L12 participates actively in the translation pathway important for cellular protein synthesis. It directly interacts with other ribosomal proteins and elongation factors making itself integral in the translation machinery's operation. The protein impacts the formation of the elongation complex connecting to translation-related pathways and closely interacting with other ribosomal subunits like 30S ribosomal proteins.

Abnormalities involving this protein affect bacterial resistance and potentially the effectiveness of antibiotics such as tetracyclines and macrolides. 50S ribosomal protein L7/L12's role in translation makes it a target in developing antibiotics particularly those that inhibit protein synthesis. This connection associates it with diseases related to bacterial infections where the effectiveness of the drug can hinge on precise interactions between the drug and the ribosomal protein. Additionally protein L7/L12 is implicated in antibiotic resistance linking it to disorders such as multidrug-resistant bacterial infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.
See full target information rplL

Additional targets

50S ribosomal protein L7/L12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 603:5529-5545 PubMed40946331

2025

Clenbuterol induces lean mass and muscle protein accretion, but attenuates cardiorespiratory fitness and desensitizes muscle β-adrenergic signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Morten Hostrup,Lukas Moesgaard,Mads Fischer,Kate Aiko Wickham,Mads Pleshardt,Andreas Breenfeldt Andersen,Jacob Bejder,Martin Thomassen,Jens J Nielsen,Yvette Dehnes,Jens Bangsbo,Nikolai B Nordsborg,Søren Jessen

Nature communications 16:7231 PubMed40769975

2025

Antimicrobial peptide class that forms discrete β-barrel stable pores anchored by transmembrane helices.

Applications

Unspecified application

Species

Unspecified reactive species

Seth W Dickey,Dylan J Burgin,Ama N Antwi,Amer Villaruz,Madeline R Galac,Gordon Y C Cheung,Tatiana K Rostovtseva,Liam J Worrall,Aleksander C Lazarski,Elio A Cino,D Peter Tieleman,Sergey M Bezrukov,Natalie C J Strynadka,Michael Otto
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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