Anti-53BP1 antibody (ab36823) is a rabbit polyclonal antibody that is used to detect 53BP1 in Western Blot, IP, IHC-P. Suitable for Human, Mouse samples.
- Over 140 publications
- Trusted since 2006
Images
Publications
Filter by
- Cell death discovery 9:4262023RMI1 facilitates repair of ionizing radiation-induced DNA damage and maintenance of genomic stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLianying Fang,Yuxiao Sun,Mingxin Dong,Mengmeng Yang,Jianxiu Hao,Jiale Li,Huanteng Zhang,Ningning He,Liqing Du,Chang XuPubMed 38007566
- British journal of cancer 129:1829-18402023DNA damage response inhibitors enhance tumour treating fields (TTFields) potency in glioma stem-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAurelie Vanderlinden,Callum G Jones,Katie N Myers,Ola Rominiyi,Spencer J CollisPubMed 37777579
- Frontiers in oncology 13:12025852023Lestaurtinib inhibits Citron kinase activity and medulloblastoma growth through induction of DNA damage, apoptosis and cytokinesis failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGianmarco Pallavicini,Giorgia Iegiani,Roberta Parolisi,Alessia Ferraro,Francesca Garello,Valeria Bitonto,Enzo Terreno,Marta Gai,Ferdinando Di CuntoPubMed 37404750
- Journal of cellular physiology 238:1836-18492023Carbon ions trigger DNA damage response to overcome radioresistance by regulating β-catenin signaling in quiescent HeLa cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua Zhang,Yi Xie,Xiaoyi Liu,Lu Gan,Pingping Li,Zhihui Dou,Cuixia Di,Hong Zhang,Jing SiPubMed 37334439
- International journal of biological sciences 19:3099-31142023Extracellular vesicles carrying miR-6836 derived from resistant tumor cells transfer cisplatin resistance of epithelial ovarian cancer via DLG2-YAP1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYazhu Zou,Zitong Zhao,Jingjing Wang,Liying Ma,Yi Liu,Li Sun,Yongmei SongPubMed 37416779
- Cell reports 42:1123722023NAD depletion mediates cytotoxicity in human neurons with autophagy deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCongxin Sun,Elena Seranova,Malkiel A Cohen,Miruna Chipara,Jennie Roberts,Dewi Astuti,Adina M Palhegyi,Animesh Acharjee,Lucia Sedlackova,Tetsushi Kataura,Elsje G Otten,Prashanta K Panda,Samuel Lara-Reyna,Miriam E Korsgen,Kevin J Kauffman,Alejandro Huerta-Uribe,Malgorzata Zatyka,Luiz F S E Silva,Jorge Torresi,Shupei Zhang,Georgina W Hughes,Carl Ward,Erich R Kuechler,David Cartwright,Sergey Trushin,Eugenia Trushina,Gaurav Sahay,Yosef Buganim,Gareth G Lavery,Joerg Gsponer,Daniel G Anderson,Eva-Maria Frickel,Tatiana R Rosenstock,Timothy Barrett,Oliver D K Maddocks,Daniel A Tennant,Haoyi Wang,Rudolf Jaenisch,Viktor I Korolchuk,Sovan SarkarPubMed 37086404
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22043882023Human Endonuclease ANKLE1 Localizes at the Midbody and Processes Chromatin Bridges to Prevent DNA Damage and cGAS-STING Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuadong Jiang,Nannan Kong,Zeyuan Liu,Stephen C West,Ying Wai ChanPubMed 36825683
- Cell reports 42:1120622023GEN1 promotes common fragile site expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnaid Benitez,Marie Sebald,Radhakrishnan Kanagaraj,Monica C Rodrigo-Brenni,Ying Wai Chan,Chih-Chao Liang,Stephen C WestPubMed 36729836
- Cells 12:2023Correlative Multi-Modal Microscopy: A Novel Pipeline for Optimizing Fluorescence Microscopy Resolutions in Biological Applications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimone Pelicci,Laura Furia,Pier Giuseppe Pelicci,Mario FarettaPubMed 36766696
- Journal of radiation research 64:471-4792023Effect of curcumin on γ-ray-induced cell response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNora Kostova,Albena Staynova,Ljubomira Popova-Hadjiiska,Dimka Georgieva,Ilonka Ivanova,Nevena Aneva,Margarita Atanasova,Rositsa HristovaPubMed 36634350
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TP53BP1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q12888
Consider this alternative
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/25000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:17190600, PubMed:21144835, PubMed:22553214, PubMed:23333306, PubMed:27153538, PubMed:28241136, PubMed:31135337, PubMed:37696958). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23333306, PubMed:23727112, PubMed:27153538, PubMed:31135337). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:17190600, PubMed:23760478, PubMed:27153538, PubMed:28241136). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).
Alternative names
TP53-binding protein 1, 53BP1, p53-binding protein 1, p53BP1, TP53BP1
Recommended products
Anti-53BP1 antibody (ab36823) is a rabbit polyclonal antibody that is used to detect 53BP1 in Western Blot, IP, IHC-P. Suitable for Human, Mouse samples.
- Over 140 publications
- Trusted since 2006
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TP53BP1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q12888
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-53BP1 antibody (ab36823) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse samples.
What is the molecular weight of 53BP1?
Anti-53BP1 (ab36823) specifically detects a band for 53BP1 (UniProt: Q12888) at a molecular weight of 214kDa.
Trusted by the scientific community
Anti-53BP1 (ab36823) was first used in a scientific publication in 2006 and has been cited over 140 times in peer-reviewed journals.
Reviewed by scientists
Anti-53BP1 (ab36823) has over 10 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
53BP1 also known as p53-binding protein 1 is a protein that plays an important role in DNA damage response. The 53BP1 protein has a molecular weight of approximately 213 kDa. It is a nuclear protein and it mainly expresses in cells during the DNA damage repair processes. Researchers commonly utilize immunofluorescence and anti-53BP1 antibodies to detect its presence especially for studying DNA repair mechanisms. 53BP1 proves important for maintenance of genomic stability.
- Biological function summary
As a DNA damage response mediator 53BP1 functions to recognize and bind to sites of double-strand breaks in DNA promoting non-homologous end joining (NHEJ). This protein is a component of a larger protein complex that recruits factors necessary for DNA repair. Its interaction with DNA lesions also involves chromatin remodeling aiming to facilitate successful repair of the DNA. 53BP1 works in concert with other proteins such as H2AX and MDC1.
- Pathways
Researchers observe 53BP1 in processes such as the DNA damage checkpoint and repair pathways. This protein significantly contributes to the pathways involved in maintaining cell cycle checkpoints. It interacts with proteins like ATM and BRCA1 coordinating the cellular response to DNA damage and ensuring that genomic integrity checks occur properly before cell cycle progression continues. These interactions place 53BP1 at a pivotal position in decision-making between repair pathways.
- Associated diseases and disorders
Researchers often focus on 53BP1's involvement in cancer development and therapy resistance due to its role in DNA repair. Alterations or deficiencies in 53BP1 levels can contribute to increased susceptibility to tumor development affecting processes like breast cancer and ovarian cancer through its relationship with BRCA1. The improper function of 53BP1 in DNA repair pathways may lead to an accumulation of genetic mutations driving tumorigenesis and impacting the efficacy of certain cancer treatments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunoprecipitation - Anti-53BP1 antibody (ab36823)
53BP1 was immunoprecipitated from 1 mg HEK293T whole cell lysate with ab36823 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using Anti-53BP1 antibody [BL-250-1H11] ab243868 at a 1/1000 dilution.
Lane 1: ab36823 IP in HEK293T whole cell lysate.
Lane 2: Contol IgG in HEK293T whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-53BP1 antibody (ab36823)
Predicted band size: 214 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab36823)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate cancer tissue, labeling CTCF with ab36823 at a 1/5000 dilution. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab36823)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cancer tissue, labeling CTCF with ab36823 at a 1/1000 dilution. Detection: DAB.
Western blot - Anti-53BP1 antibody (ab36823)
Detection: Chemiluminescence.All lanes: Western blot - Anti-53BP1 antibody (ab36823) at 0.04 µg/mL
Lane 1: HeLa cell nuclear extract at 50 µg
Lane 2: HeLa cell nuclear extract at 5 µg
Lane 3: TCMK1 mouse cell nuclear extract at 15 µg
Predicted band size: 214 kDa
Exposure time: 30s
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-53BP1 antibody (ab36823)
53BP1 western blot using anti-53BP1 antibody ab36823. Publication image and figure legend from Onyango, D. O., Lee, G., et al., 2017, Oncotarget, PubMed 29212152.
ab36823 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab36823 please see the product overview.
Effects of PRPF8 and PlaB on BRCA1 levels and SSA in 53BP1-deficient cellsA. PlaB treatment causes a marked reduction in both BRCA1 protein and RNA. Cells were transfected with siRNA and cultured 3 days, and PlaB and DRB treatments were for 16 hr, prior to protein or RNA extraction, which were probed for BRCA1 and Actin by immunoblot and qRT-PCR, respectively. B. Loss of 53BP1 partially suppresses the SSA defect caused by PRPF8 depletion, but not PlaB treatment. Using Cas9, a derivative of the SA-GFP cell line with loss of 53BP1 (53BP1KO line) was generated, and reporter assays were performed as in Figure 1B and 1D. Shown are GFP+ frequencies normalized to parallel siCTRL or siCTRL/DMSO treated wells. Also shown is immunoblot confirmation of depletion of BRCA1 and PRPF8 by the respective siRNAs, and loss of 53BP1 in the 53BP1KO line. siPRPF8-2 graph, *P < 0.0001, parental line N = 15, 53BP1KON = 12. siPRPF8-4 graph *P = 0.001, N = 6. PlaB graph, parental line N = 12, 53BP1KON = 9.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com