Rabbit Polyclonal 53BP1 antibody. Suitable for WB, IP, Flow Cyt, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human TP53BP1 aa 1400-1700.
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- Nature methods 20:706-7132023Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq.Applications:IFReactive species:HumanRoger S Zou,Yang Liu,Oscar E Reyes Gaido,Maximilian F Konig,Brian J Mog,Leo L Shen,Franklin Aviles-Vazquez,Alberto Marin-Gonzalez,Taekjip HaPubMed 37024653
- Chemico-biological interactions 370:1103302022Synergistic effect of naphthalenediimide and squaraine ligand targeting G-quadruplex DNA in cancer cells.Applications:IFReactive species:HumanXueyu Hao,Chunjie Li,Hongyu Zhao,Wanli Wei,Chengbin Li,Rizhe Jin,Chuanqing Kang,Lianxun GaoPubMed 36563735
- Science translational medicine 14:eabj43752022Senolytic treatment preserves biliary regenerative capacity lost through cellular senescence during cold storage.Applications:IHCReactive species:MouseSofia Ferreira-Gonzalez,Tak Yung Man,Hannah Esser,Rhona Aird,Alastair M Kilpatrick,Daniel Rodrigo-Torres,Nicholas Younger,Lara Campana,Victoria L Gadd,Benjamin Dwyer,Niya Aleksieva,Luke Boulter,Mark T Macmillan,Yinmiao Wang,Katie J Mylonas,David A Ferenbach,Timothy J Kendall,Wei-Yu Lu,Juan Carlos Acosta,Dominic Kurian,Stephen O'Neill,Gabriel C Oniscu,Jesus M Banales,Paul J Krimpenfort,Stuart J ForbesPubMed 36475903
- Oxidative medicine and cellular longevity 2022:79572552022Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Feng Wei,Ching-Hsueh Cheng,Shu-Yu Wen,Jui-Chueh Lin,Yu-Hsuan Chen,Chun-Wei Wang,Yi-Hsuan Lee,Sung-Hsin KuoPubMed 36092168
- Cells 11:2022USP29 Deubiquitinates SETD8 and Regulates DNA Damage-Induced H4K20 Monomethylation and 53BP1 Focus Formation.Applications:WB, IFReactive species:HumanYeray Hernández-Reyes,María Cristina Paz-Cabrera,Raimundo Freire,Veronique A J SmitsPubMed 36010569
- Cell research 32:254-2682022Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaochen Gai,Di Xin,Duo Wu,Xin Wang,Linlin Chen,Yiqing Wang,Kai Ma,Qilin Li,Peng Li,Xiaochun YuPubMed 34980897
- Biomedicines 9:2021Association of Caspase 3 Activation and H2AX γ Phosphorylation in the Aging Brain: Studies on Untreated and Irradiated Mice.Applications:IFReactive species:MouseNadia Gionchiglia,Alberto Granato,Adalberto Merighi,Laura LossiPubMed 34572352
- Frontiers in pharmacology 11:6172702021USP7 Inhibition Alleviates HO-Induced Injury in Chondrocytes via Inhibiting NOX4/NLRP3 Pathway.Applications:WBReactive species:RatGang Liu,Qingbai Liu,Bin Yan,Ziqiang Zhu,Yaozeng XuPubMed 33584299
- Oncology reports 44:2678-26902020miR‑7/SP1/TP53BP1 axis may play a pivotal role in NSCLC radiosensitivity.
- Nature communications 11:23762020Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective properties.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: Water
- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human TP53BP1 aa 1400-1700. The exact immunogen used to generate this antibody is proprietary information. Database link Q12888
Consider this alternative
Reactivity data
Select an application| WB | IP | Flow Cyt | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected | Expected |
| Rat | Tested | Expected | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).
Alternative names
TP53-binding protein 1, 53BP1, p53-binding protein 1, p53BP1, TP53BP1
Recommended products
Rabbit Polyclonal 53BP1 antibody. Suitable for WB, IP, Flow Cyt, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human TP53BP1 aa 1400-1700.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: Water
- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human TP53BP1 aa 1400-1700. The exact immunogen used to generate this antibody is proprietary information. Database link Q12888
- Purity
Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Flow Cytometry - Anti-53BP1 antibody (ab172580)
Flow Cytometry analysis of HeLa cells labelling 53BP1 with ab172580 at 1:800. Cells were fixed with 1% PFA. Cells were incuabted with the primary antibody overnight at 4°C.
Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody (ab172580)
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling 53BP1 with ab172580 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Top - 53BP1, Middle - DAPI, Bottom - Merge.
Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody (ab172580)
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling 53BP1 with ab172580 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature.
Immunoprecipitation - Anti-53BP1 antibody (ab172580)
Immunoprecipitation analysis of U2OS cells labelling 53BP1 with ab172580. Protein A beads were crosslinked to the serum.
All lanes: Immunoprecipitation - Anti-53BP1 antibody (ab172580)
Predicted band size: 214 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab172580)
Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling 53BP1 with ab172580 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9.
Western blot - Anti-53BP1 antibody (ab172580)
All lanes: Western blot - Anti-53BP1 antibody (ab172580)
Lane 1: U2OS (Control si)
Lane 2: U2OS (53BP1 si)
Lane 3: NIH 3T3 cell lysate at 40 µg
Lane 4: PC12 cell lysate at 40 µg
Predicted band size: 214 kDa
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