Rabbit Polyclonal 53BP1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 104 publications. Immunogen corresponding to Synthetic Peptide within Human TP53BP1.
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- Biochemistry and biophysics reports 35:1014992023Wnt3a signaling with serum supply induces replication stress in cultured cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Wang,Rui Wang,Haiying Ma,Mengsu Yang,Zigang Li,Liang ZhangPubMed 37601449
- Cell reports 42:1125382023Multi-omics analysis reveals distinct non-reversion mechanisms of PARPi resistance in BRCA1- versus BRCA2-deficient mammary tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhyuk Bhin,Mariana Paes Dias,Ewa Gogola,Frank Rolfs,Sander R Piersma,Roebi de Bruijn,Julian R de Ruiter,Bram van den Broek,Alexandra A Duarte,Wendy Sol,Ingrid van der Heijden,Christina Andronikou,Taina S Kaiponen,Lara Bakker,Cor Lieftink,Ben Morris,Roderick L Beijersbergen,Marieke van de Ven,Connie R Jimenez,Lodewyk F A Wessels,Sven Rottenberg,Jos JonkersPubMed 37209095
- iScience 25:1051152022RPRM negatively regulates ATM levels through its nuclear translocation on irradiation mediated by CDK4/6 and IPO11.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYarui Zhang,Guomin Ou,Zhujing Ye,Zhou Zhou,Qianlin Cao,Mengting Li,Jingdong Wang,Jianping Cao,Hongying YangPubMed 36185355
- Nature communications 13:42852022DNA nicks induce mutational signatures associated with BRCA1 deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Li Feng,Qian Liu,Ruo-Dan Chen,Si-Cheng Liu,Zhi-Cheng Huang,Kun-Ming Liu,Xiao-Ying Yang,An-Yong XiePubMed 35879372
- NAR cancer 4:zcac0232022KMT2C-deficient tumors have elevated APOBEC mutagenesis and genomic instability in multiple cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoju Hu,Antara Biswas,Subhajyoti DePubMed 35898555
- Cold Spring Harbor molecular case studies :2022PHIP variants associated with Chung-Jansen syndrome disrupt replication fork stability and genome integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeysha Tirado-Class,Caitlin Hathaway,Wendy K Chung,Huzefa DungrawalaPubMed 35863899
- Molecular cell 81:4692-4708.e92021Loss of nuclear DNA ligase III reverts PARP inhibitor resistance in BRCA1/53BP1 double-deficient cells by exposing ssDNA gaps.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Paes Dias,Vivek Tripathi,Ingrid van der Heijden,Ke Cong,Eleni-Maria Manolika,Jinhyuk Bhin,Ewa Gogola,Panagiotis Galanos,Stefano Annunziato,Cor Lieftink,Miguel Andújar-Sánchez,Sanjiban Chakrabarty,Graeme C M Smith,Marieke van de Ven,Roderick L Beijersbergen,Jirina Bartkova,Sven Rottenberg,Sharon Cantor,Jiri Bartek,Arnab Ray Chaudhuri,Jos JonkersPubMed 34555355
- International journal of molecular sciences 22:2021G-Quadruplex Structures Colocalize with Transcription Factories and Nuclear Speckles Surrounded by Acetylated and Dimethylated Histones H3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenisa Komůrková,Alena Svobodová Kovaříková,Eva BártováPubMed 33671470
- mBio 12:2021Herpes Simplex Virus 1 Manipulates Host Cell Antiviral and Proviral DNA Damage Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMax E Mertens,David M KnipePubMed 33563816
- Cell reports 34:1086692021DCAF14 promotes stalled fork stability to maintain genome integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArik Townsend,Gabriella Lora,Justin Engel,Neysha Tirado-Class,Huzefa DungrawalaPubMed 33503431
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TP53BP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q12888
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/4000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
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Target data
Function
Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:17190600, PubMed:21144835, PubMed:22553214, PubMed:23333306, PubMed:27153538, PubMed:28241136, PubMed:31135337, PubMed:37696958). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23333306, PubMed:23727112, PubMed:27153538, PubMed:31135337). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:17190600, PubMed:23760478, PubMed:27153538, PubMed:28241136). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).
Alternative names
TP53-binding protein 1, 53BP1, p53-binding protein 1, p53BP1, TP53BP1
Recommended products
Rabbit Polyclonal 53BP1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 104 publications. Immunogen corresponding to Synthetic Peptide within Human TP53BP1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TP53BP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q12888
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
53BP1 also known as p53-binding protein 1 is a protein that plays an important role in DNA damage response. The 53BP1 protein has a molecular weight of approximately 213 kDa. It is a nuclear protein and it mainly expresses in cells during the DNA damage repair processes. Researchers commonly utilize immunofluorescence and anti-53BP1 antibodies to detect its presence especially for studying DNA repair mechanisms. 53BP1 proves important for maintenance of genomic stability.
- Biological function summary
As a DNA damage response mediator 53BP1 functions to recognize and bind to sites of double-strand breaks in DNA promoting non-homologous end joining (NHEJ). This protein is a component of a larger protein complex that recruits factors necessary for DNA repair. Its interaction with DNA lesions also involves chromatin remodeling aiming to facilitate successful repair of the DNA. 53BP1 works in concert with other proteins such as H2AX and MDC1.
- Pathways
Researchers observe 53BP1 in processes such as the DNA damage checkpoint and repair pathways. This protein significantly contributes to the pathways involved in maintaining cell cycle checkpoints. It interacts with proteins like ATM and BRCA1 coordinating the cellular response to DNA damage and ensuring that genomic integrity checks occur properly before cell cycle progression continues. These interactions place 53BP1 at a pivotal position in decision-making between repair pathways.
- Associated diseases and disorders
Researchers often focus on 53BP1's involvement in cancer development and therapy resistance due to its role in DNA repair. Alterations or deficiencies in 53BP1 levels can contribute to increased susceptibility to tumor development affecting processes like breast cancer and ovarian cancer through its relationship with BRCA1. The improper function of 53BP1 in DNA repair pathways may lead to an accumulation of genetic mutations driving tumorigenesis and impacting the efficacy of certain cancer treatments.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab21083)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 53BP1 with ab21083 at 1/4000 dilution. Nuclear staining is observed. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western blot - Anti-53BP1 antibody (ab21083)
5% SDS-PAGE
All lanes: Western blot - Anti-53BP1 antibody (ab21083) at 1/500 dilution
Lane 1: 53BP1 shRNA non-transfected HeLa whole cell extracts at 50 µg
Lane 2: 53BP1 shRNA transfected HeLa whole cell extracts at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 214 kDa
Observed band size: 350 kDa
Western blot - Anti-53BP1 antibody (ab21083)
5% SDS-PAGE.
Running conditions: 80V, 15min; 140V, 40min.
Transfer condition: Semi-dry, 18 V, 60min (Nitrocellulose membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60min.
Primary antibody incubation: 1/2000, 4℃, overnight.
Secondary antibody incubation: Rabbit IgG antibody (HRP), 1/10,000, RT, 1hr.
Washing condition: 5 ml TBST, 4 x 5min.
Exposure system: Trident plus Western HRP Substrate.
All lanes: Western blot - Anti-53BP1 antibody (ab21083) at 1/2000 dilution
Lane 1: 293T whole cell lysate/extract at 30 µg
Lane 2: A431 whole cell lysate/extract at 30 µg
Lane 3: HeLa whole cell lysate/extract at 30 µg
Lane 4: HepG2 whole cell lysate/extract at 30 µg
Lane 5: A375 whole cell lysate/extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 214 kDa
Observed band size: 350 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab21083)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling 53BP1 with ab21083 at 1/4000 dilution. Nuclear staining is observed. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western blot - Anti-53BP1 antibody (ab21083)
5% SDS-PAGE
All lanes: Western blot - Anti-53BP1 antibody (ab21083) at 1/500 dilution
Lane 1: Neuro2A whole cell extracts at 30 µg
Lane 2: C8D30 whole cell extracts at 30 µg
Lane 3: NIH-3T3 whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 214 kDa
Observed band size: 350 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab21083)
ab21083 (2µg/ml) staining 53BP1 in human Brain: Cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is ubiquitous nuclear staining throughout.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Anti-53BP1 antibody (ab21083)
53BP1 Western blot staining using rabbit Anti-53BP1 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 15840649).
In Western blot, anti-53BP1 antibody (Anti-53BP1 antibody [RM1222] ab320755, left) and (ab21083, right) were used at 1/1000. A band was observed at 350 kDa in wild-type HAP1 cell lysates whereas no signal was observed at this size in the 53BP1 knockout cell line.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-53BP1 antibody [RM1222] (Anti-53BP1 antibody [RM1222] ab320755) at 1/1000 dilution
Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg
Lane 2: 53BP1 knockout HAP1 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 350 kDa, 124 kDa
Exposure time: 26s
Western blot - Anti-53BP1 antibody (ab21083)
53BP1 Western blot staining using rabbit Anti-53BP1 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 15840649).
In Western blot, anti-53BP1 antibody (Anti-53BP1 antibody [RM1222] ab320755, left) and (ab21083, right) were used at 1/1000.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-53BP1 antibody [RM1222] (Anti-53BP1 antibody [RM1222] ab320755) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 40 µg
Lane 2: Human testis tissue lysate at 40 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Mouse placenta tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 350 kDa, 124 kDa
Exposure time: 180s
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